Fecal bacteria in coastal lakes: An anthropogenic contamination or natural element of microbial diversity?

Lew, Sylwia; Glińska-Lewczuk, Katarzyna; Burandt, Paweł; Grzybowski, M.; Obolewski, Krystian

doi:10.1016/j.ecolind.2023.110370

Cited by 7 publications

(3 citation statements)

References 56 publications

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“…However, water monitoring procedures have not been standardized, and reliable data for determining the abundance of these microorganisms in the environment are not available. Only a small percentage of bacteria colonizing a given ecosystem (0.3% in soil and <0.1% in water) can be identified with the use of conventional culture-based methods [23][24][25]. The precise determination of microbial abundance requires a combination of methods, which is laborious and time-consuming [26,27].…”

Section: Introductionmentioning

confidence: 99%

“…These probes target the 16S rRNA sequence in bacterial cells which are visualized under an epifluorescent microscope. The FISH technique opens new possibilities in research, and it supports real-time determination of the quantitative and qualitative composition, structure, and spatial distribution of bacterial populations in the environment with high precision and accuracy [24,25,34]. The FISH method is used in clinical studies to identify pathogenic microorganisms [35,36] and the microbiota present in soil [37], sediments, [38] and freshwater and marine water ecosystems [6,25].…”

Section: Introductionmentioning

confidence: 99%

“…The FISH technique opens new possibilities in research, and it supports real-time determination of the quantitative and qualitative composition, structure, and spatial distribution of bacterial populations in the environment with high precision and accuracy [24,25,34]. The FISH method is used in clinical studies to identify pathogenic microorganisms [35,36] and the microbiota present in soil [37], sediments, [38] and freshwater and marine water ecosystems [6,25]. This technique can also be used to study microbial metabolism because the target sites of hybridization are mainly intact ribosomes that are present in large numbers only in viable and active cells [24,39].…”

Section: Introductionmentioning

confidence: 99%

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The Importance of Enterococci in the Monitoring of Fecal Pollution in River Water in Forests and Urban Areas

Gotkowska-Płachta,

Gołaś

2023

Water

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The aim of this study was to determine the spatial distribution of enterococci as indicators of fecal pollution in river water in forest reserves and urban areas. The biodiversity, multidrug resistance, and virulence of enterococci were monitored using conventional and molecular methods, including fluorescence in situ hybridization (FISH). Enterococcal (ENT) counts determined via the FISH method were several thousand times higher than those determined by the culture-based method. This observation points to the abundance of viable but non-culturable (VBNC) bacteria that are often more toxic. Water from the river source was characterized by the lowest number of multidrug-resistant and virulent enterococcal strains. The abundance of the analyzed bacteria was highest (more than 50% of the strains) in the area where treated wastewater was evacuated to the river. Statistical analysis confirmed the presence of relationships between these environments. Pathogenic enterococci, which are not effectively eliminated during wastewater treatment, spread in the river continuum, thus posing a health threat to humans and animals. A combination of conventional and molecular techniques for the identification of bacteria supports a rapid and reliable assessment of pollution sources in the examined environment and the implementation of protective measures.

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