Feline Giardiasis in Turkey: Prevalence and Genetic and Haplotype Diversity of Giardia duodenalis Based on the β-Giardin Gene Sequence in Symptomatic Cats

Sürsal, Neslihan; Şimşek, Emrah; Yıldız, Kader

doi:10.1645/19-183

Cited by 15 publications

(11 citation statements)

References 55 publications

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“…Therefore, in the present study, cats with pasty stools (44%) were significantly ( p < 0.05) more likely to be infected than those that had firm stools (16%) ( Table 2 ). These findings agree with those of other studies in which G. duodenalis was detected more frequently from animals with diarrhea [ 53 , 54 , 55 ].…”

Section: Discussionsupporting

confidence: 93%

Occurrence of Giardia duodenalis in Cats from Queretaro and the Risk to Public Health

Veyna-Salazar

Cantó-Alarcón

Olvera-Ramírez

et al. 2023

Animals

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Giardia is a protozoan that affects humans as well as a wide range of domestic species. It is distributed worldwide, and the highest frequency is seen in developing countries. Due to the potential for domestic cats to be carriers of this parasite and subsequently transmit the infection to humans, it is important to know the risk of transmission. For this reason, the objective of this study was to determine the frequency of this parasite in the cat population of the city of Santiago de Queretaro, Mexico, and identify the assemblages present to determine the role this host plays in public health, this being the first study of its type to be performed in the country. This was a cross-sectional study during which 200 fecal samples were collected from cats of both sexes and varying ages and strata of origin. The samples were analyzed by microscopy following the flotation technique, having obtained a general frequency of 25%. Giardia cysts were found at higher frequency in pasty stools. The assemblages found were zoonotic, specifically assemblage A, which suggests that the cat poses an important risk for the dissemination of the parasite to humans, making it an important public health problem.

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Section: Discussionsupporting

confidence: 93%

Occurrence of Giardia duodenalis in Cats from Queretaro and the Risk to Public Health

Veyna-Salazar

Cantó-Alarcón

Olvera-Ramírez

et al. 2023

Animals

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“…When dealing with G. duodenalis genotyping, small subunit ribosomal RNA (SSU‐rDNA) and β‐giardin gene ( bg ) are ordinarily used, respectively, at assemblage and sub‐assemblage levels. In particular, the bg region seems to be the most efficient to reveal isolates diversity from feline faecal samples (Sursal et al., 2020 ).…”

Section: Introductionmentioning

confidence: 99%

Giardia duodenalis in colony stray cats from Italy

Procesi

Carnio

Berrilli

et al. 2021

Zoonoses and Public Health

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Giardia duodenalis is the most common intestinal protozoan in humans and animals worldwide, including eight morphologically identical assemblages, infecting pets, livestock, wildlife and human beings. Assemblages A and B are those with the higher zoonotic potential, and they have been detected in several mammals other than humans; the others (C to H) show a higher host specificity. Cats can harbour both the specific Assemblage F and the zoonotic ones A and B. Several studies have been carried out on G. duodenalis genotypes in cats; however, the role of this species in the epidemiology of giardiasis is still poorly understood. In this scenario, the present study carried out the detection and genetic characterization at sub-assemblage level of G. duodenalis from colony stray cats in central Italy. In the period 2018-2019, 133 cat faecal samples were analysed for the presence of G. duodenalis cysts by a direct immunofluorescence assay. Positive samples were subsequently subjected to molecular analyses for assemblage/sub-assemblage identification. Forty-seven samples (35.3%) were positive for G. duodenalis cysts by immunofluorescence. G. duodenalis DNA was amplified at SSU-rDNA locus from 39 isolates: 37 were positive for zoonotic Assemblage A and 2 showed a mixed infection (A + B). Positive results for the β-giardin gene were achieved for 25 isolates. Sequence analysis revealed 16 isolates belonging to Sub-assemblage AII and 8 to Sub-assemblage AIII. One isolate resulted as ambiguous AI/AIII. Large sequence variability at the sub-assemblage level was detected, with several double peaks and mutations, making complex a proper isolate allocation. When compared with previous studies, the 35.3% prevalence of G. duodenalis in cats reported in the present article was surprisingly high. Moreover, all positive cats resulted to be infected with zoonotic assemblages/sub-assemblages, thus indicating stray cats as a possible source of human giardiasis and highlighting the sanitary relevance of cat colonies in the study area.

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“…In this study, the prevalence of Giardia duodenalis in lambs detected 23.33% and 29.17% by microscobic and Nested-PCR method, respectively. These values are lower than the results of some researchers (3,4,22), are similar to some (17), and are higher than some researchers' studies (16,20,21). Geographical locations, different climates, sample sizes, animal species, age of the animal, and methods employed in research can be counted among the reasons for the differences observed between studies.…”

Section: Discussionmentioning

confidence: 51%

“…In other studies, 4.1% prevalence in calves and 1.4% in cattle in Sivas was reported (20), while 18.8% prevalence in dogs was reported in Aydın (21). 68.6% prevalence in cats in the Central Anatolia Region (22), 64.7% in calves, and 36.3% in goats in Van (18) were also reported. A prevalence of 20% was reported in samples taken from water sources in Isparta (23).…”

Section: Discussionmentioning

confidence: 73%

A Microscopic and Molecular Survey of Giardia duodenalis in Lambs in Siirt, Türkiye

ÇELİK,

ASLAN ÇELİK,

AYAN

et al. 2023

Dicle Üniversitesi Veteriner Fakültesi Dergisi

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Giardia duodenalis is a parasite that causes gastrointestinal diseases and is widespread worldwide. This parasite, which causes infections especially in lambs, is highly prevalent in farm animals. In this study, the prevalence and risk factors of G. duodenalis in lambs were investigated. Lamb fecal samples were collected during the study and microscopic and molecular examinations were performed using Nested PCR method. While Giardia sp. cysts were detected in 28 (23.33%) of 120 samples, the DNA of the parasite was detected in 35 (29.17%) of 120 samples by molecular examination. At the end of the study, the prevalence of G. duodenalis in lambs was recorded between 23.33% and 29.17%. The highest prevalence rate among age groups was found in 16-30 days old lambs, while the highest positivity rate among locations was found in Şirvan district and the lowest positivity rate was found in the center. According to gender, the disease prevalence was higher in females than in males. However, there was no statistically significant difference between location, gender and age groups (p>0.05). In conclusion, G. duodenalis infestations are commonly found in lambs in Siirt province. The results of this study emphasize that control measures and hygiene practices should be implemented to prevent and control G. duodenalis infestations in lambs.

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Feline Giardiasis in Turkey: Prevalence and Genetic and Haplotype Diversity of Giardia duodenalis Based on the β-Giardin Gene Sequence in Symptomatic Cats

Cited by 15 publications

References 55 publications

Occurrence of Giardia duodenalis in Cats from Queretaro and the Risk to Public Health

Occurrence of Giardia duodenalis in Cats from Queretaro and the Risk to Public Health

Giardia duodenalis in colony stray cats from Italy

A Microscopic and Molecular Survey of Giardia duodenalis in Lambs in Siirt, Türkiye

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