Features of Changes in Fatty Acids Composition of Tissues in Different Models of Experimental Type 1 Diabetes

Osadchuk, Yu.S.; Chaikovsky, Yu. B.; Natrus, L.V.; Bryuzgina, T.S.

doi:10.32345/2664-4738.3-4.2018.02

Cited by 2 publications

(1 citation statement)

References 8 publications

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“…Декомпозиція балок гепатоцитів, наявність сполучнотканинних містків (порто-центральних і порто-портальних) за відсутності ознак регенерації у вигляді формування несправжніх часточок свідчить про розвиток фіброзу [5]. Імовірним механізмом розвитку жирової дистрофії гепатоцитів є декомпозиція клітинних мембран і загибель гепатоцитів, що в сукупності з розвитком сполучної тканини відповідає наслідкам хронічної ішемізації органа в разі обмеження портального кровотоку [5,8].…”

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Partial hepatic portal vein ligation: morphological assessment of prehepatic portal hypertension modelling

Godik¹,

Levytskyi²,

Diehtiarova³

et al. 2023

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Portal hypertension is an increase in pressure in the hepatic portal vein system. The most common animal model of prehepatic portal hypertension in use today is partial ligation of the hepatic portal vein in rats but existing studies focus on determining the short-term effects of partial ligation of the hepatic portal vein. Purpose - to evaluate the model of prehepatic portal hypertension by means of a histological study of partial portal vein ligation influence on liver tissue. Materials and methods. Male Wistar rats (n=45), aged 6 weeks and weighing 150±15 grams, were included in the study. The animals were divided into 3 groups: the Group 1 - partial ligation of the portal vein of the liver was performed (formation of stenosis; n=15), the Group 2 - ligation of the portal vein of the liver without its obstruction was performed (pseudo-operated; n=15), the Group 3 - a control group (intact animals, n=15). The rats were withdrawn from the experiment six months after the operation. For histological examination, liver fragments were taken, after standard preparation of the preparations, photographed with an OLYMPUS BX51 light microscope and examined with a PEM-125k electron microscope. The obtained microphotographs were processed and analyzed using the biomedical image processing software ImageJ v.1.50 (National Institutes of Health, USA). Digital data were analyzed in Graphpad Prizm v. 8.3 (Graphpad, USA) statistical package. Results. In rats of the Group 1 the presence of large droplet and total fatty dystrophy of hepatocytes in the center of the lobules was established. The development of connective tissue with the formation of centro-central and porto-central septa was observed. The prolonged effect of partial ligation of the portal vein most closely corresponded to the picture of zonal fatty parenchymal dystrophy of the liver and balloon degeneration of hepatocytes with subsequent fibrosis development. In animals of the Group 1, the distribution of the specific number of hepatocyte nuclei differed from the normal one (p=0.0124), the presence of differences in this indicator between the animals of the three groups was established (p<0.005). Histological examination of the liver of the Group 2 rats revealed preservation of the histological structure of the organ, with moderate changes. Rats of the Group 3 showed normal histoarchitectonics of the organ. Conclusions. The homogeneity of changes in the liver and their reproducibility indicate the stability of the developed model and its suitability for further development of treatment methods. The experiments with laboratory animals were provided in accordance with all bioethical norms and guidelines. No conflict of interests was declared by the authors.

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Section: обговоренняunclassified

Partial hepatic portal vein ligation: morphological assessment of prehepatic portal hypertension modelling

Godik¹,

Levytskyi²,

Diehtiarova³

et al. 2023

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The Pathogenetic Rationale the Ways of Experimental Type 2 Diabetes Mellitus Modeling

et al. 2019

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Relevance. When modeling experimental type 2 diabetes mellitus (T2DM), various schools and research groups receive significant variability in tissue changes, which is difficult to compare and extrapolate as a specific pathogenic or pharmacological effect. The lack of standard operating procedures agreed upon in the scientific community greatly complicates the interpretation of the result. Therefore, the creation of a uniquely pathogenetic animal model of CD2 in animals is an urgent task. Objective. Determination of the complex of exogenous effects for the most efficient reproduction of the experimental model of T2DM in rats and the pathogenetic justification of the changes in the body to study the central mechanisms of homeostasis regulation and their pharmacological correction. Materials and methods. T2DM was model in rats by feeding a high-fat diet (HFD) with additional streptozotocin induction. The model was evaluated by anthropometric measurements, studies of biochemical parameters, an insulin tolerance test, analysis of the spectrum of fatty acids in the composition of tissues. Statistical data processing was performed with using the IBM SPSS Statistics 23. Results. The obtained data were compared with our studies of the lipid metabolism of patients with varying degrees of diabetic retinopathy on the background of T2DM and analysis of the information content of biochemical markers to assess lipid metabolic disturbances on the background of hyperglycemia in patients. It was found that the simulation in rats of experimental T2DM by a food load of HFD (45%) + fructose 20% for 3 months followed by a single induction of streptozotocin (25 mg/kg) causes changes in lipid and carbohydrate metabolism in animals similar changes which are in the human with a diagnosed long-term diabetes and the development of microvascular complications. Conclusion. We determined the optimal combination of effects and developed a consistent experimental load scheme that allows us to obtain a symptom model of type 2 diabetes in an animal experiment. Using the diagnostic algorithm, which includes physiological and laboratory methods, the degree of damage to organs and systems was determined, a comparison was made with the level of metabolic disorders in patients with T2DM and people without diabetes. The given model is a pathogenetically grounded approach for further study of the central mechanisms of homeostasis regulation and their pharmacological correction

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Features of Changes in Fatty Acids Composition of Tissues in Different Models of Experimental Type 1 Diabetes

Cited by 2 publications

References 8 publications

Partial hepatic portal vein ligation: morphological assessment of prehepatic portal hypertension modelling

Partial hepatic portal vein ligation: morphological assessment of prehepatic portal hypertension modelling

The Pathogenetic Rationale the Ways of Experimental Type 2 Diabetes Mellitus Modeling

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