One of the most promising techniques for condition monitoring of high voltage equipment insulation is partial discharge (PD) measurement using radio frequency (RF) antenna. Nevertheless, the accuracy of monitoring, classification, localization, or lifetime estimation could be negatively affected due to the interferences and noises measured simultaneously and contaminate the RF signals. Therefore, to achieve high accuracy of PD assessment, exploiting the denoising algorithms is inevitable. Hence, this paper seeks to introduce a new technique to suppress white noise, the most prevalent type of noise, especially for RF signals. In the proposed method, the ability of artificial neural network (ANN) in curve fitting is applied to denoising of different types of measured RF signals emitted from PD sources including ‘crack’, ‘internal void’, in the insulator discs and ‘sharp points’ from external hardware. The processes of denoising for named signals with the proposed method are carried out, and the obtained results are compared with the outputs of a wavelet transform-based method named energy conversation-based thresholding. In all tested signals, the proposed technique showed superior denoising capability.