Fecal Estrone Sulfate Profile of a Sow Showing Abnormal Pregnancy with Fetal Mummification.

Ohtaki, Tadatoshi; Moriyoshi, Masaharu; Nakada, Ken; Nakao, Toshihiko

doi:10.1292/jvms.62.309

Cited by 2 publications

(1 citation statement)

References 12 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The E 1 S equivalent concentrations in one sow were high at day 33 and 42 after mating, then remained low thereafter. This may be explained by fetal death or mummification later than 42 days after mating because in the sow with fetal mummification faecal E 1 S concentration was lower before farrowing than that in normal farrowing sows (Ohtaki et al. 2000).…”

Section: Discussionmentioning

confidence: 98%

Pregnancy Diagnosis in Miniature Pig by Direct ELISA of Oestrone Derivatives in Faeces

Isobe

Nakao

2004

Reprod Domestic Animals

View full text Add to dashboard Cite

The objectives of the present study were to measure oestrone derivatives [oestrone, oestrone sulphate (E1S) and oestrone glucuronide] in sow faeces by direct enzyme-linked immunosorbent assay (ELISA) and to explore the changes of oestrone derivative profile in faeces of miniature pig for demonstrating the possibility of pregnancy diagnosis. Faecal samples (1.5 g) were put into 6 ml of buffer, shaken and centrifuged. Then supernatant was added to the wells of multi-plate without extraction and incubated with anti-oestrone antibody and horseradish peroxidase-labelled oestrone. Standard solution was prepared at various concentrations of E1S. Sensitivity was estimated as 0.035 ng/ml (0.14 ng/g). Intra- and inter-assay coefficient variations were 3.5-7.7% and 10.9-15.3%, respectively. When 1-5 ng/ml E1S were added to a faecal solution, recovery rates ranged between 80.0 and 103.3%. There is a temporal increase in the E1S equivalent concentration of miniature pig faeces from day 25 to 31 after mating. From day 35 to 70, the E1S equivalent concentrations remained low. Thereafter its concentrations increased again towards farrowing. On day 27 and 29 after mating pregnancy diagnosis by the faecal E1S equivalent concentrations agreed with the results of farrowing (seven of seven animals). These results suggest that the present direct ELISA is practical and suitable as a routine assay for measuring the faecal concentration of oestrone derivatives and that this assay might be usable for pregnancy diagnosis in sows at day 27-29.

show abstract

Section: Discussionmentioning

confidence: 98%