Fecal transplantation for treatment of inflammatory bowel disease

Imdad, Aamer; Pandit, Natasha; Zaman, Muizz; Minkoff, Nathan Zev; Tanner‐Smith, Emily E.; Gómez‐Duarte, Oscar G.; Acra, Sari; Nicholson, Maribeth R

doi:10.1002/14651858.cd012774.pub3

Cited by 18 publications

(18 citation statements)

References 145 publications

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“…Disordered intestinal microbiota is the underlying pathogenic mechanism of IBD ( 28 – 30 ). To investigate whether intestinal flora is involved in H. pylori -alleviated colitis, we collected stool samples from these mice and subjected them to high-throughput shotgun metagenomic sequencing to profile their microbial community taxonomic composition.…”

Section: Resultsmentioning

confidence: 99%

Dual RNA sequencing of Helicobacter pylori and host cell transcriptomes reveals ontologically distinct host-pathogen interaction

Hu,

Zhai,

Huang

et al. 2024

mSystems

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Helicobacter pylori is a highly successful pathogen that poses a substantial threat to human health. However, the dynamic interaction between H. pylori and the human gastric epithelium has not been fully investigated. In this study, using dual RNA sequencing technology, we characterized a cytotoxin-associated gene A ( cagA )-modulated bacterial adaption strategy by enhancing the expression of ATP-binding cassette transporter-related genes, metQ and HP_0888 , upon coculturing with human gastric epithelial cells. We observed a general repression of electron transport-associated genes by cagA , leading to the activation of oxidative phosphorylation. Temporal profiling of host mRNA signatures revealed the downregulation of multiple splicing regulators due to bacterial infection, resulting in aberrant pre-mRNA splicing of functional genes involved in the cell cycle process in response to H. pylori infection. Moreover, we demonstrated a protective effect of gastric H. pylori colonization against chronic dextran sulfate sodium (DSS)-induced colitis. Mechanistically, we identified a cluster of propionic and butyric acid-producing bacteria, Muribaculaceae , selectively enriched in the colons of H. pylori -pre-colonized mice, which may contribute to the restoration of intestinal barrier function damaged by DSS treatment. Collectively, this study presents the first dual-transcriptome analysis of H. pylori during its dynamic interaction with gastric epithelial cells and provides new insights into strategies through which H. pylori promotes infection and pathogenesis in the human gastric epithelium. IMPORTANCE Simultaneous profiling of the dynamic interaction between Helicobacter pylori and the human gastric epithelium represents a novel strategy for identifying regulatory responses that drive pathogenesis. This study presents the first dual-transcriptome analysis of H. pylori when cocultured with gastric epithelial cells, revealing a bacterial adaptation strategy and a general repression of electron transportation-associated genes, both of which were modulated by cytotoxin-associated gene A ( cagA ). Temporal profiling of host mRNA signatures dissected the aberrant pre-mRNA splicing of functional genes involved in the cell cycle process in response to H. pylori infection. We demonstrated a protective effect of gastric H. pylori colonization against chronic DSS-induced colitis through both in vitro and in vivo experiments. These findings significantly enhance our understanding of how H. pylori promotes infection and pathogenesis in the human gastric epithelium and provide evidence to identify targets for antimicrobial therapies.

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Section: Resultsmentioning

confidence: 99%

Dual RNA sequencing of Helicobacter pylori and host cell transcriptomes reveals ontologically distinct host-pathogen interaction

Hu,

Zhai,

Huang

et al. 2024

mSystems

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“…Five studies also evaluated the endpoint of endoscopic remission and demonstrated an odds-ratio of endoscopic remission of 1.45 as compared to controls (CI = 0.64-3.29). The risk of any adverse events was not increased in the FMT groups [53]. The authors conclude that "FMT may increase the proportion of people with active UC who achieve clinical and endoscopic remission" [53].…”

Section: Clinical Trials With Fmt In Ucmentioning

confidence: 92%

“…Induction of clinical remission was the endpoint and 10 of those studies. The meta-analysis indicates that FMT increases the rates of induction of clinical remission in UC patients as compared to placebo or sham-control with an odds ratio of 1.79 (CI: 1.13-2.84) [53]. Five studies also evaluated the endpoint of endoscopic remission and demonstrated an odds-ratio of endoscopic remission of 1.45 as compared to controls (CI = 0.64-3.29).…”

Section: Clinical Trials With Fmt In Ucmentioning

confidence: 99%

“…In the meantime, a number of additional clinical trials have been published and over the years several meta-analysis came to the conclusion that there is a significant positive effect of fecal microbiota transplantation in UC. A recent Cochrane meta-analysis looked into 12 studies on FMT in 550 UC patients [53]. In the 12 studies analyzed there was no homogeneous route of administration of FMT.…”

Section: Clinical Trials With Fmt In Ucmentioning

confidence: 99%

“…The risk of any adverse events was not increased in the FMT groups [53]. The authors conclude that "FMT may increase the proportion of people with active UC who achieve clinical and endoscopic remission" [53]. A small study (prematurely terminated due to COVID 19) revealed promising results in 35 patients with active UC using an oral lyophilized microbiota preparation and indicated that such as preparation may be a feasible way to achieve induction of remission in UC subsequent to antibiotic treatment [54].…”

Section: Clinical Trials With Fmt In Ucmentioning

confidence: 99%

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Microbiota Therapy in Inflammatory Bowel Disease

Biedermann,

Kreienbühl,

Rogler

2024

Visc Med

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Background: In both Crohn’s disease (CD) and ulcerative colitis (UC), the two major forms of inflammatory bowel disease (IBD) the immune reaction is – at least partially – directed against components of the luminal microbiota of the gut. These immune responses as well as other factors contribute to a phenomenon frequently described as “dysbiosis” meaning an alteration of the composition of the colonic microbiota. To improve the dysbiosis and to restore the normal composition of the colonic microbiota, fecal microbiota transplantation (FMT) has been tested as a therapeutic option to induce and maintain remission in IBD patients. Summary: This review will first discuss changes in the composition of the intestinal microbiota found in IBD patients and second the therapeutic potential of microbiological interventions for the treatment of these patients. FMT has been studied in several clinical trials in both, CD and UC. Reported results and subsequent meta-analyses indicate that FMT may be effective to induce remission in UC. However, the optimal route of FMT, the necessary number of administrations and the question whether life bacteria of freshly prepared stool is more effective than frozen are still unclear. Concepts associated with an optimization of FMT such as the “super donor concept” or the “consortia-approach” will be discussed to illustrate open questions and difficulties associated with microbiota therapy in IBD. Key Messages: The microbiota composition in IBD patients shows significant alterations compared to healthy individuals termed as “dysbiosis”. FMT and other therapeutic approaches to modify the microbiota composition have been studied in clinical trials in recent years. Efficacy has been shown in UC; however, many questions with respect to the optimization of microbiota therapy remain to be answered.

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What are the effects of fecal transplantation for people with ulcerative colitis?

Vedamurthy¹

2020

Cochrane Clinical Answers

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Fecal transplantation for treatment of inflammatory bowel disease

Cited by 18 publications

References 145 publications

Dual RNA sequencing of Helicobacter pylori and host cell transcriptomes reveals ontologically distinct host-pathogen interaction

Dual RNA sequencing of Helicobacter pylori and host cell transcriptomes reveals ontologically distinct host-pathogen interaction

Microbiota Therapy in Inflammatory Bowel Disease

What are the effects of fecal transplantation for people with ulcerative colitis?

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