A biometric system offers automatic identification of an individual basedon characteristic possessed by the individual. Biometric identification systems are often categorized as physiological or behavioural characteristics.Gait as one of the behavioural biometric recognition aims to recognizean individual by the way he/she walk. In this paper we propose genderclassification based on human gait features using wavelet transform andinvestigates the problem of non-neutral gait sequences; Coat Wearing andcarrying bag condition as addition to the neutral gait sequences. We shallinvestigate a new set of feature that generated based on the Gait Energy Image and Gait Entropy Image called Gait Entropy Energy Image(GEnEI). Three different feature sets constructed from GEnEI basedon wavelet transform called, Approximation coefficient Gait EntropyEnergy Image, Vertical coefficient Gait Entropy Energy Image and Approximation & Vertical coefficients Gait Entropy Energy Image Finallytwo different classification methods are used to test the performance ofthe proposed method separately, called k-nearest-neighbour and SupportVector Machine. Our tests are based on a large number of experimentsusing a well-known gait database called CASIA B gait database, includes124 subjects (93 males and 31 females). The experimental result indicatesthat the proposed method provides significant results and outperform thestate of the art.