Fecal microbiota transplantation confers beneficial metabolic effects of diet and exercise on diet-induced obese mice

Abstract

Diet and exercise are conventional methods for controlling body weight and are linked to alterations in gut microbiota. However, the associations of diet, exercise, and gut microbiota in the control of obesity remain largely unknown. In the present study, using 16S rRNA amplicon sequencing and fecal microbiota transplantation (FMT), normal fat diet (NFD), exercise and their combination resulted in improved metabolic profiles in comparison to sedentary lifestyle with high fat diet (HFD). Moreover, diet exerted more influence than exercise in shaping the gut microbiota. HFD-fed mice receiving FMT from NFD-exercised donors not only showed remarkably reduced food efficacy, but also mitigated metabolic profiles (p < 0.05). The transmissible beneficial effects of FMT were associated with bacterial genera Helicobacter, Odoribacter and AF12 and overrepresentation of oxidative phosphorylation and glycolysis genes. Our findings demonstrate that the beneficial effects of diet and exercise are transmissible via FMT, suggesting a potential therapeutic treatment for obesity.

Physical activity shapes the intestinal microbiome and immunity of healthy mice but has no protective effects against colitis in MUC2-/- mice

“…Others have also reported that elite athletes have higher alpha diversity than sedentary controls (92), or that exercise training in mice leads to increased Shannon diversity (93,94). However, in agreement with the current experiment, PA has been shown to have no effect on alpha diversity in mice (29,95,96), rats (97), and humans (30,98). The reasons for these discrepancies are not clear, though multiple factors such as differences in animal vendors and facilities, DNA extraction methods and sequencing, bioinformatics analysis, and statistical testing methods, are likely involved.…”

Review: Other Helicobacter species

“…61 Mice lacking fat mass-and the obesity-associated gene (Fto) exhibited reduced anxiety-and depression-like behavior, as well as a specific bacterial signature that suppresses inflammation, characterized by a lower abundance of Porphyromonadaceae and Helicobacter and a higher abundance of Lactobacilli. 63 Furthermore, the gut microbiota mediates the anti-obesity effect of calorie restriction in mice. 63 Furthermore, the gut microbiota mediates the anti-obesity effect of calorie restriction in mice.…”

“…62 Fecal microbiota transplantation (FMT) has been reported to confer the beneficial metabolic effects of diet and exercise on diet-induced obese mice; these effects have been associated with bacterial genera including Helicobacters, in particular Helicobacter typhlonius, thus reflecting the genus vulnerability to diet, exercise, and even FMT. 63 Furthermore, the gut microbiota mediates the anti-obesity effect of calorie restriction in mice. 64 Indeed, calorie restriction has been shown to alter the composition of the gut microbiota with significant increases in probiotic genera and a decrease in harmful bacteria such as Helicobacters.…”

Obesity-induced gut microbiota dysbiosis can be ameliorated by fecal microbiota transplantation: a multiomics approach

“…Fecal microbiota transplantation (FMT) is a new and straightforward therapy that manipulates the gut microbiota by transferring healthy donor microbiota into an existing disrupted gut microbial ecosystem. This therapy can be an effective approach to obesity treatment [12,13]. Even though FMT has some limitations, several studies have tested its effectiveness and have demonstrated an improvement of some comorbidities associated not only with obesity [14] but also with other noncommunicable diseases [15–18].…”