Fecal flora measurements of breastfed infants using an integrated transport and culturing system

Fanaro, Silvia; Vigi, Vittorio; Chierici, R; Boehm, Günther

doi:10.1111/j.1651-2227.2003.tb02521.x

Cited by 32 publications

(28 citation statements)

References 5 publications

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“…Recently, metagenomic and microbiomic studies based on the analysis of genomic DNA-and rRNA gene libraries have highlighted the diversity of the GIT microbiota and have shown that it consists of many novel and, as yet, unculturable bacterial components (Eckburg et al, 2005;Wang et al, 2005;Gill et al, 2006;Kurokawa et al, 2007;Palmer et al, 2007). However, relatively few bifidobacterial rRNA gene sequences have so far been identified in these metagenomic studies, which are in striking contrast to what was described earlier in several bifidobacterial surveys, which had adopted culture-dependent approaches (Poxton et al, 1997;Harmsen et al, 2000;Fanaro et al, 2003).…”

contrasting

confidence: 46%

Microbiomic analysis of the bifidobacterial population in the human distal gut

et al. 2009

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One of the most complex microbial ecosystems is represented by the microbiota of the human gastrointestinal tract (GIT). Although this microbial consortium has been recognized to have a crucial effect on human health, its precise composition is still not fully established. Among the GIT bacteria, bifidobacteria represent an important commensal group whose presence is often associated with health-promoting effects. In this work, we assessed the complexity of the human intestinal bifidobacterial population by analysing the diversity of several 16S rRNA gene-based libraries. These analyses showed the presence of novel bifidobacterial phylotypes, which had not been found earlier and may thus represent novel taxa within the genus Bifidobacterium.

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contrasting

confidence: 46%

Microbiomic analysis of the bifidobacterial population in the human distal gut

et al. 2009

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“…Stool frequency, fecal pH and amount of produced acetic acid were also comparable in the breast-fed infants, indicating hat a low GOS formula has the same prebiotic effect as a high GOS formula, but a minimized risk of intestinal irritation. Breast-fed infants have an intestinal microflora dominated by Bifidobacteria and Lactobacilli [1,2] and are of the addition of GOS, and are, therefore, probably related to the changes in bacterial flora and fermentation. Studies in adults showed that a greater amount of dietary oligosaccharides may lead to adverse effects, flatulence in particular [23] .…”

Section: Discussionmentioning

confidence: 99%

“…Fecal samples were collected from a subpopulation (Tables 2 and 3) for analysis of intestinal bacteria (n = 82), short chain fatty acids (SCFAs) (n = 96) and pH (n = 112). For analysis of Bifidobacteria and Lactobacilli [1,12,13] , one gram of fresh feces was homogenized and diluted in 10 mL of a pre-reduced brain and heart infusion broth in an anaerobic glove box within 1 h of collection. Ten µL of each dilution was spread on the surfaces of Rogosa SL agar (Difco, Detroit, USA) dishes and incubated anaerobically at 37℃.…”

Section: Dietsmentioning

confidence: 99%

“…Breast milk is superior over artificial formula in many aspects, including its effect on the development of intestinal microflora. Breast-fed infants have a higher level of intestinal Bifidobacteria and Lactobacilli [1,2] , both of which are known to be potentially beneficial to the health of their hosts [3,4] . It is likely that oligosaccharides in human milk are more beneficial to intestinal flora [5][6][7] .…”

Section: Introductionmentioning

confidence: 99%

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Low level of galacto-oligosaccharide in infant formula stimulates growth of intestinal Bifidobacteria and Lactobacilli

Xiao-ming¹,

Li²,

Feng³

et al. 2008

WJG

137

125

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AIM:To investigate the effect of a new infant formula supplemented with a low level (0.24 g/100 mL) of galacto-oligosaccharide (GOS) on intestinal micro-flora (Bifidobacteria , Lactobacilli and E. coli ) and fermentation characteristics in term infants, compared with human milk and a standard infant formula without GOS. METHODS: Term infants (n = 371) were approached in this study in three hospitals of China. All infants started breast-feeding. Those who changed to formula-feeding within 4 wk after birth were randomly assigned to one of the two formula groups. Growth and stool characteristics, and side effects that occurred in recruited infants were recorded in a 3-mo follow-up period. Fecal samples were collected from a subpopulation of recruited infants for analysis of intestinal bacteria (culture technique), acetic acid (gas chromatography) and pH (indicator strip). RESULTS:After 3 mo, the intestinal Bifidobacteria , Lactobacilli , acetic acid and stool frequency were significantly increased, and fecal pH was decreased in infants fed with the GOS-formula or human milk, compared with those fed with the formula without GOS. No significant differences were observed between the GOS formula and human milk groups. Supplementation with GOS did not influence the incidence of crying, regurgitation and vomiting. CONCLUSION: A low level of GOS (0.24 g/100 mL) in infant formula can improve stool frequency, decrease fecal pH, and stimulate intestinal Bifidobacteria and Lactobacilli as in those fed with human milk.

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“…longum, which is thus indicative of vertical transmission (11,12). Mode of delivery (i.e., vaginally delivered versus delivered by cesarean section) and type of nutrition (i.e., breast-fed versus bottle-fed) are considered to be important factors that provide differential colonization opportunities, thereby impacting the composition of the neonatal gut microbiota, including the colonization level and species composition of bifidobacteria (13,14).…”

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confidence: 99%

Genomics of the Genus Bifidobacterium Reveals Species-Specific Adaptation to the Glycan-Rich Gut Environment

Milani

Turroni

Duranti

et al. 2016

Appl Environ Microbiol

187

136

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b Bifidobacteria represent one of the dominant microbial groups that occur in the gut of various animals, being particularly prevalent during the suckling period of humans and other mammals. Their ability to compete with other gut bacteria is largely attributed to their saccharolytic features. Comparative and functional genomic as well as transcriptomic analyses have revealed the genetic background that underpins the overall saccharolytic phenotype for each of the 47 bifidobacterial (sub)species representing the genus Bifidobacterium, while also generating insightful information regarding carbohydrate resource sharing and crossfeeding among bifidobacteria. The abundance of bifidobacterial saccharolytic features in human microbiomes supports the notion that metabolic accessibility to dietary and/or host-derived glycans is a potent evolutionary force that has shaped the bifidobacterial genome.

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Fecal flora measurements of breastfed infants using an integrated transport and culturing system

Cited by 32 publications

References 5 publications

Microbiomic analysis of the bifidobacterial population in the human distal gut

Microbiomic analysis of the bifidobacterial population in the human distal gut

Low level of galacto-oligosaccharide in infant formula stimulates growth of intestinal Bifidobacteria and Lactobacilli

Genomics of the Genus Bifidobacterium Reveals Species-Specific Adaptation to the Glycan-Rich Gut Environment

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