Feeding pure docosahexaenoate or arachidonate decreases plasma triacylglycerol secretion in rats

Williams, M. A.; Tinoco, J.; Y, Yang; Bird, Michael I.; Hincenbergs, I.

doi:10.1007/bf02544579

Cited by 29 publications

(6 citation statements)

References 54 publications

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“…Therefore, it might be that the number of double bonds in the structure is an important factor for the enhancement of intercellular degradation of Apo B100. The serum TG level of the DHA-TG group was not significantly different from that of the control group, which is not consistent with other reports that DHA decreases blood TG levels , . A possible reason for this discrepancy is that the blood TG lowering effects of DHA might be due to a reduction of the hepatic TG level.…”

Section: Resultscontrasting

confidence: 93%

Effects of Three Different Highly Purified n-3 Series Highly Unsaturated Fatty Acids on Lipid Metabolism in C57BL/KsJ-db/db Mice

Gotoh

Nagao

Onoda

et al. 2009

J. Agric. Food Chem.

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Triglycerides (TG) consisting of highly purified (>97%) n-3 series highly unsaturated fatty acids, eicosapentaenoic acid (EPA), docosapentaenoic acid (DPA), and docosahexaenoic acid (DHA), were administered to C57BL/KsJ-db/db mice for 4 weeks by pair-feeding to compare their effects on lipid metabolism and to evaluate the effects of DPA on lipid metabolism. The hepatic TG level and total amount was decreased by treatment with DHA and DPA compared to the control. The efficacy of DPA was greater than that of EPA, but less than that of DHA. In contrast, EPA had the greatest serum TG reducing effect. The hepatic cytosol fraction of the DHA-treated group contained the lowest fatty acid synthase (FAS) and malic enzyme (ME) activity levels. Furthermore, the DHA-treated group contained the highest serum adiponectin concentrations. These findings indicate that the strong hepatic TG-lowering effect of DHA is due to the suppression of TG synthesis. The same tendencies were observed in DPA-treated mice, and the effect was stronger than that observed in EPA-treated mice, but equivalent to that observed in DHA-treated mice. Based on these results, DPA possesses lipid metabolism-improving effects. The beneficial effects of DPA for lipid metabolism were not superior to those of EPA and DHA, and the effect was always intermediate between those of EPA and DHA.

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Section: Resultscontrasting

confidence: 93%

Effects of Three Different Highly Purified n-3 Series Highly Unsaturated Fatty Acids on Lipid Metabolism in C57BL/KsJ-db/db Mice

Gotoh

Nagao

Onoda

et al. 2009

J. Agric. Food Chem.

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“…Finally, the Sar-1/COPII complex is necessary when apo B-100 exits the hepatic ER [256] and apo B-48 containing chylomicrons are exported from the enterocyte [248]. However, the Sar-1/COPII complex is also implicated in the exiting of CFTR from the ER [257] for its entry into the proteasome degradation pathway [258]. Overall, these observations demonstrate the existence of possible interactions among apo B elaboration, chylomicron packaging and CFTR function.…”

Section: Enterocyte Phase Abnormalitiesmentioning

confidence: 99%

Mechanisms of lipid malabsorption in Cystic Fibrosis: the impact of essential fatty acids deficiency

et al. 2005

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Transport mechanisms, whereby alimentary lipids are digested and packaged into small emulsion particles that enter intestinal cells to be translocated to the plasma in the form of chylomicrons, are impaired in cystic fibrosis. The purpose of this paper is to focus on defects that are related to intraluminal and intracellular events in this life-limiting genetic disorder. Specific evidence is presented to highlight the relationship between fat malabsorption and essential fatty acid deficiency commonly found in patients with cystic fibrosis that are often related to the genotype. Given the interdependency of pulmonary disease, pancreatic insufficiency and nutritional status, greater attention should be paid to the optimal correction of fat malabsorption and essential fatty acid deficiency in order to improve the quality of life and extend the life span of patients with cystic fibrosis.

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“…Fukazawa et al (12) reported decreased triglyceride (TG) and phospholipid (PL) secretion from perfused livers of EFAdeficient (EFAD) rats. However, EFA deficiency has also been associated with enhanced hepatic TG secretion rates in rats (6,19,49,50). Similarly, data on lipoprotein clearance during EFA deficiency are equivocal.…”

mentioning

confidence: 99%

Essential fatty acid deficiency in mice is associated with hepatic steatosis and secretion of large VLDL particles

Werner¹,

Havinga²,

Bos³

et al. 2005

American Journal of Physiology-Gastrointestinal and Liver Physi

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. Essential fatty acid deficiency in mice is associated with hepatic steatosis and secretion of large VLDL particles. Am J Physiol Gastrointest Liver Physiol 288: G1150 -G1158, 2005. First published January 20, 2005 doi:10.1152/ajpgi.00456.2004.-Essential fatty acid (EFA) deficiency in mice decreases plasma triglyceride (TG) concentrations and increases hepatic TG content. We evaluated in vivo and in vitro whether decreased hepatic secretion of TG-rich very low-density lipoprotein (VLDL) contributes to this consequence of EFA deficiency. EFA deficiency was induced in mice by feeding an EFAdeficient (EFAD) diet for 8 wk. Hepatic VLDL secretion was quantified in fasted EFAD and EFA-sufficient (EFAS) mice using the Triton WR-1339 method. In cultured hepatocytes from EFAD and EFAS mice, VLDL secretion into medium was measured by quantifying [3 H]-labeled glycerol incorporation into TG and phospholipids. Hepatic expression of genes involved in VLDL synthesis and clearance was measured, as were plasma activities of lipolytic enzymes. TG secretion rates were quantitatively similar in EFAD and EFAS mice in vivo and in primary hepatocytes from EFAD and EFAS mice in vitro. However, EFA deficiency increased the size of secreted VLDL particles, as determined by calculation of particle diameter, particle sizing by light scattering, and evaluation of the TG-to-apoB ratio. EFA deficiency did not inhibit hepatic lipase and lipoprotein lipase activities in plasma, but increased hepatic mRNA levels of apoAV and apoCII, both involved in control of lipolytic degradation of TG-rich lipoproteins. EFA deficiency does not affect hepatic TG secretion rate in mice, but increases the size of secreted VLDL particles. Present data suggest that hypotriglyceridemia during EFA deficiency is related to enhanced clearance of altered VLDL particles. hepatic lipoprotein secretion; lipoprotein clearance; hypotriglyceridemia DEVELOPMENT OF HEPATIC STEATOSIS is a well-established manifestation of essential fatty acid (EFA) deficiency in animal models. It was first described in 1958 in rats by Alfin-Slater and Bernick (3) and in 1970 by Fukazawa et al. (12) and Sinclair and Collins (43). The excess lipid deposited in the liver during EFA deficiency can theoretically result from increased uptake of circulating lipids, enhanced de novo lipogenesis, decreased fatty acid oxidation, or decreased hepatic lipoprotein secretion, or from a combination of these. Increased hepatic lipogenesis and decreased fatty acid oxidation could indeed contribute, because polyunsaturated fatty acids are physiological suppressors of fatty acid synthesis (4, 31) through downregulation of SREBP1c (16, 42, 51) and inducers of hepatic fatty acid oxidation through activation of PPAR␣, respectively. The quantitative contribution of increased lipogenesis and decreased oxidation to EFA deficiency-induced hepatic steatosis has not been established. Whereas the effects of EFA deficiency on induction of hepatic steatosis are fairly consistent in the literature, the consequences for he...

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Feeding pure docosahexaenoate or arachidonate decreases plasma triacylglycerol secretion in rats

Cited by 29 publications

References 54 publications

Effects of Three Different Highly Purified n-3 Series Highly Unsaturated Fatty Acids on Lipid Metabolism in C57BL/KsJ-db/db Mice

Effects of Three Different Highly Purified n-3 Series Highly Unsaturated Fatty Acids on Lipid Metabolism in C57BL/KsJ-db/db Mice

Mechanisms of lipid malabsorption in Cystic Fibrosis: the impact of essential fatty acids deficiency

Essential fatty acid deficiency in mice is associated with hepatic steatosis and secretion of large VLDL particles

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