Femtosecond absorption spectroscopy of cytochrome c oxidase: Excited electronic states and relaxation processes in heme a and heme a3 centers

Individual heme a and heme a3 contributions to the Soret absorption spectrum of the reduced bovine cytochrome c oxidase

“…photobleaching spectra obtained by excitation with pump pulses centered at 570, 608 and 623 nm exc . The 608exc spectrum qualitatively resembles the CcO photobleaching spectrum reported recently for a similar pump-probe protocol with the excitation at 600 nm [35,36]. The exc spectra differ in amplitude since the photoexcitation yield depends on the absorption strength in the region of excitation and on the pump energy.…”