Feline Embryo Transfer during the Non-Breeding Season.

Tsutsui, Toshihiko; I, Yamane; Hattori, Ikuko; Kurosawa, Noriko; Matsunaga, Hideo; Murao, Ikuko; Kanda, Masayuki; Hori, Tatsuya

doi:10.1292/jvms.62.1169

Cited by 18 publications

(20 citation statements)

References 10 publications

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“…According to Wildt et al (1978), although being efficient in stimulating ovarian activity, FSH presents a short half-life, requiring various daily applications that can cause hyperstimulation. The use of eCG, at doses of 50 and 150 IU, had extremely satisfactory results with a single dose (Pope et al, 1998;Donoghue et al, 1992;Goodrowe et al, 1988;Tsutsui et al, 2000 ). In our study, the use of 150 IU eCG was effective in inducing follicular development in all animals tested.…”

Section: Discussionsupporting

confidence: 54%

“…Several protocols have been published in the literature for induction of ovarian activity, including the use of equine chorionic gonadotropin (eCG), pregnant mare serum gonadotropin (PMSG) or hypofisary follicle stimulating hormone (FSH-p), Platz, et al 1978;Dresser et al, 1988;Howard et al, 1992;Pope et al, 1993;Kanda et al, 1995;Pope et al, 1998;Tsutsui et al, 2000;Mattos et al ., 2003). According to Wildt et al (1978), although being efficient in stimulating ovarian activity, FSH presents a short half-life, requiring various daily applications that can cause hyperstimulation.…”

Section: Discussionmentioning

confidence: 99%

“…In our study, the use of 150 IU eCG was effective in inducing follicular development in all animals tested. The use of human chorionic gonadotropin has been reported in all studies on induction of ovulation in cats previously stimulated for follicular development, although in a wide range of doses Platz et al 1978;Dresser et al, 1988;Howard et al, 1992;Pope et al, 1993;Kanda et al, 1995;Pope et al, 1998;Tsutsui et al, 2000;Mattos et al 2003). In this experiment, the dose of 100 IU hCG, 4 h after pre-stimulation with eCG, was effective in promoting ovulation in all the animals used.…”

Section: Discussionmentioning

confidence: 99%

“…Dominion over the repeatability and adaptation of assisted reproduction protocols in the domestic cat is very valuable for development and adaptation for wild species, and it may play an important role in the conservation of endangered species in the near future. Thus, the domestic cat has been used intensively as an experimental model for different techniques, including: in vitro embryo maturation and fertilization (Bowen, 1977;Byers et al, 1992;Roth et al, 1994;Karja et al, 2002), embryogenesis and embryo migration , in vitro embryo development (Goodrowe et al 1988;Kanda et al, 1995;Pope et al, 1998;Skrzyszowska et al, 2002;Gómez et al, 2003), artificial ovulation induction (Greulich, 1934;Tsutsui et al, 1989), culture (Jewgenow et al, 1995;, transfer (Platz et al, 1978;Goodrowe et al, 1988;Wood et al, 1995;Pope, 2000;Tsutsui et al, 2000;Kitiyanant et al, 2003) and embryo cryopreservation (Ballou, 1992;Leibo & Songsasen, 2002).…”

Section: Introductionmentioning

confidence: 99%

“…Recovery in the domestic cat should be made between 6 and 9 days after mating (Kraemer et al,1979). Swanson & Godke (1994) proposed a less invasive transcervical technique for embryo recovery and transfer, but the most-used technique is surgery (Kraemer et al, 1979;Dresser et al, 1988;Kanda et al, 1995;Pope, 2000;Tsutsui et al, 2000).…”

Section: Introductionmentioning

confidence: 99%

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Exogenous induction of ovarian activity and ovulation and transfer of fresh embryos of domestic cat (Felis catus)

2012

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Indução exógena da atividade ovariana e da ovulação e transferência de embriões a fresco em gatas domésticas (Felis catus)Os objetivos deste trabalho foram avaliar a estimulação exógena da atividade do ovário e a definição de protocolos para recolhimento e transferência de embriões em gatos domésticos, para aplicação potencial em espécies selvagens ameaçadas de extinção. Foram utilizados 16 gatas domésticas adultas e dois machos adultos reprodutores, mantidos no gatil experimental do setor de Morfologia do DVT-UFV. Todas as fêmeas receberam uma única aplicação de 150 UI de Gonadotrofina Coriônica Equina (eCG) no pós-estro, como indutor da atividade ovariana e, 80 a 84 horas após, receberam uma única aplicação de 100 UI de Gonadotrofina Coriônica Humana (hCG), como indutor da ovulação. Após a aplicação de hCG, apenas as gatas doadoras foram naturalmente acasaladas. As gatas receptoras receberam estímulo extra de indução da ovulação por meio da manipulação de um swab intravaginal. Cinco a seis dias após a aplicação de hCG, as gatas doadoras foram submetidas a uma laparotomia, para a coleta dos embriões, a qual foi efetuada mediante The objective of the present study was the exogenous stimulation of ovarian activity and definition of embryo collection, and transfer protocols, in the domestic cat for potential application in non-domestic endangered species. Sixteen adult queens and two adult male reproducers kept in the experimental cat house at the Morphology sector at the Veterinary Department (DVT), UFV, were used in this study. All the queens received a single application of 150 IU Equine Chorionic Gonadotropin (eCG) in the post estrus to induce ovarian activity and 80 to 84 hours later, received a single application of 100 UI Human Chorionic Gonadotropin (hCG) to induce ovulation. After hCG application, only the donor queens were naturally mated. The receptor queens received extra stimulus for induction of ovulation through manipulation of an intravaginal swab. Five to six days after hCG application, the donor queens were subjected to a laparotomy for embryo collection that was performed by trans-horn uterine washing. On average, six embryos were surgically inovulated. They were classified as type I and III compact morula and blastocysts in four receptor queens. Three animals presented pregnancy confirmed by ultrasound at day 36 and two of these animals gave birth to litters of two and four offsprings, respectively, at 66 and 63 days after induction of ovulation. Except for one still birth, all the offspring developed normally.

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