Fecal Microbiota Transplantation Donor and Dietary Fiber Intervention Collectively Contribute to Gut Health in a Mouse Model

Abstract

Transforming the gut microbiota has turned into the most intriguing target for interventions in multiple gastrointestinal and non-gastrointestinal disorders. Fecal microbiota transplantation (FMT) is a therapeutic tool that administers feces collected from healthy donors into patients to help replenish the gut microbial balance. Considering the random donor selection, to maintain the optimal microbial ecosystem, post-FMT is critical for therapy outcomes but challenging. Aiming to study the interventions of different diets on recipients’ gut microbiota post-FMT that originated from donors with different diets, we performed FMT from domestic vs. wild pigs that are living on low-fiber vs. high-fiber diets into the pseudo-GF mouse, followed with fiber-free (FF) or fiber-rich (FR) diets post-FMT. Different patterns of gut microbiota and metabolites were observed when mice FMT from different donors were paired with different dietary fiber contents. Enrichment of bacteria, including Akkermansia and Parabacteroides, together with alteration of metabolites, including palmitic acid, stearic acid, and nicotinic acid, was noted to improve crypt length and mucus layer in the gut in mice FMT from wild pigs fed an FR diet. The results provide novel insight into the different responses of reconstructed gut microbiota by FMT to dietary fiber. Our study highlighted the importance of post-FMT precise dietary interventions.

Characterization and diagnostic value of the gut microbial composition in patients with minimal change disease

“…Fecal microbiota transplantation (FMT) has been widely utilized in researches involving animal models ( Daniel et al, 2022 ; Zhong et al, 2022 ). We also performed FMT on MCD rat model to explore the therapeutic or aggravative effects of gut microbiota.…”

Effects of Dietary Microcapsule Sustained-Release Sodium Butyrate on the Growth Performance, Immunity, and Gut Microbiota of Yellow Broilers

“…The SCFAs including the acetate, propionate, isobutyrate, butyrate, isovalerate, and valerate in cecum digesta were quantified by gas chromatography [22]. In brief, 0.5 g of cecal contents was taken into the centrifuge tube, combined with ddH 2 O, and centrifuged.…”

Fecal virus transplantation has more moderate effect than fecal microbiota transplantation on changing gut microbial structure in broiler chickens

“…Studies have demonstrated that both microbes and phages can affect the tight junction protein between intestinal epithelial cells and regulate intestinal permeability under pathological conditions ( Zhong et al, 2022 ). However, it remains unclear the effect of transplantation from 28-day-old AA broiler cecum microorganisms into healthy broiler chickens on the jejunal barrier function, digestion and absorption.…”