Feeding microparticle protein sources composed-diet with addition of natural additive to produce clean product of broiler for consumer health friendly

Abstract

Evaluation of feeding diet composed of microparticle protein source added with inulin of chicory root and Lactobacillus acidophilus on intestinal bacteria population, protein digestibility, and performance of broiler was the purpose of the present study. Experimental animals were 248 birds of day old broiler with body weight of 48.2±0.50g (240 birds for the main experiment, and 8 birds for endogenous correction). Feed composed of microparticle protein source added with inulin of chicory and Lactobacillus acidophilus at 1.2%, respectively. A completely randomized design with five treatments and six replications (8 birds each) was set in the present study. Treatments tested were R0: diet with 20% intact protein (control); R1: diet with 18% microparticle protein, R2: R1 + 1.2% inulin of chicory; R3: R1 + 1.2% Lactobacillus acidophilus; and R4: R1 + 1.2% Lactobacillus acidophilus + 1.2% inulin of chicory. Total lactic acid bacteria (LAB) and coliform, protein digestibility, and broiler performances (muscle protein mass, feed intake (FI), body weight gain (BWG), and feed conversion (FCR) were the parameters observed. Data were subjected to analysis of variance and continued to Duncan test at 5% probability level. LAB population significantly (P<0.05) increased, and in contrast, coliform count and FCR significantly (P<0.05) decreased in R2, R3 and R4. Protein digestibility and BWG improved in R3 and R4, but FI enhanced only in R4. In conclusion, feed composed of microparticle protein sources with addition of either chicory inulin or Lactobacillus acidophilus at 1.2%, respectively, increase total LAB, protein digestibility and growth performances of broilers. The combination of both additives have more profound effect on productivity and produce a healthy meat product indicated by high muscle protein mass derived from host animal with low pathogen.

Antioxidant Enzymes and Growth of Broiler Fed Microparticle Protein Diet with Inulin or Lactobacillus acidophillus Supplementation

“…The determination of meat protein and fat mass were performed based on a formula as previously reported (Suthama, 2003). Productive performances, including body weight gain (BWG), feed consumption, and feed conversion ratio (FCR), were measured according to the most recently reported (Suthama et al, 2021).…”

“…The report of Hosseinifard et al (2020) provided clarification that rats given inulin in combination with probiotic Lactobacillus plantarum or the inclusion of probiotic bacteria alone demonstrated the reduced oxidative MDA level and increased SOD concentration as the oxidative enzyme marker. The high availability of protein and minerals, especially Ca, due to the use of feed composed of microparticle protein source (Suthama et al, 2021) would be greatly possible supported by prebiotic inulin or L. acidophilus supplementation that acts as a positive impact on oxidative enzyme function. However, more scientific description is needed to clarify the mechanism clearly through the advanced study concerning the relationship between inulin or Lactobacillus sp.…”

“…Microparticle protein can be basic information in establishing the use of processed protein source ingredients for poultry that gives impact in maximizing nutrient utilization and minimizing gut diseases due to the pathogenic bacteria, and finally, improved growth (Suthama et al, 2021b). Lactic acid bacteria (LAB) population and villus height increased, and growth performance improved in broiler given graviola (Annona muricata Linn.)…”