Feeding Bugs to Bugs: Edible Insects Modify the Human Gut Microbiome in an in vitro Fermentation Model

Young, Wayne; Arojju, Sai Krishna; McNeill, M.R.; Rettedal, Elizabeth A.; Gathercole, Jessica; Bell, N.L.; Payne, Penny R.

doi:10.3389/fmicb.2020.01763

Cited by 19 publications

(15 citation statements)

References 47 publications

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“…In addition to this, the genus Prevotella(phylum Bacteroidetes) was most abundant in one species of spider i.e.Cyclosa mulmeinensis which may be due to digested grass grub larvae. This report is also observed in human gut microbiome which was modified due to digestion of grass grub larvae (Young et al 2020).…”

Section: Discussionsupporting

confidence: 61%

Inter subfamily comparison of gut microbial diversity in twelve wild spider species of family Araneidae

Tyagi¹,

Tyagi²,

Prasad³

et al. 2020

Preprint

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Spiders are among the most diverse groups of arthropods remarkably known for extra oral digestion. The largest effort based on targeted 16S amplicon next generation sequencing was carried out to decipher the inter subfamily comparison of gut bacterial diversity in spiders and their functional relationship. Twelve spider species belonging to three subfamilies, Araneinae (8), Argiopinae (2) and Gasteracanthinae (2) of family Araneidae have been studied. Analysis revealed the presence of 22 phyla, 145 families, and 364 genera of microbes in the gut microbiome, with Proteobacteria as the highest abundant Phylum. Moreover, the phyla Firmicutes, Actinobacteria and Deinococcus Thermus were also detected. The bacterial phyla Bacteriodetes and Chlamydiae dominated in Cyclosa mulmeinensis and Neoscona bengalensis respectively. At genera level, Acinetobacter, Pseudomonas, Cutibacterium, Staphylococcus, and Bacillus were the most dominant genera in their gut. In addition to this, the genus Prevotella was observed only in one species, Cyclosa mulmeinensis, and endosymbiont genus Wolbachia generally responsible for reproductive alterations was observed in one spider species Eriovixia laglaizei. Our study revealed that the gut bacterial diversity of the spiders collected from wild are quite different from the diet driven spider gut bacterial diversity as published earlier. A functional analysis revealed the involvement of gut microbiota in carbohydrate, lipid, amino acids, fatty acids and energy metabolism.

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Section: Discussionsupporting

confidence: 61%

Inter subfamily comparison of gut microbial diversity in twelve wild spider species of family Araneidae

Tyagi¹,

Tyagi²,

Prasad³

et al. 2020

Preprint

View full text Add to dashboard Cite

show abstract

“…Prepare samples for the following analyses; metagenomics, metabolomics, metatranscriptomics, organic acids, headspace gases, sulfide, dry matter, and organic matter. Extract DNA from the fermentation pellet and fecal aliquots and conduct whole-genome sequencing Use the NucleoSpin Soil kit following the manufacturer’s instructions with the addition of a bead-beating step for 4 min to extract DNA from the samples (BioSpec Mini-Beadbeater 96, Bartlesville, OK, United States) ( Young et al., 2020 ) Prepare libraries using the Illumina TruSeq DNA PCR-free kit Sequence DNA on an Illumina MiSeq with 24–30 million paired-end reads per run (read length 2 × 150 base pairs), and output of 3–4.5 gigabytes per run Analyze one set of supernatant samples by Liquid Chromatography-Mass Spectrometry (LC-MS) and Gas Chromatography-Mass Spectrometry (GC-MS) to determine the relative abundance of all detectable metabolites. For LC-MS ( Vanden Bussche et al., 2015 ): Thaw supernatant samples and centrifuge at 13,300 × g for 5 min Syringe filter sample through a 13 mm diameter, 0.22 μm pore size Polyvinylidene Fluoride filter Dilute sample 1:5 with ultrapure water Run sample through the LC-MS with an HSS-T3 C18 column (1.8 μm, 150 mm × 2.1 mm) Note: This is a reversed-phase column which will not retain highly polar molecules, allowing semi-polar and non-polar molecules to elute for MS analysis.…”

Section: Step-by-step Methods Detailsmentioning

confidence: 99%

“…Extract DNA from the fermentation pellet and fecal aliquots and conduct whole-genome sequencing Use the NucleoSpin Soil kit following the manufacturer’s instructions with the addition of a bead-beating step for 4 min to extract DNA from the samples (BioSpec Mini-Beadbeater 96, Bartlesville, OK, United States) ( Young et al., 2020 ) Prepare libraries using the Illumina TruSeq DNA PCR-free kit Sequence DNA on an Illumina MiSeq with 24–30 million paired-end reads per run (read length 2 × 150 base pairs), and output of 3–4.5 gigabytes per run …”

Section: Step-by-step Methods Detailsmentioning

confidence: 99%

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A protocol combining breath testing and ex vivo fermentations to study the human gut microbiome

et al. 2021

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Summary This protocol describes the application of breath testing and ex vivo fermentations to study the association between breath methane and the composition and functionality of the gut microbiome. The protocol provides a useful systems biology approach for studying the gut microbiome in humans, which combines standardized methods in human breath testing and fecal sampling. The model described is accessible and easy to repeat, but its relative simplicity means that it can deviate from human physiological conditions.

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“…Additives such as: -Dietary emulsifiers: sodium CMC, P80, soy lecithin, sophorolipids, and rhamnolipids. [34,50,[54][55][56][57][58][59] To assay the gut microbiota metabolism in response to food components.…”

Section: Main Objective Study Subject Referencesmentioning

confidence: 99%

In Vitro Simulation of Human Colonic Fermentation: A Practical Approach towards Models’ Design and Analytical Tools

et al. 2021

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The human colonic microbiota plays an important role in the food digestion process and has a key role in maintaining health status. This community of microbes is inter-individually different due to several factors that modulate its composition. Among them, diet is one of the most relevant, which, in turn, is affected by environmental, economic, and cultural considerations. These pieces of evidence have promoted the study of the influence of diet on gut microbiota and the development of in vitro models that simulate the colonic digestion of foods. This narrative review aims to present a technical approach of the in vitro gut models available to evaluate the impact of diet on human colonic microbiota. A description and comments on the main characteristics, parameters, applicability, faecal inoculum preparation, and analytical tools are made. Despite the progress of in vitro colonic digestion models and metaomic applicability in this research field, there are still some challenges to face due to the lack of a consensus on the methodologies to conduct in vitro colonic digestions and the need to integrate the metaomic data to fully understand the influence of food in human colonic microbiota.

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Feeding Bugs to Bugs: Edible Insects Modify the Human Gut Microbiome in an in vitro Fermentation Model

Cited by 19 publications

References 47 publications

Inter subfamily comparison of gut microbial diversity in twelve wild spider species of family Araneidae

Inter subfamily comparison of gut microbial diversity in twelve wild spider species of family Araneidae

A protocol combining breath testing and ex vivo fermentations to study the human gut microbiome

In Vitro Simulation of Human Colonic Fermentation: A Practical Approach towards Models’ Design and Analytical Tools

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