Feeding-dependent depression of melanin-concentrating hormone and melanin-concentrating hormone receptor-1 expression in vagal afferent neurones

Cocaine- and Amphetamine-Regulated Transcript: Stimulation of Expression in Rat Vagal Afferent Neurons by Cholecystokinin and Suppression by Ghrelin

“…However, the regulation of CART expression by CCK in vagal afferent neurons adds an additional dimension to our understanding of this system. Although other examples of CCK-regulated expression of signaling molecules associated with feeding behavior have been described previously (Burdyga et al, 2004(Burdyga et al, , 2006b, in each case CCK was found to depress expression of genes associated with orexigenic function (e.g., CB1 and MCH-1 receptors and MCH). The present findings suggest that activation of CCK-1 receptors on vagal afferent neurons is also associated with increased expression of a gene associated with satiety signaling.…”

“…Previous studies have shown that expression of the orexigenic peptide MCH in vagal afferent neurons is increased by fasting and decreased by CCK (Burdyga et al, 2006b). We therefore asked whether the loss of CART in serum-starved vagal afferent neurons was associated with increased MCH (Fig.…”

“…In addition, a growing body of evidence now indicates regulation by CCK of gene expression in these neurons. Thus, energy restriction of rats for 12-18 h or longer sharply increases expression of CB1 and MCH-1 receptors, and of MCH, and refeeding downregulates expression via CCK (Burdyga et al, 2004(Burdyga et al, , 2006b). This is not a general response of all receptors associated with orexigenic function because OX-R1 and GHS-1 expression appears unchanged in these circumstances (Burdyga et al, 2003(Burdyga et al, , 2006a.…”

“…Recent studies suggest that in addition to expression of CCK-1 receptors, vagal afferent neurons also express the leptin receptor (Ob-R) and receptors associated with stimulation of food intake including the ghrelin (GHS-1), cannabinoid (CB1), orexin (OX-R1), and MCH-1 receptors (Kirkup et al, 2001;Burdyga et al, 2002Burdyga et al, , 2003Burdyga et al, , 2004Burdyga et al, , 2006b. Orexin A and ghrelin inhibit the discharge of vagal afferent neurons in response to CCK compatible with integration of both orexigenic and anorexigenic stimuli outside the CNS (Burdyga et al, 2003;Date et al, 2005).…”

Anti-melanin-concentrating hormone treatment attenuates chronic experimental colitis and fibrosis

“…Studies in the rat revealed numerous MCH-containing cells, some of them enterochromaffin cells, in gastric antrum and corpus, duodenum, and colon (19). Our own group has demonstrated MCH and MCHR1 immunoreactivity in the rat myenteric and submucosal plexus (24), whereas others have shown that vagal afferent neurons in both human and rat express MCH and its G protein-coupled seven-transmembrane receptor (MCHR1) (6). Moreover, when tested in an in situ ligated loop experiment, MCH stimulated Na ϩ and K ϩ fluxes in proximal colon; water, as well as Na ϩ and K ϩ fluxes in duodenum; and bicarbonate absorption in the jejunum (20).…”