Features of lettuce transgenic plants with the ifn-α2b gene regenerated after Agrobacterium rhizogenes-mediated transformation

Матвєєва, Н. А.; Shakhovskij, A. M.; Кучук, М. В.

doi:10.3103/s0095452712030073

Cited by 3 publications

(3 citation statements)

References 32 publications

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“…In 10-14 days after transformation with the wild strain of A. rhizogenes root formation at a frequency of 59.4% was observed. In the case of using A. rhizogenes carrying pCB161 vector root formation incidence was 37.5% (Matvieieva, 2012b).…”

Section: Artemisia Dracunculus L (Tarragon)mentioning

confidence: 98%

“…We transferred human interferon-α2b gene (ifn-α2b) in Cichorium intybus, Artemisia tilesii, Bidens pilosa, Althaea officinalis, and Lactuca sativa (Matvieieva et al, 2009;Matvieieva et al, 2012a). The antiviral activity of extracts from transgenic plants and roots was studied (Matvieieva et al, 2012b).…”

Section: Antiviral Activity Of the Extracts Of Hairy Rootsmentioning

confidence: 99%

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Bacteria – Plant Interaction: Transgenic Roots for Human Health

Matvieieva,

Bohdanovych,

Duplij

2023

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plasmid -Tumor induced plasmid vir genesvirulence genes rol genesroot loci or root locus genes T-DNAregion (area) of transport DNA (T-DNA), which is transferred to plant cells by the action of virulence genes (vir) from the same plasmid IAAindoleacetic acid orfopen reading frame mismikimopine synthase sequences iaaHindole-3-acetamide hydrolase Acsagrocinopine synthase CFUcoliny forming unit DMBQ -2,6-dimethoxy-1,4-benzoquinone UV light -Ultraviolet light LBleft border RBright border CRISPR -Clustered Regularly Interspaced Short Palindromic Repeats Cas9 -CRISPR-associated nuclease MSCmultiple cloning sites Tra/trbtranscriptional activators B markerbacterial selectable marker Propromoter Repreporter gene FDSfarnesyl diphosphate synthase gene GFPgreen fluorescent protein ifn-α2bhuman interferon-α2b SMssecondary metabolites SH -Schenk & Hildebrandt medium MS -Murashige & Skoog medium B5 -Gamborg medium SODsuperoxide dismutase GPXglutathione peroxidase PALphenylalanine ammonia-lyase gene CHIchalcone isomerase gene FLSflavonol synthase gene DPPH -2,2-diphenyl-1-picrylhydrazyl ABTS -2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) ZFNszinc finger nucleases NLSnuclear localization signal p35S -Cauliflower Mosaic Virus promoter GUS FW β-glucuronidase, a product of the uidA gene, which converts a soluble colorless substrate 5-bromo-4-chloro-3-indolyl-β-D-glucuronic acid into an insoluble colored product chloro-bromoindigo FWfresh weight RSAradical scavenging activity CTABcetyltrimethylammonium bromide AgNPssilver nanoparticles

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Section: Artemisia Dracunculus L (Tarragon)mentioning

confidence: 98%

Section: Antiviral Activity Of the Extracts Of Hairy Rootsmentioning

confidence: 99%

Bacteria – Plant Interaction: Transgenic Roots for Human Health

Matvieieva,

Bohdanovych,

Duplij

2023

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“…These genes encode the secretory proteins Ag85b and ESAT6 of tuberculosis pathogen and can serve as inducers of immune response in humans and animals against the disease. Plants of carrots, lettuce, and rape that expressed the gene of human alpha-interferon were also created [31][32][33]. The protein alpha-interferon is involved in a complex of human nonspecific immune responses, especially against viral diseases.…”

mentioning

confidence: 99%

Cell genetic engineering: Transmission genetics of plants

Кучук

2017

Cytol. Genet.

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Abstract⎯Achievements of cell and genetic engineering that led to formation of a new genetics chaptertransmission genetics-have been described. Results have been analyzed and new opportunities in the field of transgenomic somatic hybrids and cybrid obtaining, production of transgenic plants with agronomic pharmaceutical application, development of transplastomic plants, and accumulation of recombinant proteins by using the transient expression of foreign genes in plants have been shown.

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USING OF Agrobacterium-MEDIATED TRANSFORMATION FOR THE BIOTECHNOLOGICAL IMPROVEMENT OF COMPOSITAE PLANTS.

Матвєєва¹

2015

Biotechnol. acta

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The review focused on the data concerning current state in the field of Compositae "hairy" roots and transgenic plants construction using A.tumefaciens-and A. rhizogenes-mediated transformation to obtain biologically active compounds, including recombinant proteins. The article presents data on the results of genetic transformation of Cichorium intybus, Lactuca sativa, Artemisia annua, Artemisia vulgaris, Calendula officinalis, Withania somnifera and other Compositae plants as well as studies on the artemisinin, flavonoids, polyphenols, fructans and other compounds accumulation in transgenic plants and roots. The data show that the use of biotechnological approaches for construction of "hairy" roots and transgenic plants with new features are of great interest. The possibility of increase in the accumulation of naturally synthesized bioactive compounds and recombinant proteins production via A. tumefaciens and A. rhizogenes-mediated transformation have been shown. In vitro cultivation of transgenic plants characterized by high level of bioactive compounds accumulation and synthesis of recombinant proteins makes it possible to obtain guaranteed pure raw material. Using of biotechnological approaches preserved natural populations of plants is particularly important for rare and endangered plant species.

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Features of lettuce transgenic plants with the ifn-α2b gene regenerated after Agrobacterium rhizogenes-mediated transformation

Cited by 3 publications

References 32 publications

Bacteria – Plant Interaction: Transgenic Roots for Human Health

Bacteria – Plant Interaction: Transgenic Roots for Human Health

Cell genetic engineering: Transmission genetics of plants

USING OF Agrobacterium-MEDIATED TRANSFORMATION FOR THE BIOTECHNOLOGICAL IMPROVEMENT OF COMPOSITAE PLANTS.

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