Fecal Volatile Organic Compound Profiles are Not Influenced by Gestational Age and Mode of Delivery: A Longitudinal Multicenter Cohort Study

Deianova, Nancy; Hassani, Sofia el Manouni el; Niemarkt, Hendrik J.; Cossey, Veerle; Kaam, Anton H. van; Jenken, Floor; Weissenbruch, Mirjam M. van; Doedes, Esmee M; Baelde, Kyra; Menezes, Renée X. de; Benninga, Marc A.; Jonge, Wouter J. de; Boer, Nanne K de; Meij, Tim G. de

doi:10.3390/bios10050050

Cited by 12 publications

(13 citation statements)

References 62 publications

(88 reference statements)

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“…Given the increased abundance of the causative species in cases versus controls and our modeling demonstrating community structure differences between BSI from specific species, gut microbiome profiling could be used to ascribe late-onset sepsis risk to each infant. This finding complements data that show that longitudinal stool volatile profiling could offer late-onset sepsis predictions before onset ( 50 ). The possibility of performing rapid sequencing at the start of late-onset sepsis could also help inform antibiotic stewardship and direct more rapid, effective treatment of a causative organism ( 51 ).…”

Section: Discussionsupporting

confidence: 86%

Gut pathogen colonization precedes bloodstream infection in the neonatal intensive care unit

et al. 2023

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Bacterial bloodstream infections (BSIs) resulting in late-onset sepsis affect up to half of extremely preterm infants and have substantial morbidity and mortality. Bacterial species associated with BSIs in neonatal intensive care units (NICUs) commonly colonize the preterm infant gut microbiome. Accordingly, we hypothesized that the gut microbiome is a reservoir of BSI-causing pathogenic strains that increase in abundance before BSI onset. We analyzed 550 previously published fecal metagenomes from 115 hospitalized neonates and found that recent ampicillin, gentamicin, or vancomycin exposure was associated with increased abundance of Enterobacteriaceae and Enterococcaceae in infant guts. We then performed shotgun metagenomic sequencing on 462 longitudinal fecal samples from 19 preterm infants (cases) with BSI and 37 non-BSI controls, along with whole-genome sequencing of the BSI isolates. Infants with BSI caused by Enterobacteriaceae were more likely than infants with BSI caused by other organisms to have had ampicillin, gentamicin, or vancomycin exposure in the 10 days before BSI. Relative to controls, gut microbiomes of cases had increased relative abundance of the BSI-causing species and clustered by Bray-Curtis dissimilarity according to BSI pathogen. We demonstrated that 11 of 19 (58%) of gut microbiomes before BSI, and 15 of 19 (79%) of gut microbiomes at any time, harbored the BSI isolate with fewer than 20 genomic substitutions. Last, BSI strains from the Enterobacteriaceae and Enterococcaceae families were detected in multiple infants, indicating BSI-strain transmission. Our findings support future studies to evaluate BSI risk prediction strategies based on gut microbiome abundance in hospitalized preterm infants.

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Section: Discussionsupporting

confidence: 86%

Gut pathogen colonization precedes bloodstream infection in the neonatal intensive care unit

et al. 2023

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“…A recent study has showed that VOC profiles, as measured by an eNose device, in preterm infants born at GA <30 weeks, are not influenced by GA or mode of delivery during the first 3 weeks of life ( 36 ). In this study, we evaluated the effects of mode of delivery, birth weight, gender, and type of feeding on intestinal gases.…”

Section: Discussionmentioning

confidence: 99%

Evolution of Intestinal Gases and Fecal Short-Chain Fatty Acids Produced in vitro by Preterm Infant Gut Microbiota During the First 4 Weeks of Life

Wang

et al. 2021

Front. Pediatr.

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Background: The production of intestinal gases and fecal short-chain fatty acids (SCFAs) by infant gut microbiota may have a significant impact on their health, but information about the composition and volume of intestinal gases and SCFA profiles in preterm infants is scarce.Objective: This study examined the change of the composition and volume of intestinal gases and SCFA profiles produced by preterm infant gut microbiota in vitro during the first 4 weeks of life.Methods: Fecal samples were obtained at five time points (within 3 days, 1 week, 2 weeks, 3 weeks, and 4 weeks) from 19 preterm infants hospitalized in the neonatal intensive care unit (NICU) of Shanghai Children's Hospital, Shanghai Jiao Tong University between May and July 2020. These samples were initially inoculated into four different media containing lactose (LAT), fructooligosaccharide (FOS), 2′-fucosyllactose (FL-2), and galactooligosaccharide (GOS) and thereafter fermented for 24 h under conditions mimicking those of the large intestine at 37.8°C under anaerobic conditions. The volume of total intestinal gases and the concentrations of individual carbon dioxide (CO2), hydrogen (H2), methane (CH4), and hydrogen sulfide (H2S) were measured by a gas analyzer. The concentrations of total SCFAs, individual acetic acid, propanoic acid, butyric acid, isobutyric acid, pentanoic acid, and valeric acid were measured by gas chromatography (GC).Results: The total volume of intestinal gases (ranging from 0.01 to 1.64 ml in medium with LAT; 0–1.42 ml with GOS; 0–0.91 ml with FOS; and 0–0.44 ml with FL-2) and the concentrations of CO2, H2, H2S, and all six fecal SCFAs increased with age (p-trends < 0.05). Among them, CO2 was usually the predominant intestinal gas, and acetic acid was usually the predominant SCFA. When stratified by birth weight (<1,500 and ≥1,500 g), gender, and delivery mode, the concentration of CO2 was more pronounced among infants whose weight was ≥1,500 g than among those whose weight was <1,500 g (p-trends < 0.05).Conclusions: Our findings suggested that the intestinal gases and SCFAs produced by preterm infant gut microbiota in vitro increased with age during the first 4 weeks of life.

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“…Deianova et al [ 4 ] conducted an initial study to investigate the influence of gestational age (GA) and mode of delivery as potential factors affecting fecal VOC patterns in preterm infants born before 30 weeks of gestation. They utilized patients from a multicenter cohort study in nine participating neonatal intensive care units (NICUs) in the Netherlands and Belgium.…”

Section: Review Of Special Issue Contributionsmentioning

confidence: 99%

Noninvasive Early Disease Diagnosis by Electronic-Nose and Related VOC-Detection Devices

Wilson

2020

Biosensors

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This editorial provides an overview and summary of recent research articles published in Biosensors journal, volumes 9 (2019) and 10 (2020), within the Special Issue “Noninvasive Early Disease Diagnosis”, which focused on recent sensors, biosensors, and clinical instruments developed for the noninvasive early detection and diagnosis of human, animal, and plant diseases or invasive pests. The six research articles included in this Special Issue provide examples of some of the latest electronic-nose (e-nose) and related volatile organic compound (VOC)-detection technologies, which are being tested and developed to improve the effectiveness and efficiency of innovative diagnostic methodologies for the early detection of particular diseases and pest infestations in living hosts, prior to symptom development.

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Fecal Volatile Organic Compound Profiles are Not Influenced by Gestational Age and Mode of Delivery: A Longitudinal Multicenter Cohort Study

Cited by 12 publications

References 62 publications

Gut pathogen colonization precedes bloodstream infection in the neonatal intensive care unit

Gut pathogen colonization precedes bloodstream infection in the neonatal intensive care unit

Evolution of Intestinal Gases and Fecal Short-Chain Fatty Acids Produced in vitro by Preterm Infant Gut Microbiota During the First 4 Weeks of Life

Noninvasive Early Disease Diagnosis by Electronic-Nose and Related VOC-Detection Devices

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