Feeding strategies to optimize vanillin production by Amycolatopsis sp. ATCC 39116

Valério, Rita; Bernardino, Ana R S; Torres, Cristiana A.V.; Brazinha, Carla; Tavares, Maria L.; Crespo, João G.; Reis, Maria A.M.

doi:10.1007/s00449-020-02482-7

Cited by 25 publications

(21 citation statements)

References 36 publications

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“…Production of bio‐vanillin (hereafter referred to as vanillin) through the microbial conversion of natural precursors (such as ferulic acid (FA), eugenol, isoeugenol, and vanillic acid) using bacteria, fungi, yeast, or genetically engineered microorganisms has been extensively reported 3‐8 . Among these precursors, FA is the most explored due to its natural abundance and structural similarity to vanillin 2 .…”

Section: Introductionmentioning

confidence: 99%

“…Among these precursors, FA is the most explored due to its natural abundance and structural similarity to vanillin 2 . Several studies related to the bioproduction of vanillin from FA showed that Actinomycetes , particularly of the genera Amycolatopsis , is one of the most promising microorganisms because it can produce a relatively high yield of vanillin while exhibiting a high tolerance toward FA and vanillin 2,8 …”

Section: Introductionmentioning

confidence: 99%

“…1,2 Production of bio-vanillin (hereafter referred to as vanillin) through the microbial conversion of natural precursors (such as ferulic acid (FA), eugenol, isoeugenol, and vanillic acid) using bacteria, fungi, yeast, or genetically engineered microorganisms has been extensively reported. [3][4][5][6][7][8] Among these precursors, FA is the most explored due to its natural abundance and structural similarity to vanillin. 2 Several studies related to the bioproduction of vanillin from FA showed that Actinomycetes, particularly of the genera Amycolatopsis, is one of the most promising microorganisms because it can produce a relatively high yield of vanillin while exhibiting a high tolerance toward FA and vanillin.…”

Section: Introductionmentioning

confidence: 99%

“…2 Several studies related to the bioproduction of vanillin from FA showed that Actinomycetes, particularly of the genera Amycolatopsis, is one of the most promising microorganisms because it can produce a relatively high yield of vanillin while exhibiting a high tolerance toward FA and vanillin. 2,8 Ferulic acid is a phenolic compound that can be obtained from agro-waste, such as sugar beet pulp, 9 maize bran, 9 sugarcane bagasse, 10 corn cobs, 11 wheat bran, [12][13] flax shives, 12 corn bran, 12 and rye bran. 13 In herbaceous plants, such as corn (Zea mays L.), phenolic compounds (including p-coumaric acid (p-CA) and ferulic acid) are covalently bound to lignin and hemicellulose via ester and ether bonds, forming lignin/phenolics-carbohydrate complexes (LCC).…”

Section: Introductionmentioning

confidence: 99%

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Enhancing the bioconversion of ferulic acid from alkaline hydrolysate of corn cobs to vanillin by Amycolatopsis thermoflava under nutrient limitation and reducing sugar control

Nirwana

Hung

Shu

2022

J of Chemical Tech & Biotech

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BACKGROUND The use of commercially available ferulic acid (FA) as a precursor for vanillin production is costly. The utilization of agro‐waste as a starting material provides an alternative to obtain FA at a low cost and establish an economically profitable production system. In this work, optimization of ultrasound‐assisted extraction conditions for releasing FA from corn cobs using alkaline treatment was conducted. Further, bioconversion of FA from the crude hydrolysate of corn cobs to vanillin using Amycolatopsis thermoflava was performed. RESULTS Since autoclaved hydrolysate was unfavorable for cell growth and vanillin formation, pasteurization was proposed. However, the presence of contaminants limited the entire process. Aiming to suppress the growth of contaminants and increase vanillin yield, novel strategies of limiting and controlling the nutrients for the strain were developed. Optimal hydrolysis conditions (NaOH concentration = 0.5 M, solid loading = 10% for 30 min) produced hydrolysate with relatively high titers of FA (715 mg L−1) and p‐coumaric acid (1025 mg L−1). To improve vanillin production from FA, four parameters (including the effects of sterilization method, nutrient limitation, initial biomass concentration, and reducing sugar control) were investigated. By adopting these strategies and performing bioconversion of FA to vanillin with an initial biomass concentration of 1.5 g L−1, the yield of vanillin was significantly increased by 29.5‐fold to 477.4 mg g−1, compared to bioconversion using autoclaved hydrolysate. CONCLUSION This approach represents a relatively economical method for vanillin production from agro‐waste because it uses crude hydrolysate, requires minimum nutrients, and involves non‐modified organisms with a relatively short bioconversion time (17 h). © 2022 Society of Chemical Industry (SCI).

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Enhancing the bioconversion of ferulic acid from alkaline hydrolysate of corn cobs to vanillin by Amycolatopsis thermoflava under nutrient limitation and reducing sugar control

Nirwana

Hung

Shu

2022

J of Chemical Tech & Biotech

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“…Table 6 illustrates the relative fraction of the protonated form of vanillin (HVan), ferulic acid (H 2 Fer), vanillic acid (H 2 Vac), alcohol vanillyl (Halc) and guaiacol (HGuai) at different pH values, including at pH 8.2 (the pH value at the end of the vanillin bioconversion [51]) and at pH 7.2 (the pH value reported in [19] as optimal for vanillin bioconversion). Table 6 shows that at pH 8.2, only 13.7% of vanillin is in protonated form, which has a negative effect on permeation if a pervaporation process is used [26]; the ferulic acid and vanillic acid that remain in the bioconversion broth are in their non-volatile, non-protonated (ionic) form.…”

Section: Optimisation Of Feed Ph Used In the Pervaporation And Vacuum...mentioning

confidence: 99%

Comparative Analysis of Bio-Vanillin Recovery from Bioconversion Media Using Pervaporation and Vacuum Distillation

2022

Self Cite

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The increasing demand for natural products has led to biotechnological vanillin production, which requires the recovery of vanillin (and vanillyl alcohol at trace concentrations, as in botanical vanillin) from the bioconversion broth, free from potential contaminants: the substrate and metabolites of bioconversion. This work discusses the recovery and fractionation of bio-vanillin, from a bioconversion broth, by pervaporation and by vacuum distillation, coupled with fractionated condensation. The objective was to recover vanillin free of potential contaminants, with maximised fluxes and selectivity for vanillin against water and minimised energy consumption per mass of vanillin recovered. In vacuum distillation fractionated condensation, adding several consecutive water pulses to the feed increased the percentage of recovered vanillin. In pervaporation-fractionated condensation and vacuum distillation-fractionated condensation processes, it was possible to recover vanillin and traces of vanillyl alcohol without the presence of potential contaminants. Vacuum distillation–experiments presented higher vanillin fluxes than pervaporation fractionated condensation experiments, 2.7 ± 0.1 g·m−2 h−1 and 1.19 ± 0.01 g·m−2 h−1, respectively. However, pervaporation fractionated condensation assures a selectivity of vanillin against water of 4.5 on the pervaporation step (acting as a preconcentration step) and vacuum distillation fractionated condensation requires a higher energy consumption per mass of vanillin recovered when compared with pervaporation– fractionated condensation, 2727 KWh kgVAN−1 at 85 °C and 1361 KWh kgVAN−1 at 75 °C, respectively.

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Combining genetic engineering and bioprocess concepts for improved phenylpropanoid production

Virklund¹,

Jensen

Nielsen

et al. 2022

Biotech & Bioengineering

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The group of natural aromatic compounds known as phenylpropanoids has diverse applications, but current methods of production which are largely based on synthesis from petrochemicals or extraction from agricultural biomass are unsustainable. Bioprocessing is a promising alternative, but improvements in production titers and rates are required to make this method profitable. Here the recent advances in genetic engineering and bioprocess concepts for the production of phenylpropanoids are presented for the purpose of identifying successful strategies, including adaptive laboratory evolution, enzyme engineering, in‐situ product removal, and biocatalysis. The pros and cons of bacterial and yeast hosts for phenylpropanoid production are discussed, also in the context of different phenylpropanoid targets and bioprocess concepts. Finally, some broad recommendations are made regarding targets for continued improvement and areas requiring specific attention from researchers to further improve production titers and rates.

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Feeding strategies to optimize vanillin production by Amycolatopsis sp. ATCC 39116

Cited by 25 publications

References 36 publications

Enhancing the bioconversion of ferulic acid from alkaline hydrolysate of corn cobs to vanillin by Amycolatopsis thermoflava under nutrient limitation and reducing sugar control

Enhancing the bioconversion of ferulic acid from alkaline hydrolysate of corn cobs to vanillin by Amycolatopsis thermoflava under nutrient limitation and reducing sugar control

Comparative Analysis of Bio-Vanillin Recovery from Bioconversion Media Using Pervaporation and Vacuum Distillation

Combining genetic engineering and bioprocess concepts for improved phenylpropanoid production

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