FEEDING EFFECT OF INULIN DERIVED FROM Dahlia variabilis TUBER ON INTESTINAL MICROBES IN STARTER PERIOD OF CROSSBRED NATIVE CHICKENS

Abstract

Penelitian ini bertujuan untuk mengkaji efek pemberian inulin dari umbi Dahlia variabilis, baik dalam bentuk tepung maupun ekstrak, terhadap keberadaan mikroba usus halus pada ayam kampung persilangan periode starter. Materi penelitian adalah 280 ekor ayam kampung persilangan unsex, dan tepung maupun ekstrak umbi dahlia sebagai sumber inulin yang mulai diberikan pada umur 22 hari. Penelitian menggunakan rancangan acak lengkap dengan 7 perlakuan dan 4 ulangan (masing-masing 10 ekor ayam). Perlakuan terdiri dari : T0 (ransum basal/RB), T1 (RB+0,4% tepung umbi dahlia), T2 (RB +0,8% tepung umbi dahlia), T3 (RB+1,2% tepung umbi dahlia), T4 (RB+0,39% ekstrak umbi dahlia), T5 (RB+0,78% ekstrak umbi dahlia), dan T6 (RB+1,17% ekstrak umbi dahlia). Parameter yang diamati adalah bakteri asam laktat (BAL), Escherichia coli, pH digesta usus halus (duodenum, jejunum dan ileum), laju digesta, dan pertambahan bobot badan harian (PBBH). Data dianalisis ragam dan dilanjutkan dengan uji wilayah ganda Duncan, serta polinomial ortogonal. Hasil penelitian menunjukkan bahwa pemberian inulin dalam bentuk tepung maupun ekstrak umbi dahlia nyata (P<0,05) meningkatkan populasi BAL dan PBBH, tetapi menurunkan jumlah Escherichia coli, pH, dan memperlambat laju digesta. Simpulan penelitian adalah pemberian inulin dari umbi dahlia dalam bentuk tepung optimum pada taraf 0,9% dan dalam bentuk ekstrak masih linier sampai taraf 1,17%. Kata kunci : ayam kampung persilangan, inulin umbi dahlia, mikroba usus halus, pertambahan bobot badan harian ABSTRACT The purpose of the research was to study the effects of feeding inulin derived from Dahlia variabilis tuber powder and extract on the existence of intestinal microbes in crossbred native chicken starter. Experimental animals were 280 unsex crossbred native chickens and powder and extract of dahlia tuber as source of inulin, which were started to be fed on day 22. The present experiment was assigned in a completely randomized design with 7 treatments and 4 replications (10 birds each). The treatments were: T0 (basal diet/BD), T1 (BD+0.4% powder form), T2 (BD+0.8% powder form), T3 (BD+1.2% powder form), T4 (BD+0.39% extract form), T5 (BD+0.78% extract form), and T6 (BD+1.17% extract form). Parameters measured were the number of Lactic acid bacteria (LAB), Escherichia coli, intestinal potential hydrogen (pH) (duodenal, jejunal and ileal), rate of passage and daily body weight gain (DBWG). Data were subjected to ANOVA and followed by Duncan and polynomial orthogonal test. The results indicated that feeding inulin derived from dahlia tuber powder and extract form significantly (P<0.05) increased LAB population and DBWG, but decreased Escherichia coli number and intestinal pH, and slow the rate of passage. In conclusion, feeding inulin of dahlia tuber powder was optimum at 0.9% but that of extract is linier until 1.17%. Keywords : crossbred native chicken, inulin of dahlia tuber, intestinal microbes, daily gain Inulin Derived from Dahlia variabilis Tuber on Intestinal Microbes (L. Krismiyanto et al.

FEEDING EFFECT OF INULIN DERIVED FROM DAHLIA TUBER COMBINED WITH Lactobacillus sp. ON MEAT PROTEIN MASS OF CROSSBRED KAMPONG CHICKEN

“…The decrease in pathogenic bacteria population was an indication of the improved health status of the gastrointestinal tract that bring about the increase in crossbred kampong chicken body resistance. This evidence supported by previous results (Krismiyanto et al, 2014) that E.coli count reduced with feeding inulin in starter period of crossbred native chicken. The improvement of health status of gastrointestinal tract implicated to the improved antioxidant activity in body tissue.…”

Meat Characteristic of Crossbred Local Chicken Fed Inulin of Dahlia Tuber and Lactobacillus sp.

“…This result is in agreement with the report of Krismiyanto et al (2014) that the increased lactic acid bacteria (LAB) population was due to the feeding effect of inulin derived from dahlia tuber which can intensely fermented by endogenous beneficial bacteria. Thus, the drecreased fat meat content and cholesterol concentration found in the present study can be associated with the increase in bile salt hydrolase (BSH) enzyme produced by the enhanced growth of LAB (Fajrih et al, 2014).…”

Enterococcus faecium NCIMB 10415 supplementation improves the meat quality and antioxidant capacity of muscle of broilers

“…Peran et al (2007) and Rajput et al (2013) have also reported that probiotics can prevent oxidative damage to the small intestine caused by pathogenic bacteria by releasing GSH-Px enzyme as an antioxidant. We speculate that the increased antioxidant activity in body tissues of broilers by E. faecium supplementation might be associated with the improvement of the health of the gastrointestinal tract, which has been proposed by a previous study (Krismiyanto, Suthama, & Wahyuni, 2014).…”