Feasibility of Shortening Respiratory Isolation with a Single Sputum Nucleic Acid Amplification Test

Abstract

A single first-sputum NAA testing can rapidly and accurately identify the subset of patients with suspected TB who require RI according to serial sputum smears. Its potential use to shorten RI time does not preclude the need to obtain subsequent specimens for culture.

Rapid Detection of Mycobacterium tuberculosis and Rifampin Resistance by Use of On-Demand, Near-Patient Technology

“…Each module within the GeneXpert instrument operates independently, which enables the user to test each sputum sample as it arrives in the laboratory instead of saving samples for batch processing. This important feature can potentially result in dramatically reduced turnaround times for tuberculosis detection, allowing decisions about respiratory isolation and treatment to be made in real time (3). Falsepositive results, often caused by carryover of amplified target, are mitigated by the use of closed cartridges that do not require any manual pipetting after the sample has been added to the cartridge.…”

Shortening Isolation of Patients With Suspected Tuberculosis by Using Polymerase Chain Reaction Analysis: A Nationwide Cross-sectional Study

“…PCRnegative/smear-positive patients in the analysis on the same sample did not occur in our analysis. Prior evaluations found that PCR identified all patients with tuberculosis with AFB in serial smears [22,26], although 1 study evaluating a 1-, 2-, and 3-sample PCR (GeneXpert MTBC/RIF) approach missed 1 of 6 smear-positive tuberculosis cases with the 1-sample approach [21]. Although a PCR-based algorithm for isolation Up to 3 initial results are included (within 14 days before or after the first culture-positive sample).…”

“…Although Centers for Disease Control and Prevention guidelines state that the 3 sputum samples necessary for reliable smear microscopy can be collected over 2 days [5], the actual process often takes up to 5 days [26]. A conventional PCR result will most often be available within 2 days; thus, the benefit of allowing discontinuation of isolation on the basis of single-sputum PCR results is a reduction of the time spent in isolation for patients who do not have contagious tuberculosis.…”

Comparison of the Diagnostic Performance of Two Real-Time PCR Systems for the Detection of Mycobacterium tuberculosis in Clinical Samples

“…Yalancı pozitif sonuçlar teknisyen hataları, üretim kusurları, sıvılar ile ilgili sorunlar ve örnekte inhibitör varlığından kaynaklanabileceği ileri sürülmektedir (24) . Taylor ve ark.…”