Feed Additives Detection, Identification of 3,5-Dinitro-o-Toluamide (Zoalene) and Possible Metabolites by Paper Chromatography

Abstract

approximately 3.3 p.p.m. was bound. After a 24-hour withdrawal period, the radioactivity remaining in the liver tissue was apparently all bound compound.Similar results were obtained with muscle tissue using a scintillation counting technique which permitted detection of lower concentrations of radioactivity (5). The majority of the extractable material appeared to disappear very rapidly so that within 24 hours the majority of it had disappeared from the tissue (1.4 to 0.2 p.p.m.). The nonextractable activity decreased slightly (0.3 to 0.1 p.p.m.) during the first 24 hours and then remained more or less constant during the remaining portion of the observation period.Although the acetone extraction does not preparing the carboxyl-labeled 3,5-dinitro-o-toluamide-C14 used in these studies.

Chromatography

“…Partition chromatography on columns was only used in two instances: the determination of barbiturates (1708) and of amphetamine (1074). New paper-chromatographic techniques have been described for various narcotics (542,893,1196,1534,1649), barbiturates (161, 315, 362, 406, 650, 833-835, 900, 1125, 1237, 1515, 1531, 1742) and other sedatives (216,392,409,421,1088,1122,1352,1529,1639,1652), analgesics (288,485,604,727,1140,1636,1642,1648,1650) antihistamines (1635,1638,1639), sulfonamides (190,650,1227,1411,1472), antiseptics (102,194), isonicotinic acid hvdrazide (84, 86), phenazine dyes (145,1277), sulfonyl ureas (255), diuretics (771), zoalene (1453), ephedrine (675) and nikethamide derivatives (289). Pesticides.…”