The rapid and accurate estimation of aboveground forest phytomass remains a challenging research task. In general, methods for estimating phytomass fall mainly into the category of field measurements performed by ground-based methods, but approaches based on remote sensing and ecological modelling have been increasingly applied. The aim is to develop the scientific and methodological framework for the remote sensing estimation of qualitative and quantitative characteristics of forest stands, using the combination of surveys and machine learning models to determine phytomass of forest stands and calculate the carbon balance. Even-aged stands of different tree species growing in the forest steppe zone of the East European Plain were chosen as test objects. We have applied the modernized methodological approaches to compare and integrate forest and tree stand characteristics obtained by ground-based and UAV-based comprehensive surveys; additionally, we developed computer vision models and methods for determining the same characteristics by remote sensing methods. The key advantage of the proposed methodology for remote monitoring and carbon balance control over existing analogues is the minimization of the amount of groundwork and, consequently, the reduction inlabor costs without loss of information quality. Reliable data on phytomass volumes will allow for operational control of the forest carbon storage, which is essential for decision-making processes. This is important for the environmental monitoring of forests and green spaces of various economic categories. The proposed methodology is necessary for the monitoring and control of ecological–climatic and anthropogenic–technogenic transformations in various landscapes. The development is useful for organizing the management of ecosystems, environmental protection, and managing the recreational and economic resources of landscapes with natural forests and forest plantations.