Femoral µCT Analysis, Mechanical Testing and Immunolocalization of Bone Proteins in β-Hydroxy β-Methylbutyrate (HMB) Supplemented Spiny Mouse in a Model of Pregnancy and Lactation-Associated Osteoporosis

Abstract

A metabolite of leucine, ß-hydroxy-ß-methylbutyrate (HMB), used as a dietary supplement effects muscle tissue gain and bone tissue quality. Since there are no studies on the effects of HMB during pregnancy yet, the aim of the current study was to determine the effects of HMB supplementation during pregnancy on osteoporotic bone quality postpartum and post-lactation using spiny mice (Acomys cahirinus) as the animal models. The six-month-old dams were divided into four groups: pregnant and lactating controls, and pregnant and lactating HMB-treated (during the second trimester of pregnancy) females. The intensity of the immunoreaction of osteocalcin (OC), osteoprotegerin (OPG), bone morphogenetic protein 2 (BMP-2), tissue inhibitor of metalloproteinases 2 (TIMP-2), matrix metalloproteinase 8 and 13 (MMP-8 and MMP-13) and proteins involved in bone turnover, was measured in femoral trabecular and compact bone, as well as in the hyaline and epiphyseal cartilage of the femora. The analysis of the trabecular bone microarchitecture showed that the administration of HMB to pregnant females, by influencing the proteins responsible for bone cell activity and collagen remodeling, can provide protection from bone loss. Based on the results of the current study it can be assumed that HMB administration to pregnant females has a more positive impact on trabecular than compact bone.

Bioinformatics analysis of key genes, immune infiltration, and risk assessment in low bone mineral density among perimenopausal women: An observational study

“…These findings suggest a possible correlation between MMP8 levels and decreased bone density in premenopausal women. [15] The regulation of HMOX1 expression is susceptible to hormonal fluctuations, particularly the changes in estrogen levels observed during perimenopause. Estrogen is recognized for its ability to modulate HMOX1 expression, and a decrease in estrogen levels may have implications for HMOX1 levels, potentially influencing the skeletal health of perimenopausal women.…”

Influence of mechanical stimuli and zoledronic acid on the femoral morfology in rats with obesity and limited mobility models

“…Згідно зі статистичними даними, частим місцем остеопоротичних переломів є шийка стегнової кістки та поперековий відділ хребта. Проте в літературі є недостатньо досліджень, які б характеризували стан дистальної частини стегнової кістки, де швидкість метаболізму є вищою, що підтверджено імунною реакцією клітин [8,9]. У дослідженні нашою метою було порівняти вплив загальної вібрації (ЗВ) та золедронової кислоти (Зол.)…”