Feed Additive Residues, Determination of 3,5-Dinitro-o-toluamide (Zoalene) in Chicken Tissues

Coccidiostat Identification, Methods of Distinguishing Zoalene and Its Metabolite from Other Coccidiostats

“…A series of 50-gram samples of untreated chicken muscle tissue to which 25 ^g. of 3,5-dinitro-o-toluamide had been added was analyzed for the compound by the method of Smith et al (6).…”

“…per ton). The chickens were sacrificed while still on medicated feed and 50-gram tissue samples were analyzed for zoalene by the method of Smith et al (6). Absorbance readings were made at 540, 560, and 600 µ and the ratios were calculated.…”

Pesticides

“…Practical applications of gas chromatography are stressed, but enough theoretical background is given to meet the requirements of the average analyst. Goodwin, Goulden, and Reynolds (81) have developed a rapid procedure for identification and quantitative determination of residues of chlorinated hydrocarbon pesticides in crops. In their rapid sorting test they used a 2foot column packed with 2.5% of E301 silicone elastomer and 0.25% of Epikote 1001 on 100-to 120-mesh kieselguhr.…”

Feed Additive Residues, Determination of 3-Amino-5-nitro-o-toluamide (ANOT) in Chicken Tissues

“…The average recovery from muscle tissue, over the range of 0.1 to 1.0 p.p.m., was 86 ±5% and from liver tissue, over the range of 0.25 to 4.0 p.p.m., was 87 =>=5%. When zoalene (3,5-dinitroo-toluamide) is fed to chickens, both the original compound and a metabolite, 3 -amino -5 -nitrootoluamide (ANOT), are found in the tissues [3,4). This paper describes the method for determining ANOT in chicken tissue.…”