Fecal Calprotectin and Eosinophil‐derived Neurotoxin in Healthy Children Between 0 and 12 Years

Roca, María; Varela, Ana Rodriguez; Donat, Ester; Cano, Francisco; Hervás, David; Armisen, Ana; Vaya, Maria Jose; Sjölander, Anders; Ribes-Koninckx, Carmen

doi:10.1097/mpg.0000000000001542

Cited by 45 publications

(52 citation statements)

References 26 publications

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“…In our study, the mean EDN concentration in stool was significantly higher in the study group than in controls ( p <0.05), sensitivity reached 74.1%, specificity 80% with cut off value 884.45 ng/mL. The before mentioned study, by Roca et al, estimated the 95th percentile for EDN upon 7.4 mg/kg in infants, which remains in the agreement with our results [ 22 ].…”

Section: Discussionsupporting

confidence: 92%

“…Ezri et al indicated that fCal values are age-dependent and estimated cut-off levels upon <350 µg/g in the first year of life [ 21 ]. A recent study has presented the normogram for fCal concentration, in which the 95th percentile being 910.3 mg/kg for 0–12 months of age [ 22 ]. In our study, the median fCal value was 331.7 µg/g, 74–751 µg/g in 25 controls below 1 year of age, similar to Ezir et al results and significantly lower than the Roca et al study.…”

Section: Discussionmentioning

confidence: 99%

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Eosinophil-Derived Neurotoxin, Tumor Necrosis Factor Alpha, and Calprotectin as Non-Invasive Biomarkers of Food Protein-Induced Allergic Proctocolitis in Infants

Rycyk

Cudowska

Lebensztejn

2020

JCM

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Diagnosis of non-IgE mediated food allergy presents a special challenge due to lack of a single, non-invasive diagnostic method. We selected three fecal biomarkers of allergic inflammation of gastrointestinal origin in order to improve the diagnostic process. Twenty-seven infants with symptoms of hematochezia were prospectively enrolled into this study. All patients underwent a complete differential diagnosis of rectal bleeding. Non-IgE mediated food allergy was confirmed by an open, oral food challenge. The control group included twenty-five infants with functional gastrointestinal disorders. Eosinophil-derived neurotoxin (EDN), tumor necrosis factor alpha (TNFα), and calprotectin concentration were measured in stools of all children by enzyme-linked immunosorbent assays (ELISA) using commercial kits. Median eosinophil-derived neurotoxin and calprotectin fecal levels were significantly higher in the study group than in the control group (p < 0.05). The difference of fecal tumor necrosis factor alpha concentration between both groups was not statistically significant (p > 0.05). The best diagnostic performance was reached in a combination of fecal calprotectin (fCal) and EDN i.e., 88.9% and 84%, respectively. Fecal EDN and fCAl are reliable tools in differentiating between food protein-induced allergic proctocolitis and gastrointestinal functional disorders in infants.

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Section: Discussionsupporting

confidence: 92%

Section: Discussionmentioning

confidence: 99%

Eosinophil-Derived Neurotoxin, Tumor Necrosis Factor Alpha, and Calprotectin as Non-Invasive Biomarkers of Food Protein-Induced Allergic Proctocolitis in Infants

Rycyk

Cudowska

Lebensztejn

2020

JCM

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“…In healthy adult patients, FC concentrations < 50 µg/g faeces is considered to be normal, concentrations between 50 and 100 µg/g are treated as borderline while concentrations > 100 µg/g need more precise intervention and deeper clinical workup ( 13 ). Importantly, FC concentrations have been shown to vary with age, the highest concentrations reported in new-borns ( 14 – 17 ).…”

Section: Introductionmentioning

confidence: 99%

Analytical evaluation of a fully automated immunoassay for faecal calprotectin in a paediatric setting

et al. 2017

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IntroductionFaecal calprotectin (FC) is a routinely used marker for identifying and monitoring children with inflammatory bowel disease (IBD). This non-invasive test is useful for screening children with gastrointestinal symptoms to avoid unnecessary invasive procedures. In this study, we validated for the first time the performance of a fully automated particle-enhanced turbidimetric immunoassay (PETIA) on the VITROS® 5600 analyzer for measurement of FC in symptomatic children and adolescents.Materials and methodsFor performance validation of the PETIA (fCAL® turbo, Bühlmann Laboratories, Switzerland) on the VITROS® 5600 analyzer (Ortho Clinical Diagnostics, USA) limit of quantitation (LoQ), linearity, precision data and calibration curve stability were defined. Additionally, 95 faecal samples were measured using the PETIA, an enzyme-linked immunosorbent assay (ELISA; fCAL®, Bühlmann Laboratories, Switzerland) and a semi-quantitative lateral flow assay (Quantum Blue Reader®, Bühlmann Laboratories, Switzerland) for agreement evaluation.ResultsThe LoQ for calprotectin using PETIA on the VITROS® 5600 analyzer was 21 µg/g. The linearity range was 20 - 2100 µg/g and the precision study showed a total coefficient of variation (CV) between 2.3% and 8.9%. The calibration curve was stable for 4 weeks. Using the clinical samples quantifiable by PETIA, ELISA and the semi-quantitative lateral flow assay, Passing-Bablok regression analysis and Bland-Altman plots showed good agreement.ConclusionsDue to good performance characteristics and agreement with established methods, the fully automated PETIA on the routine chemistry analyzer VITROS® 5600 is a new analytical option for the rapid determination of FC.

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“…Oord e Hornung (2014) sugeriram 3 valores de ponto de corte para avaliar CLP em crianças saudáveis, baseados nos percentis 97,5%: 538 mg/kg (1 a 6 meses), 214 mg/kg (6 meses a 3 anos) e 75 mg/kg (3 a 4 anos). Roca et al (2017) também encontraram valores elevados de CLP nos primeiros anos de vida de crianças saudáveis (17,3 mg/kg a 2681,7 mg/kg ) e sugeriram valores de referência para a faixa pediátrica, considerando o percentil 95% para cada faixa etária: 910,3 mg/kg (0 a 12 meses), 285,9 mg/kg (1 a 4 anos) e 54,4 mg/kg ( 4 a 12 anos). No presente estudo, também foram observados valores maiores de CLP nos menores de 4 anos em relação às crianças saudáveis, porém sem diferença significante em relação aos maiores de 4 anos (p = 0,30), com médias e medianas superiores às descritas na literatura.…”

Section: Discussionunclassified

“…Apesar da sugestão de alguns autores em relação aos pontos de corte em diferentes grupos etários, os achados são muito variáveis entre os trabalhos, ainda não permitindo o estabelecimento de um ponto de corte para essas crianças. Isso sugere que a principal utilidade da CLP em menores de 4 anos, seria a monitorização da variação intraindividual, o que permitiria a avaliação da progressão da doença ou resposta a intervenções terapêuticas (ROCA et al, 2017).…”

Section: Discussionunclassified

Inflamação intestinal em crianças com fibrose cística

Ferreira¹

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Ao pessoal do Laboratório de Pediatria do HCRP, obrigada por darem todas as condições que permitiram a realização dos experimentos. Agradeço à equipe do Laboratório de Parasitologia do HCRP, que cederam o espaço para realização de parte das análises. Meu agradecimento especial ao Sr. Pedro, pela amizade, alegria, pelos ensinamentos em laboratório e pelo apoio constante, e à Luciana, por me acompanhar na realização dos experimentos, pela disponibilidade, paciência, amizade e pelos momentos de descontração. Vocês contribuíram enormemente para realização deste projeto. Às equipes da gastropediatria e do Ambulatório Multidisciplinar de Fibrose Cística, pelo apoio e incentivo ao meu projeto e pelas contribuições para realizá-lo. À FAEPA e ao Departamento de Puericultura e Pediatria da FMRP-USP, pelo apoio financeiro para a realização desta pesquisa. Aos meus professores da residência de pediatria da UFG, em especial Prof.

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Fecal Calprotectin and Eosinophil‐derived Neurotoxin in Healthy Children Between 0 and 12 Years

Cited by 45 publications

References 26 publications

Eosinophil-Derived Neurotoxin, Tumor Necrosis Factor Alpha, and Calprotectin as Non-Invasive Biomarkers of Food Protein-Induced Allergic Proctocolitis in Infants

Eosinophil-Derived Neurotoxin, Tumor Necrosis Factor Alpha, and Calprotectin as Non-Invasive Biomarkers of Food Protein-Induced Allergic Proctocolitis in Infants

Analytical evaluation of a fully automated immunoassay for faecal calprotectin in a paediatric setting

Inflamação intestinal em crianças com fibrose cística

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