Feeding Behavior ofDiaphorina citri(Hemiptera: Liviidae) and Its Acquisition of ‘CandidatusLiberibacter Asiaticus’, on Huanglongbing-InfectedCitrus reticulataLeaves of Several Maturity Stages

Luo, Xiao‐Zhu; Yen, Alan L.; Powell, Kevin; Wu, Fengnian; Wang, Yanjing; Zeng, Lixia; YuZhi, Yang; Cen, Yijing

doi:10.1653/024.098.0132

Cited by 46 publications

(46 citation statements)

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“…Insect feeding activities are related to pathogen acquisition and transmission to host plants (Carpane et al, 2011;Sandanayaka et al, 2014;Luo et al, 2015). Therefore, information regarding the transmission process and characteristics of pathogens is essential for developing effective disease control strategies.…”

Section: Discussionmentioning

confidence: 99%

“…Interactions between the stylet penetration activities of the sucking insects and the host plants play an important role in determining the disease transmission efficiency. The electrical penetration graph (EPG) technique has been used to study insect feeding behaviour linked to the transmission process of pathogens (Tjallingii, 1985;Jacobson & Kennedy, 2014;Luo et al, 2015;Wu et al, 2016). In our previous study, we described six EPG waveforms correlated with the stylet penetration activities during probing by the SCWL phytoplasma vector M. hiroglyphicus, of which the waveforms C and D were linked to acquisition and inoculation, respectively (Roddee et al, 2018).…”

Section: Introductionmentioning

confidence: 99%

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Characteristics of sugarcane white leaf phytoplasma transmission by the leafhopper Matsumuratettix hiroglyphicus

Roddee

Kobori

Hanboonsong

2018

Entomologia Exp Applicata

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The leafhopper Matsumuratettix hiroglyphicus (Matsumura) (Hemiptera: Cicadellidae) is the most important vector of sugarcane white leaf (SCWL) phytoplasma that significantly affects the sugarcane crop in Asia. Here, we aimed to study the characteristics of SCWL phytoplasma transmission by M. hiroglyphicus. To this end, the stylet penetration activities performed during the acquisition access period (AAP) and inoculation access period (IAP) were investigated by the direct current electrical penetration graph technique and confirmed by quantitative polymerase chain reaction (qPCR). Additionally, the latent period (LP) of SCWL phytoplasma in the vector was determined by qPCR and localised by fluorescent in situ hybridisation. The results indicated that the acquisition of SCWL phytoplasma occurred during phloem ingestion (waveform D), whereas its inoculation was associated with salivation into the phloem sieve element (waveform C). The minimum AAP was 15 min and the minimum duration of phloem ingestion was 2.35 min. The minimum LP of SCWL phytoplasma in the vector was at least 14 days; then, SCWL phytoplasma moved to the salivary glands of the insect, enabling the transmission of the pathogen to the host plants. The minimum IAP for a successful transmission of SCWL phytoplasma to the host plants was 11–12 min, with a minimum duration of salivation into phloem of 1.35 min. The female vectors had higher SCWL phytoplasma copy numbers than the male vectors, and displayed faster AAP, IAP, and LP. Overall, our findings provide important information related to the feeding behaviour of M. hiroglyphicus and its effect on the transmission of SCWL phytoplasma.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Characteristics of sugarcane white leaf phytoplasma transmission by the leafhopper Matsumuratettix hiroglyphicus

Roddee

Kobori

Hanboonsong

2018

Entomologia Exp Applicata

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“…Among these, 64% performed both E1 and E2 waveforms, and 29% performed only E1. In our previous study, we noticed two D. citri adults able to acquire Clas without showing E2 waveform (Luo et al, 2015). This was supported by the strong relationship between the average duration of phloem salivation (E1 waveform) and Clastransmission.…”

Section: Discussionmentioning

confidence: 62%

“…EPG recording methods were used as described by Luo et al (2015). The electrical penetration waveform signal is transformed to a digital signal by a DI_720 converter (DATAQ Instruments, Akron, OH, USA).…”

Section: Epg Monitoring and Clas Transmission Testmentioning

confidence: 99%

“…The typical waveforms C, G, E1, and E2 were identified and characterized for D. citri using EPG (Bonani et al, 2010;Yang et al, 2011;Serikawa et al, 2012). After characterization of the waveforms of D. citri, the EPG technique has been further used to study its feeding behavior in various situations such as in insecticide-treated plants (Bonani et al, 2010;Yang et al, 2011;Youn et al, 2011;Cen et al, 2012;Serikawa et al, 2012Serikawa et al, , 2013Luo et al, 2015). After characterization of the waveforms of D. citri, the EPG technique has been further used to study its feeding behavior in various situations such as in insecticide-treated plants (Bonani et al, 2010;Yang et al, 2011;Youn et al, 2011;Cen et al, 2012;Serikawa et al, 2012Serikawa et al, , 2013Luo et al, 2015).…”

Section: Introductionmentioning

confidence: 99%

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Feeding behavior of Diaphorina citri and its transmission of ‘Candidatus Liberibacter asiaticus’ to citrus

TianYu

Luo

et al. 2016

Entomologia Exp Applicata

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Huanglongbing (HLB), also known as citrus greening, is currently the most destructive disease of citrus, responsible for huge economic losses in the world's major citrus production areas. The Asian citrus psyllid, Diaphorina citri Kuwayama (Hemiptera: Liviidae), transmits 'Candidatus Liberibacter asiaticus' (Clas), the pathogen responsible to cause HLB. Understanding of vector, pathogen, and host plant interactions is important for the management of this vector-disease complex. We used the direct-current electrical penetration graph (DC-EPG) system to evaluate feeding behavior of Clasinfected D. citri adults, and their potential to transmit the pathogen to healthy citrus, Citrus reticulata Blanco cv. Sunki (Rutaceae), following a 24-h inoculation access period. Plants were tested for the presence of Clas by qPCR 6 months after inoculation. Findings suggest that inoculation was associated with salivation into the phloem sieve elements (waveform E1). The minimum feeding time for successful transmission by a single adult was 88.8 min, with a minimum E1 duration of 5.1 min. Regression analysis indicated a significant relationship between E1 duration and transmission efficiency. The adults successful in transmitting Clas to healthy citrus were able to penetrate and feed in the phloem much earlier than those which did not transmit. The minimum duration of E1 for a female was shorter than that of a male, but transmission was higher. However, durations of other EPG parameters were not significantly different between male and female. Feeding by single Clasinfected D. citri adults on 6-month-old plants (Sunki) resulted in 23% HLB-positive plants 6 months after inoculation. Multiple nymphs or adults could transmit the pathogen more efficiently than individual adults in the field, and further enhance the severity of the disease. Effective tactics are warranted to control D. citri and disrupt transmission of Clas.

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Plant jasmonic acid mediated contrasting effects of two citrus aphid species onDiaphorina citriKuwayama

Gao

Tao

Arthurs³

et al. 2022

Pest Management Science

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Background Herbivores may influence each other directly and through plant mediated inter‐specific interactions. The Asian citrus psyllid (Diaphorina citri Kuwayama) and citrus aphids are key pests that can co‐exist on citrus, but their plant‐mediated interaction between them is unknown. Here we investigated plant‐mediated effect of two citrus aphid species, the polyphagous Aphis spiraecola Patch and the oligophagous Aphis (Toxoptera) citricidus (Kirkaldy) on the feeding behavior and reproduction of Diaphorina citri, and explored the underlying mechanisms. Results In comparison with those on aphid free plants, Diaphorina citri had decreased reproduction and reduced phloem feeding on Aphis spiraecola pre‐infested plants, while the reproduction and feeding efficiency were increased on Aphis citricidus pre‐infested plants. Jasmonic acid (JA) dependent defense was significantly activated by Diaphorina citri feeding on Aphis spiraecola pre‐infested plants, but was suppressed by Diaphorina citri feeding on Aphis citricidus pre‐infested plants compared with that on aphid free plant. By contrast, only one tested marker gene in salicylic acid (SA) signaling was activated by Diaphorina citri feeding on Aphis spiraecola pre‐infested plants. Furthermore, exogenous application of methyl jasmonate, but not SA, conferred resistance against Diaphorina citri in our citrus trials. Conclusion Our results indicate that pre‐infestation by two citrus aphid species differentially altered Diaphorina citri induced citrus JA dependent defense, which resulted in different effect on subsequent Diaphorina citri performance. © 2022 Society of Chemical Industry.

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Feeding Behavior ofDiaphorina citri(Hemiptera: Liviidae) and Its Acquisition of ‘CandidatusLiberibacter Asiaticus’, on Huanglongbing-InfectedCitrus reticulataLeaves of Several Maturity Stages

Abstract: BioOne Complete (complete.BioOne.org) is a full-text database of 200 subscribed and open-access titles in the biological, ecological, and environmental sciences published by nonprofit societies, associations, museums, institutions, and presses.

Cited by 46 publications

References 32 publications

Characteristics of sugarcane white leaf phytoplasma transmission by the leafhopper Matsumuratettix hiroglyphicus

Characteristics of sugarcane white leaf phytoplasma transmission by the leafhopper Matsumuratettix hiroglyphicus

Feeding behavior of Diaphorina citri and its transmission of ‘Candidatus Liberibacter asiaticus’ to citrus

Plant jasmonic acid mediated contrasting effects of two citrus aphid species onDiaphorina citriKuwayama

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