Femtomole detection of amino acids and dipeptides by gas chromatography—negative-ion chemical ionization mass spectrometry following alkylation with pentafluorobenzyl bromide

Márquez, Cristina D.; Weintraub, Susan T.; Smith, Philip C.

doi:10.1016/0378-4347(94)00247-9

Cited by 26 publications

(15 citation statements)

References 15 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The characterization of amino acids is vital for assessing protein quality, predicting nutritional status, and also for the improvement of culture conditions for the production of amino acids in food industry [1][2][3]. The availability of rapid, sensitive and reliable methods for amino acid analysis is highly desirable.Traditionally, the analytical methodologies applied to amino acid analysis include gas chromatography (GC) [4,5], capillary electrophoresis (CE) [6] and high performance liquid chromatography (HPLC) [6] coupled with either spectroscopy or mass spectrometry (MS) detectors. Laborious sample preparation or derivatization and long assay times are the major disadvantages of these methods.…”

mentioning

confidence: 99%

“…Traditionally, the analytical methodologies applied to amino acid analysis include gas chromatography (GC) [4,5], capillary electrophoresis (CE) [6] and high performance liquid chromatography (HPLC) [6] coupled with either spectroscopy or mass spectrometry (MS) detectors. Laborious sample preparation or derivatization and long assay times are the major disadvantages of these methods.…”

mentioning

confidence: 99%

See 1 more Smart Citation

Fragmentation pathway for glutamine identification: Loss of 73 da from dimethylformamidine glutamine isobutyl ester

Zhang

Wysocki

Scaraffia

et al. 2005

J. Am. Soc. Mass Spectrom.

View full text Add to dashboard Cite

A fragmentation mechanism for the neutral loss of 73 Da from dimethylformamidine glutamine isobutyl ester is investigated. Understanding this mechanism will allow to improve the identification and quantification of 15N-labeled and unlabeled glutamine and the distinguishing of glutamine and glutamic acid by electrospray ionization (ESI)-tandem mass spectrometry. Before mass spectrometry analysis, glutamine and glutamic acid are derivatized with dimethylformamide dimethyl acetal and isobutanol to form dimethylformamidine isobutyl ester. Derivatization conditions are modified based on an existing method to ensure complete derivatization of glutamic acid and to prevent the hydrolysis of glutamine. The fragmentation mechanism of dimethylformamidine glutamine isobutyl ester is studied and possible fragmentation pathways are proposed. Based on the fragmentation mechanism, a quantification method is developed to quantify both 15N-labeled and unlabeled glutamine and glutamic acid at a series of different neutral losses by performing multiple-reaction monitoring (MRM) scans in a triple-quadrupole mass spectrometer. Labeled glutamine includes 15N-amide labeled, 15N-amine labeled glutamine and glutamine 15N-labeled at both amide and amine positions. Deuterium labeled glutamine and glutamic acid are used as internal standards. Isotope effects are characterized for 15N labeled and deuterium labeled glutamine. It is found that the same method can be used to distinguish aspartic acid from asparagine. This study will improve the application of MS/MS for amino acid quantification and stable isotope labeling metabolism studies.

show abstract

mentioning

confidence: 99%

mentioning

confidence: 99%

Fragmentation pathway for glutamine identification: Loss of 73 da from dimethylformamidine glutamine isobutyl ester

Zhang

Wysocki

Scaraffia

et al. 2005

J. Am. Soc. Mass Spectrom.

View full text Add to dashboard Cite

show abstract

“…In the early days, dedicated isotope ratio mass spectrometers with a very high precision were applied to collect the required data [4], while more recently the less expensive combination of gas chromatography and mass spectrometry (GC-MS) has been popularized [5,6]. Today however, atmospheric pressure ionization (API) techniques like electrospray enable the coupling of liquid chromatography with mass spectrometry (LC-MS).…”

Section: Introductionmentioning

confidence: 99%

Measurement of amino acid isotope enrichment by liquid chromatography mass spectroscopy after derivatization with 9-fluorenylmethylchloroformate

Eijk

Suylen

Dejong

et al. 2007

Journal of Chromatography B

View full text Add to dashboard Cite

“…All these sample preparation steps are necessary as well for GC-MS [7][8][9][10][11], LC-MS [12], LC-MS/MS [13] and HPLC analyses [14,15]. The analysis of amino acids is time consuming due to sample preparation and analysis time.…”

Section: Introductionmentioning

confidence: 99%

Application of liquid chromatography-tandem mass spectrometry (LC–MS/MS) for the analysis of stable isotope enrichments of phenylalanine and tyrosine

Meesters

Wolfe

Deutz

2009

Journal of Chromatography B

View full text Add to dashboard Cite

Femtomole detection of amino acids and dipeptides by gas chromatography—negative-ion chemical ionization mass spectrometry following alkylation with pentafluorobenzyl bromide

Cited by 26 publications

References 15 publications

Fragmentation pathway for glutamine identification: Loss of 73 da from dimethylformamidine glutamine isobutyl ester

Fragmentation pathway for glutamine identification: Loss of 73 da from dimethylformamidine glutamine isobutyl ester

Measurement of amino acid isotope enrichment by liquid chromatography mass spectroscopy after derivatization with 9-fluorenylmethylchloroformate

Application of liquid chromatography-tandem mass spectrometry (LC–MS/MS) for the analysis of stable isotope enrichments of phenylalanine and tyrosine

Contact Info

Product

Resources

About