Fecal imaging demonstrates that low-methoxyl pectin supplementation normalizes gastro-intestinal transit in mice given a liquid diet

Kagawa, Tomohiro; Endo, Naoyuki; Ebisu, Goro; Yamaoka, Ippei

doi:10.14814/phy2.13662

Cited by 8 publications

(4 citation statements)

References 23 publications

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“…Acute loperamide administration indeed slowed whole gut transit and decreased the number of produced pellets. Interestingly, we found that our control transit group had slower whole gut transit times than many reported in the literature, 11‐18 and we noted high pellet output in the early time points. Thus, we hypothesized that the faster transit times reported elsewhere might be due to the disruption of animal behavior during measurements.…”

Section: Discussionsupporting

confidence: 45%

A simple automated approach to measure mouse whole gut transit

Kacmaz

Alto

Knutson

et al. 2020

Neurogastroenterology Motil

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Background Gastrointestinal (GI) motility is a complex physiological process that is critical for normal GI function. Disruption of GI motility frequently occurs in GI diseases or as side effects of therapeutics. Whole gut transit measurements, like carmine red leading‐edge transit, in mice form the cornerstone of in vivo preclinical GI motility studies. Method We have developed an easily achievable, labor‐saving method to measure whole gut transit time in mice. This approach uses inexpensive, commercially available materials to monitor pellet production over time via high definition cameras capturing time‐lapse video for offline analysis. Key Result We describe the assembly of our automated gut transit setup and validate this approach by comparing the results with loperamide to delay transit and conventional transit measurements. We demonstrate that compared to the control group, the loperamide group had slowed transit, evidenced by a decrease in total pellet production and prolonged whole gut transit time. The control group had an extended transit time compared with the results reported in the literature. Whole gut transit rates accelerated to times comparable to the literature by disrupting cages every 10‐15 min to imitate the conventional approach, suggesting that disruption affects the assay and supports the use of an automated approach. Conclusion & Inferences A novel automated, inexpensive, and easily assembled whole gut transit setup is labor‐saving and allows minimal disruption to animal behavior compared with the conventional approach.

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Section: Discussionsupporting

confidence: 45%

A simple automated approach to measure mouse whole gut transit

Kacmaz

Alto

Knutson

et al. 2020

Neurogastroenterology Motil

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“…In rodents, GI transit is typically monitored by the visual observation of the first appearance of the marker in the feces or by measuring the distance traveled by a tracer into the gastrointestinal segments after the death of the animals . Whole‐gut transit evaluated by the dye method from oral gavage was 130 minutes for BALB/c and 100‐140 minutes for C57BL/6 . In our in vivo study, BALB/c and C57BL/6 strains had OATT of 96 and 150 minutes, respectively.…”

Section: Discussionmentioning

confidence: 99%

Gastrointestinal motility and morphology in mice: Strain‐dependent differences

Gama

Machado

Beckmann

et al. 2020

Neurogastroenterology Motil

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Background: BALB/c and C57BL/6 mice are widely used in biomedical research; however, the differences between strains are still underestimated. Our aims were to develop an experimental protocol to evaluate the duodenal contractility and gastrointestinal transit in mice using the Alternating Current Biosusceptometry (ACB) technique and to compare gastrointestinal motor function and morphology between BALB/c and C57BL/6 strains. Methods: Male mice were used in experiments (a) duodenal contractility: animals which had a magnetic marker surgically fixed in the duodenum to determine the frequency and amplitude of contractions and (b) gastrointestinal transit: animals which ingested a magnetically marked chow to calculate the Oro-Anal Transit Time (OATT) and the Fecal Pellet Elimination Rate (FPER). The animals were killed after the experiments for organ collection and morphometric analysis.Key Results: BALB/c and C57BL/6 had two different duodenal frequencies (high and low) with similar amplitudes. After 10 hours of monitoring, BALB/c eliminated around 89% of the ingested marker and C57BL/6 eliminated 33%; OATT and FPER were slower for C57BL/6 compared with BALB/c. The OATT and amplitude of low frequency had a strong positive correlation in C57BL/6. For BALB/c, the gastric muscular layer was thicker compared to that measured for C57BL/6. Conclusions and Inferences:The experimental protocol to evaluate duodenal contractility and fecal magnetic pellets output using the ACB technique in mice was successfully established. BALB/c strains had higher duodenal frequencies and a shorter time to eliminate the ingested marker. Our results showed differences in both motor function and gastrointestinal morphology between BALB/c and C57BL/6 strains. K E Y W O R D S BALB/c, C57BL/6, duodenal contractility, gastrointestinal transit, inbred S U PP O RTI N G I N FO R M ATI O N Additional supporting information may be found online in the Supporting Information section.

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“…Transfecting various cancer cell lines using OATP1B3 or NTCP further verified these findings [ 15 16 17 ]. Although ICG was transported through transporters similar to bilirubin, findings indicated that intestinal bilirubin transporters are not responsible for the transportation of ICG [ 18 ].…”

Section: B Iochemical and Physical Properties Of Indocyanine Greenmentioning

confidence: 99%

Indocyanine green

Hsiao

2021

Tzu Chi Medical Journal

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Indocyanine green (ICG), a US Food and Drug Administration-approved fluorescent compound, has been on the medical stage for more than 60 years. Current uses include hepatic function evaluation before surgical procedure and fundus evaluation. The large safety margin and near-infrared fluorescent optical advantage of the drug have proved useful in several clinical trials of intraoperative systems for tumor removal. Several nanoparticle-sized formulations for thermal ablation and photodynamic therapy have also been evaluated in animal experiments. Studies have attempted to manipulate ICG as a reporter fluorophore with initial success. In this article, we reviewed ICG's histological applications, chemical and physical properties, current clinical applications, ongoing clinical trials, and biomedical studies and prospects. We believe that ICG could be used with novel biotechnological techniques, such as fluorescent endoscopy and photoacoustic equipment, in a range of biomedical fields.

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Fecal imaging demonstrates that low-methoxyl pectin supplementation normalizes gastro-intestinal transit in mice given a liquid diet

Cited by 8 publications

References 23 publications

A simple automated approach to measure mouse whole gut transit

A simple automated approach to measure mouse whole gut transit

Gastrointestinal motility and morphology in mice: Strain‐dependent differences

Indocyanine green

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