Fed-batch culture of insect cells: a method to increase the yield of recombinant human nerve growth factor (rhNGF) in the baculovirus expression system

Comparison ofTrichoplusia ni BTI-Tn-5B1-4 (high five™) andSpodoptera frugiperda Sf-9 insect cell line metabolism in suspension cultures

“…Wang et al (1993a) found that glutamine and glucose addition resulted in enhanced cell growth and recombinant protein productivity. Other researchers found that yeastolate ultrafiltrate addition was required along with the addition of glucose (Drews et al, 1995), an amino acid mixture (Bedard et al, 1994), or glucose and glutamine (Nguyen et al, 1993;Reuveny et al, 1993b) in order to significantly enhance cell growth and recombinant protein productivity. Drews et al (1995), however, also demonstrated that high yeastolate ultrafiltrate concentrations inhibited cell growth.…”

“…Many previous studies have been conducted in which nutrients were added a limited number of times (usually only once) over the course of the Sf-9 cell culture (Bedard et al, 1994;Drews et al, 1995;Nguyen et al, 1993;Reuveny et al, 1993b;Wang et al, 1993a). Wang et al (1993a) found that glutamine and glucose addition resulted in enhanced cell growth and recombinant protein productivity.…”

Effect of elevated oxygen and glutamine levels on foreign protein production at high cell densities using the insect cell-baculovirus expression system

“…Glucose and glutamine are the primary energy sources for Spodoptera frugiperda cells and their concentrations have been manipulated post-infection to improve protein yields at high cell densities (Nguyen et al, 1993;. Glucose and glutamine disappearance from the medium (assumed to be due to cellular uptake) are shown in the top two panels of Figure 3 for four different cell densities.…”

Growth, metabolism and baculovirus production in suspension cultures of an Anticarsia gemmatalis cell line

“…Moreover, the metabolic characteristics of a cell line can determine the selection of a particular cultivation strategy, such as batch, fed-batch or perfusion. As an example, the lack of production of toxic by-products in well-oxygenated cultures of the lepidopteran insect cell lines IPLB-Sf-21 and Sf9 makes it possible to use nutrient feeding strategies in order to keep cultures in production at very high cell densities (Nguyen et al 1993;Bé dard et al 1997;Elias et al 2000). On the contrary, Tn-5 and High-Five cell lines display a metabolic behavior characterized by the accumulation of lactate and ammonia (Rhiel et al 1997) that could limit the applicability of nutrient feeding strategies in high density cultures of these cell lines (Ikonomou et al 2003).…”