Features of Anti-radical Activity and Antioxidant Sperm Status in Men Living in the Western Urals

Abstract

Inflammatory diseases in the urogenital tract of men living in different climatic conditions often cause infertility. The study aims to assess the antioxidant status and antiradical activity of sperm in men with chronic prostatitis living in the Western Urals. 55 men aged 23 to 38 years with inflammatory diseases of the prostate gland living in the Western Urals were examined. Group 1 consisted of 25 men with chronic prostatitis in remission, group 2 was composed of 30 people with chronic prostatitis at the acute stage. The level of total antiradical activity of sperm (TAAS) was studied using the SF PE-5300 (Russia), the activity of superoxide dismutase (SOD) was studied on the enzyme immunoassay analyzer Sunrise (Tecan, Austria). Results are presented as arithmetic mean (M), standard error (m) and 95 % confidence interval for mean (95 % CI). To test the null hypotheses about the equality of mean values between two independent groups with a normal distribution, the two-sample Student's test was used. The significance level at which the null hypotheses were tested was 0.05. According to the assessment of the antioxidant status of sperm, there was a statistically significant (p = 0.039) 20 % increase in the total antiradical activity of sperm in patients with an acute inflammatory process in the prostate gland compared to the similar indicator identified in men without symptoms of the acute inflammation. In the group of patients with acute chronic prostatitis, the share of samples with a significant increase in the level of total antiradical activity of seminal plasma compared to the reference level was 33.3 % versus 23.3 % in men with chronic prostatitis during the remission; the frequency of excess was 1.5 times. The evaluation of sperm parameters showed that the activity of superoxide dismutase in both groups was within the reference range. In the seminal plasma of men with chronic prostatitis during an exacerbation, in 8.8 % of samples, the activity of superoxide dismutase was lower, and in patients with the chronic process in the prostate gland during the remission, the activity of superoxide dismutase was below the reference level in 3.3 % of the samples, the frequency of a decrease was 2.7. Thus, with chronic prostatitis, the imbalance in the oxidation-antioxidation system plays a key role in the development of impaired spermatogenesis, and markers of redox processes can serve as informative indicators of the