2009
DOI: 10.1128/jcm.00081-09
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Feasibility of the GenoType MTBDR sl Assay for Fluoroquinolone, Amikacin-Capreomycin, and Ethambutol Resistance Testing of Mycobacterium tuberculosis Strains and Clinical Specimens

Abstract: The new GenoTypeThe worldwide emergence of extensively drug-resistant tuberculosis (XDR TB, resistant at least to rifampin and isoniazid, a fluoroquinolone [FLQ], and one of the three injectable second-line drugs amikacin [AM], kanamycin [KM], and capreomycin [CM]) is a serious global health problem (20,25). In the World Health Organization fourth global report on drug resistance (25), it was documented that more than 45 countries have reported XDR cases. The actual incidence could be underestimated, because s… Show more

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Cited by 232 publications
(256 citation statements)
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“…This is similar to previously reported data (5,17), suggesting that this assay is feasible for use in areas with high rates of drug resistance. There was 95.5% concordance between results of conventional sequencing and the MTBDRsl assay, excluding unreadable results and mixed allelic variants, demonstrating a high specificity for the assay.…”
Section: Discussionsupporting
confidence: 80%
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“…This is similar to previously reported data (5,17), suggesting that this assay is feasible for use in areas with high rates of drug resistance. There was 95.5% concordance between results of conventional sequencing and the MTBDRsl assay, excluding unreadable results and mixed allelic variants, demonstrating a high specificity for the assay.…”
Section: Discussionsupporting
confidence: 80%
“…Bacteriological methods are highly sensitive and specific but very labor-intensive and time-consuming. Consequently, molecular diagnostic methods are being introduced into routine use for the rapid detection of FQ resistance by identification of mutations in specific gyrA and gyrB genes regions (QRDR) (17,46). This study is the first to assess the effectiveness of both a noncommercial DNA sequencing and a commercially available molecular diagnostics system (GenoType MTBDRsl) for the rapid detection of M. tuberculosis resistance to FQ, conducted in the Russian Federation (a middle-incidence region with high rates resistance to anti-TB drugs).…”
Section: Discussionmentioning
confidence: 99%
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“…Previous evaluations of the GenoType kits have documented their performance in detecting resistance to first-and second-line antituberculosis (anti-TB) drugs in different continents, like Africa, Asia, and Europe (2,3,4,5). From these studies, it became clear that there are geographic variations in the mutations that are associated with drug resistance in Mycobacterium tuberculosis (6).…”
Section: N June 2008 the World Health Organization (Who) Endorsedmentioning
confidence: 99%
“…In addition to fluoroquinolone mono-resistant M. tuberculosis isolates, such tests could be used to rapidly identify extensively drug-resistant tuberculosis (XDR-TB; defined as resistance to at least isoniazid and rifampicin plus a fluoroquinolone and a second-line injectable agent such as capreomycin, amikacin and kanamycin). 28 -30 A line probe assay has been developed to detect fluoroquinolone-resistance in M. tuberculosis, but its sensitivity ranges from 75.6% to 89.5% when compared with conventional phenotypic susceptibility methods, 19,31,32 and 87% to 100% when compared with DNA sequencing. 31,33 The purpose of this review was to characterize all DNA gyrase gene mutations described in M. tuberculosis clinical strains and to distinguish those associated with fluoroquinolone resistance and those reported in fluoroquinolone-susceptible strains.…”
Section: Introductionmentioning
confidence: 99%