Feasibility of sex-sorting sperm from the white and the black rhinoceros (Ceratotherium simum, Diceros bicornis)

Behr, B.; Rath, Detlef; Mueller, Peter P.; Hildebrandt, Thomas B.; Goeritz, Frank; Braun, Beate C.; Leahy, Tamara; Graaf, S.P. de; Maxwell, W.M.C.; Hermes, Robert

doi:10.1016/j.theriogenology.2009.03.001

Cited by 15 publications

(16 citation statements)

References 47 publications

(53 reference statements)

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“…The technology has by now become commercial and is widely used in the cattle industry. Sperm sex sorting has also been attempted in white and black ( Diceros bicornis ) rhinoceroses [Behr et al, ] but to date no pregnancy has been reported. Further pursuing this technology and using the sorted spermatozoa for ICSI would increase the chances of generating female embryos that can be transferred.…”

Section: The Hope In Artificial Gametesmentioning

confidence: 99%

Rewinding the process of mammalian extinction

et al. 2016

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ZOO Dvůr Kr alov e, Dvůr Kr alov e nad Labem, Czech RepublicWith only three living individuals left on this planet, the northern white rhinoceros (Ceratotherium simum cottoni) could be considered doomed for extinction. It might still be possible, however, to rescue the (sub)species by combining novel stem cell and assisted reproductive technologies. To discuss the various practical options available to us, we convened a multidisciplinary meeting under the name "Conservation by Cellular Technologies." The outcome of this meeting and the proposed road map that, if successfully implemented, would ultimately lead to a self-sustaining population of an extremely endangered species are outlined here. The ideas discussed here, while centered on the northern white rhinoceros, are equally applicable, after proper adjustments, to other mammals on the brink of extinction. Through implementation of these ideas we hope to establish the Conflicts of interest: None.Oliver A. Ryder and Thomas B. Hildebrandt are of equal seniority.Co-authors, other than first and the two senior authors, are listed by alphabetical order.

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Section: The Hope In Artificial Gametesmentioning

confidence: 99%

Rewinding the process of mammalian extinction

et al. 2016

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“…Anel et al, 2008) or they may come with or without the relevant secretions from all accessory glands. In elephants this is manifested by occasional ejaculates with very sticky consistency indicating that high level of secretions from the bulbourethral gland are present (personal observation) whereas in rhinoceros it is manifested by high viscosity of the ejaculate (Behr et al, 2009b). In rhinoceros, measuring alkaline phosphatase in the ejaculate was suggested as a mean to identify true ejaculates (Roth et al, 2010).…”

Section: The Malementioning

confidence: 99%

“…In camelids, possibly due to the absence of vesicular glands, sperm is also fairly viscous but it can be enzymatically liquefied (Bravo et al, 2000). Similar enzymatic liquefaction was also helpful when attempting to separate rhinoceros sperm from the seminal plasma, something that cannot be done efficiently with centrifugation alone in some of the ejaculates (Behr et al, 2009b). The volume and concentration also vary by several orders of magnitude among species.…”

Section: The Malementioning

confidence: 99%

“…This technique has been tested in various wildlife species such as elephants, rhinoceros, dolphins and non-human primates O'Brien & Robeck, 2006;Behr et al, 2009a;Behr et al, 2009b;Hermes et al, 2009a). Sperm sorting machines, however, are very expensive, scarce and usually situated far away from where the sperm donor and recipient are located.…”

Section: Semen Freezingmentioning

confidence: 99%

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Genome Banking for Vertebrates Wildlife Conservation

Saragusty¹

2012

Current Frontiers in Cryobiology

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“…Over the last decade tremendous strides have been made in establishing successful assisted reproductive techniques for both African and Asian rhinoceros species (Hermes et al, ; Hildebrandt et al, ; Stoops et al, ). Technologies regularly utilized in the livestock industry to influence the sex ratio of offspring prior to implantation, such as cyotometric sexing of spermatozoa and embryo sexing, may soon play a part in the genetic and demographic management of captive rhinoceros populations (Behr et al, , ; O'Brien et al, , ). Therefore, establishment of a validated molecular sexing method using very small quantities of rhinoceros template DNA will be of utility as in vitro fertilization, embryo maturation, and embryo transfer become established in the different rhinoceros species.…”

Section: Introductionmentioning

confidence: 99%

Early fetal sexing in the rhinoceros by detection of male‐specific genes in maternal serum

Stoops

Winget

DeChant

et al. 2018

Molecular Reproduction Devel

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Genetic sexing of animals with long gestation time benefits the management of captive populations. Here, X and Y chromosome-specific primers, based on equine gene sequencing data, were developed and tested on captive rhinoceroses (10 males, 20 females) representing four species (Diceros bicornis, Certaotherium simum simum, Rhinoceros unicornis, and Dicerorhinus sumatrensis). The Y chromosome-specific primer set targeted SRY (Sex-determining region Y), and amplified a 177-bp product following PCR of DNA extracted from males, but not females, of all species. A primer set based on the equine AMEL (Amelogenin) gene resulted in a 232-bp product following PCR of all rhinoceros species. These gene-specific primer sets were then evaluated for their ability to determine gender in cell-free DNA from rhinoceros serum. Modifications to the original extraction and PCR protocols were required to obtain sufficient DNA quantities from serum, and both DNA yield and PCR amplification were substantially reduced or absent following multiple freeze-thaw cycles of serum. When fresh serum from 14 pregnant rhinoceroses (ultimately bearing seven male and seven female calves), representing four species at different stages of gestation (Days 61-490), were probed in a PCR-based assay, an accuracy of 71% was achieved for male-specific gene detection of SRY, which improved to 100% by including a reamplification step into the protocol. Such early sex determination should be a valuable tool for current management practices as well as future assisted reproduction of rhinoceroses.

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Feasibility of sex-sorting sperm from the white and the black rhinoceros (Ceratotherium simum, Diceros bicornis)

Cited by 15 publications

References 47 publications

Rewinding the process of mammalian extinction

Rewinding the process of mammalian extinction

Genome Banking for Vertebrates Wildlife Conservation

Early fetal sexing in the rhinoceros by detection of male‐specific genes in maternal serum

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