2023
DOI: 10.1016/j.ijhydene.2022.12.231
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Feasibility of successive hydrogen and methane production in a single-reactor configuration of batch anaerobic digestion through bioaugmentation and stimulation of hydrogenase activity and direct interspecies electron transfer

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Cited by 17 publications
(2 citation statements)
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“…TW80 has also been proven to have the potential to boost hydrogenase and hydrolytic enzyme activities when applied in combination with conductive materials that enhance electron transfer during fermentation [ 70 ]. Moreover, the addition of iron to the methanogenesis system has been observed to increase and maintain stable methane-producing activity [ 71 , 72 ], as iron is an essential part of several key enzymes required for methanogens [ 73 ]. Despite the identified positive synergism, however, there are still gaps in the understanding of the synergistic mechanism between iron and surfactants on AD performance, especially regarding activity related to methanogenesis, which is the key process for biogas generation.…”
Section: Resultsmentioning
confidence: 99%
“…TW80 has also been proven to have the potential to boost hydrogenase and hydrolytic enzyme activities when applied in combination with conductive materials that enhance electron transfer during fermentation [ 70 ]. Moreover, the addition of iron to the methanogenesis system has been observed to increase and maintain stable methane-producing activity [ 71 , 72 ], as iron is an essential part of several key enzymes required for methanogens [ 73 ]. Despite the identified positive synergism, however, there are still gaps in the understanding of the synergistic mechanism between iron and surfactants on AD performance, especially regarding activity related to methanogenesis, which is the key process for biogas generation.…”
Section: Resultsmentioning
confidence: 99%
“…As acidogenesis can be evaluated using methyl red test, acetogenesis can also be checked and verified using methyl red and gas production tests [12,58]. Hydrogenase enzymes are crucial in the usage and generation of hydrogen gas and gas production test give a hint if hydrogen is being produced or not [59] [60]. Dehydrogenase enzyme Facilitates the transformation of formate into carbon dioxide and hydrogen, providing acetogens with an alternative method to generate substances for methanogenesis, this step can also be verified using gas production test [61].…”
Section: Biochemical Tests For Isolated Strainsmentioning
confidence: 99%