Feedback phase correction of Bessel beams in confocal line light-sheet microscopy: a simulation study

Moosavi, S. Hoda; Gohn-Kreuz, Cristian

doi:10.1364/ao.52.005835

Cited by 14 publications

(14 citation statements)

References 16 publications

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“…14,15 It has also been suggested that dynamic elements could be used to control the illumination profile of a line scan illumination system for light sheet microscopy. 55 Micro-endoscopy Micro-endoscopes are attracting interest due to their potential in enabling optical biopsy for a range of clinical and research applications. The challenge in such systems is to perform high resolutionpossibly three-dimensional-imaging via a narrow endoscopes, whose dimensions constrain significantly the optical design.…”

Section: Light Sheet Microscopesmentioning

confidence: 99%

Adaptive optical microscopy: the ongoing quest for a perfect image

2014

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Adaptive optics is becoming a valuable tool for high resolution microscopy, providing correction for aberrations introduced by the refractive index structure of specimens. This is proving particularly promising for applications that require images from deep within biological tissue specimens. We review recent developments in adaptive microscopy, including methods and applications. A range of advances in different microscope modalities is covered and prospects for the future are discussed. INTRODUCTIONOptical microscopes have for a long time played a vital role in biomedical research. Their ability to provide multidimensional structural and functional information about a specimen in a non-invasive manner has permitted numerous scientific advances. These microscopes rely upon high-quality optical systems to operate at the optimum resolution, which is determined by the diffraction limit of light for conventional microscopes. However, even with perfect optical components, the performance of the microscope is affected by the optical properties of the specimen. Spatial variations in refractive index introduce aberrations as the light passes through the specimen-a problem that is exacerbated when imaging deeps into tissue. These aberrations detrimentally affect the resolution and contrast of microscope images, ultimately limiting the depth at which imaging is practical. Adaptive optics (AO) was introduced into microscopy in order to overcome this limitation. Dynamic correction elements, such as deformable mirrors (DMs) or spatial light modulators (SLMs) have been employed to compensate specimen-induced aberrations (Figure 1). New wavefront sensing and control schemes have been developed to adapt the correction elements.The first demonstrations of adaptive optical microscopy took place in the early 2000s. In the few years that followed, there were several innovative advances that set the scene for further research. The research in this period is well covered by two review articles 1,2 and a recent compiled volume, 3 so this will not be reproduced here. This present article provides a review of advances in aberration correction in adaptive microscopy that have taken place in the last half-decade. During this period, a wide range of AO techniques have emerged, which have considerably enhanced the available toolkit. Furthermore, the benefit of correction of specimen-induced aberrations has been shown in different areas, particularly (although not exclusively) in biomedical applications. We outline these technological

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Section: Light Sheet Microscopesmentioning

confidence: 99%

Adaptive optical microscopy: the ongoing quest for a perfect image

2014

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“…The use of Bessel‐beam illumination has recently been investigated for a variety of volumetric microscopy approaches . Specifically, for light sheet microscopy, the propagation‐invariant (“diffraction‐free”) property of the main lobe of a Bessel beam is valuable to generate a large depth of focus.…”

Section: Introductionmentioning

confidence: 99%

Bessel‐beam illumination in dual‐axis confocal microscopy mitigates resolution degradation caused by refractive heterogeneities

Chen

Glaser

Liu

2016

Journal of Biophotonics

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One of the main challenges for laser-scanning microscopy of biological tissues with refractive heterogeneities is the degradation in spatial resolution that occurs as a result of beam steering and distortion. This challenge is particularly significant for dual-axis confocal (DAC) microscopy, which achieves improved spatial-filtering and optical-sectioning performance over traditional confocal microscopy through off-axis illumination and collection of light with low-numerical aperture (NA) beams that must intersect precisely at their foci within tissues. DAC microscope image quality is sensitive to positional changes and distortions of these illumination- and collection-beam foci. Previous studies have shown that Bessel beams display improved positional stability and beam quality than Gaussian beams when propagating through tissues with refractive heterogeneities, which suggests that Bessel-beam illumination may enhance DAC microscopy of such tissues. Here, we utilize both Gaussian and Bessel illumination in a point-scanned DAC microscope and quantify the resultant degradation in resolution when imaging within heterogeneous optical phantoms and fresh tissues. Results indicate that DAC microscopy with Bessel illumination exhibits reduced resolution degradation from microscopic tissue heterogeneities compared to DAC microscopy with conventional Gaussian illumination.

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“…The "non-diffracting" property of a Bessel beam refers to its ability to propagate over a long distance along the optical axis without exhibiting any broadening or bending of its main lobe [1][2][3]. The "self-reconstructing" property refers to the ability of this main lobe to propagate through highly heterogeneous media, even in the presence of obstructions that may block or distort the main lobe temporarily [2,4,11]. This self-healing behavior is aided by the fact that each side lobe of a Bessel beam carries approximately the same amount of energy as the main lobe and continuously acts to reconstruct the main lobe as it propagates [12][13][14].…”

Section: Introductionmentioning

confidence: 99%

“…When performing light-sheet microscopy with conventional Gaussian illumination in tissues, the field of view (FOV), within which the thickness of the light sheet is fairly uniform, is restricted and may be approximated by the confocal parameter, or twice the Rayleigh range of the Gaussian beam [2,5,6]. In addition, tissue-heterogeneity-induced light-sheet distortions have been shown to diminish the contrast and resolution in Gaussian light-sheet-based microscopy [1,11]. The "non-diffracting" and "self-reconstructing" characteristics of a Bessel beam have shown promise for reducing the limitations mentioned above [1][2][3][4][5][6].…”

Section: Introductionmentioning

confidence: 99%

Characterizing the beam steering and distortion of Gaussian and Bessel beams focused in tissues with microscopic heterogeneities

Chen

Liu

2015

Biomed. Opt. Express

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Bessel beams have recently been investigated as a means of improving deep-tissue microscopy in highly scattering and heterogeneous media. It has been suggested that the long depth-of-field and selfreconstructing property of a Bessel beam enables an increased penetration depth of the focused beam in tissues compared to a conventional Gaussian beam. However, a study is needed to better quantify the magnitude of the beam steering as well as the distortion of focused Gaussian and Bessel beams in tissues with microscopic heterogeneities. Here, we have developed an imaging method and quantitative metrics to evaluate the motion and distortion of low-numerical-aperture (NA) Gaussian and Bessel beams focused in water, heterogeneous phantoms, and fresh mouse esophagus tissues. Our results indicate that low-NA Bessel beams exhibit reduced beam-steering artifacts and distortions compared to Gaussian beams, and are therefore potentially useful for microscopy applications in which pointing accuracy and beam quality are critical, such as dual-axis confocal (DAC) microscopy.

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Feedback phase correction of Bessel beams in confocal line light-sheet microscopy: a simulation study

Cited by 14 publications

References 16 publications

Adaptive optical microscopy: the ongoing quest for a perfect image

Adaptive optical microscopy: the ongoing quest for a perfect image

Bessel‐beam illumination in dual‐axis confocal microscopy mitigates resolution degradation caused by refractive heterogeneities

Characterizing the beam steering and distortion of Gaussian and Bessel beams focused in tissues with microscopic heterogeneities

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