Fecal microbiota transplantation improves metabolism and gut microbiome composition in db/db mice

Abstract

Fecal microbiota transplantation (FMT) has become an effective strategy to treat metabolic diseases, including type 2 diabetes mellitus (T2DM). We previously reported that the intestinal microbiome had significant difference between individuals with normal glucose tolerance and T2DM in Chinese Kazak ethnic group. In this study, we investigated the effects of transplanted fecal bacteria from Kazaks with normal glucose tolerance (KNGT) in db/db mice. The mice were treated with 0.2 mL of fecal bacteria solution from KNGT daily for 10 weeks. We showed that the fecal bacteria from KNGT successfully colonized in the intestinal tract of db/db mice detected on day 14. In the FMT-treated db/db mice, the levels of fasting blood glucose, postprandial glucose, total cholesterol, triglyceride, and low-density lipoprotein–cholesterol were significantly downregulated, whereas high-density lipoprotein–cholesterol levels were upregulated. In the FMT-treated db/db mice, Desulfovibrio and Clostridium coccoides levels in gut were significantly decreased, but the fecal levels of Akkermansia muciniphila and colon histone deacetylase-3 (HDAC3) protein expression were increased. At 8 weeks, both intestinal target bacteria and HDAC3 were correlated with glycolipid levels; Akkermansia muciniphila level was positively correlated with HDAC3 protein expression ( r = +0.620, P = 0.037). Our results suggest that fecal bacteria from KNGT could potentially be used to treat diabetic patients.

Fecal transplantation and butyrate improve neuropathic pain, modify immune cell profile, and gene expression in the PNS of obese mice

“…S2B). However, in our model and as published by many authors (38)(39)(40), fasting glucose was slightly lower after FMT in comparison to sham FMT, but significantly lower when compared to WD-fed, antibiotics-treated, S-gavaged mice that did not receive DS (two-way ANOVA showed a main effect of FMT gavage # P = 0.0042; Sidak post hoc, *P = 0.04) (SI Appendix, Fig. S2C).…”

Modulating the Microbiota as a Therapeutic Intervention for Type 2 Diabetes

“…In rodent models, insulin sensitivity significantly improved after transferring microbiota in MyD88 deficient NOD mice ( 109 ). Similar studies where human microbiota from healthy Chinese subjects are transplanted into diabetic db/db mice remarkably lowers fasting blood glucose concentrations ( 110 ). Likewise, transplantation of fecal samples of patients treated with metformin into germ-free mice improves glucose tolerance ( 111 ).…”

TMAO: how gut microbiota contributes to heart failure

“…121 Some parenteral diseases are also being explored, such as autism, 122 obesity, 123 and type 2 diabetes. 124 Vrieze et al 125 performed FMT treatment on patients with metabolic syndrome, and the result indicated that patients receiving gut flora from leptin donor displayed improved insulin sensitivity and increased butyrate production. This finding was validated in another RCT, suggesting that FMT might be a potential treatment strategy for metabolism-related diseases.…”