Femtomolar DNA detection by parallel colorimetric darkfield microscopy of functionalized gold nanoparticles

Verdoold, R.; Gill, Ron; Ungureanu, F.; Molenaar, Robert; Kooyman, R.P.H.

doi:10.1016/j.bios.2011.06.019

Cited by 36 publications

(25 citation statements)

References 33 publications

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“…Briefly, the Au nanoparticles were randomly immobilized by drop‐coating 1 μL of the diluted Au nanoparticle solution (OD 520 ≈ 0.04) onto the center of a freshly coated APTES slide for 2 min at room temperature before being rinsed off with water. This resulted in a coverage of Au nanoparticles such that individual nanoparticles had sufficient interparticle distance to be individually visible under the dark‐field microscope 36. Au nanoparticle concentration and incubation time were used to control interparticle spacing.…”

Section: Methodsmentioning

confidence: 99%

Amplification of Resonant Rayleigh Light Scattering Response Using Immunogold Colloids for Detection of Lysozyme

Truong¹,

Choi²,

Sim³

2013

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A strategy for attomolar-level detection of small molecule-size proteins is reported based on Rayleigh light scattering spectroscopy of individual nanoplasmonic aptasensors by exploiting the outstanding characteristics of gold colloids to amplify the nontransparent resonant signal at ultralow analyte concentrations. The fabrication method utilizes thiol-mediated adsorption of a DNA aptamer on the immobilized Au nanoparticle surface, the interfacial binding characteristics of the aptamer with its target molecules, and the antibody-antigen interaction through plasmonic resonance coupling of the Au nanoparticles. Using lysozyme as a model analyte for disease detection, the detection limit of the aptasensor is ∼7 × 10(3) aM, corresponding to the LSPR λmax shift of ∼2.25 nm. Up to a 380% increase in the localized resonant λmax shift is demonstrated upon antibody binding to the analyte compared to the primary response during signal amplification using immunogold colloids. This enhancement leads to a limit of detection of ∼7 aM, which is an improvement of three orders of magnitude. The results demonstrate substantial promise for developing coupled plasmonic nanostructures for ultrasensitive detection of various biological and chemical analytes.

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Section: Methodsmentioning

confidence: 99%

Amplification of Resonant Rayleigh Light Scattering Response Using Immunogold Colloids for Detection of Lysozyme

Truong¹,

Choi²,

Sim³

2013

Small

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show abstract

“…[ 40 ] For example, secondary AuNCs can bind to the AuNC arrays of the 3rd‐tier via biomolecules, resulting in a color change or spectral shift for biodetection with high sensitivity. [ 57 ] Further increasing the annealing duration to 20 h at 1000 °C induced a significant decrease in the size of the AuNCs (diameter 51 ± 5 nm). Meanwhile, AuNCs started to be engulfed into the SiO 2 NPs (

{Au}_{15s}^{200W} T_{20h}^{1000 ° C}

@SPCs, the close‐up top‐view HR‐SEM image in Figure 3d), accompanied by the formation of silica ridges (indicated by the red arrows in Figure 3d,e).…”

Section: Resultsmentioning

confidence: 99%

Multilevel Spherical Photonic Crystals with Controllable Structures and Structure‐Enhanced Functionalities

Wang

Le‐The

Shui

et al. 2020

Advanced Optical Materials

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Spherical photonic crystals (SPCs) with tailorable multiscale structure, versatile surface morphology, and controllable optical properties of both photonic stop band (PSB) and surface plasmon resonance (SPR), have been fabricated using a robust and facile method. The fabricated SPCs consist of well‐spaced gold nanocrystals (AuNCs) (3rd‐tier) anchored on silica nanopatterns (2nd‐tier) confined in microspherical templates (1st‐tier). Droplet microfluidics is used to produce microdroplets containing silica nanoparticles (SiO2NPs) which assemble to form two‐tier SPCs. Subsequently, three‐tier SPCs are obtained by thermal dewetting and evaporation of metal films deposited on the two‐tier SPCs, with the 3rd‐tier morphology being controlled by the deposited film morphology and programmed thermal annealing. Optical PSB and SPR properties of the prepared SPCs can be on‐demand tailored by the 2nd and 3rd‐tier morphology and their corresponding constituent materials. It is found that the scattering from AuNC arrays on the SPCs can be amplified by tailoring the PSB properties. The hierarchical SPCs manufactured by this method take advantages of low‐cost, high controllability, and further processability. The manufacturing of flexible film encapsulated well‐assembled SPCs as anticounterfeiting stamps, which are easy to be identified using the mobile phone with a flash, is demonstrated.

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“…5.13 [42]. In addition, to enhance the sensitivity of the coupling effect between nanoparticles, a hairpin-loop DNA was used to increase the length extension after the DNA was hybridised [43,44].…”

Section: Interparticle Coupling-enhanced Biosensorsmentioning

confidence: 99%

Interparticle Coupling-Enhanced Detection

Long

Jing

2014

SpringerBriefs in Molecular Science

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When the distances between two or more plasmonic nanoparticles are very small, the plasmon resonance scattering spectra are greatly enhanced and distinct colour changes occur due to the coupling of the particles. Similar to fluorescence resonance energy transfer, plasmonic coupling is also distance dependent. Thus, researchers have fabricated colorimetric sensors by modulating the distance between nanoparticles, which have been used in a wide variety of applications, including DNA hybridisation, heavy-metal-ion detection, and protein binding. In this chapter, we primarily focus on the coupling of single particles, which enables the single-molecule detection through enhanced sensitivity. Keywords Fundamentals of Plasmonic CouplingThe coupling of plasmonics enhances the resonance intensity significantly and enables variable sensitive biosensors [1][2][3][4][5][6][7][8][9][10][11]. The factors that affect the coupling of nanoparticles include their particle size, coupling number, distance, direction, and shape [12][13][14][15][16][17]. Lee calculated the influence of particle size, number, composition, and distance using the generalised multiparticle Mie formalism [18]. The satellites in this work were made of gold with a relative permeability μ = 1. Cores composed of either gold or glass were considered. The surrounding medium was assumed to be free space with a permittivity ε = μ = 1. The permittivity of the glass core was 2.25. As shown in Fig. 5.1a, as the satellite number increased, the scattering peak wavelength exhibited a nearly linear redshift. For r core = 50 nm, r sat = 10 nm, and d sat = 2 nm, the redshift was approximately 1 nm per satellite. Although these calculations may not accurately reflect absolute quantities, they reveal the trends in the peak wavelength as the number of satellites increases.

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Femtomolar DNA detection by parallel colorimetric darkfield microscopy of functionalized gold nanoparticles

Cited by 36 publications

References 33 publications

Amplification of Resonant Rayleigh Light Scattering Response Using Immunogold Colloids for Detection of Lysozyme

Amplification of Resonant Rayleigh Light Scattering Response Using Immunogold Colloids for Detection of Lysozyme

Multilevel Spherical Photonic Crystals with Controllable Structures and Structure‐Enhanced Functionalities

Interparticle Coupling-Enhanced Detection

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