Femtosecond laser-induced cleaving of protein crystal in water solution

Kashii, Masafumi; Hosokawa, Yoichiroh; Kitano, Hiroshi; Adachi, Hiroaki; Mori, Yusuke; Takano, Kazufumi; Matsumura, Hideki; Inoue, Tsuyoshi; Murakami, Satoshi; Sugamoto, Kazuomi; Yoshikawa, Hideki; Sasaki, T.; Masuhara, Hiroshi

doi:10.1016/j.apsusc.2007.01.106

Cited by 9 publications

(9 citation statements)

References 12 publications

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“…Focusing the laser beam with an objective lens further enables one to modify, stimulate and manipulate biological samples with high spatial precision. For example, NIR fs laser has been utilized to cut protein crystals in aqueous solution [ 7 ], to cleave chromosomes [ 8 ], to ablate axons in live C. elegan s [ 9 ], to disrupt organelles [ 10 , 11 ] and cells [ 8 , 12 ], and to dissect tissues [ 13 ]. Besides, it has been applied to fabricate adhesive substrates to pattern cells under a culturing condition, and to control cell migration and cell-cell interactions [ [14] , [15] , [16] ].…”

Section: Introductionmentioning

confidence: 99%

Selective induction of targeted cell death and elimination by near-infrared femtosecond laser ablation

Okano

Wang

Hong

et al. 2020

Biochemistry and Biophysics Reports

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The techniques for inducing the death of specific cells in tissue has attracted attention as new methodologies for studying cell function and tissue regeneration. In this study, we show that a sequential process of targeted cell death and removal can be triggered by short-term exposure of near-infrared femtosecond laser pulses. Kinetic analysis of the intracellular accumulation of trypan blue and the assay of caspase activity revealed that femtosecond laser pulses induced immediate disturbance of plasma membrane integrity followed by apoptosis-like cell death. Yet, adjacent cells showed no sign of membrane damage and no increased caspase activity. The laser-exposed cells eventually detached from the substrate after a delay of >54 min while adjacent cells remained intact. On the base of in vitro experiments, we applied the same approach to ablate targeted single cardiac cells of a live zebrafish heart. The ability of inducing targeted cell death with femtosecond laser pulses should find broad applications that benefit from precise cellular manipulation at the level of single cells in vivo and in vitro .

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Section: Introductionmentioning

confidence: 99%

Selective induction of targeted cell death and elimination by near-infrared femtosecond laser ablation

Okano

Wang

Hong

et al. 2020

Biochemistry and Biophysics Reports

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“…The experiments, where crystals of the final product already exist in the solution, have been excluded from our definition. According to this definition, the literature − has considerably increased in the last five years (Figure S1). On the one hand, the observation of laser-induced nucleation is repeatable for various types of compounds, and on the other hand it seems possible to control crystallization by manipulating laser parameters.…”

Section: Introductionmentioning

confidence: 99%

Non-Photochemical Laser-Induced Nucleation of Sulfathiazole in a Water/Ethanol Mixture

Ikni

Scouflaire

et al. 2016

Crystal Growth & Design

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Non-photochemical laser-induced nucleation (NPLIN) is an original and promising nucleation method that has been applied with success to small-molecule organic compounds, proteins, and inorganic compounds, leading to almost 50 publications. After the first application of NPLIN to glycine and carbamazepine, our team has performed NPLIN experiments on sulfathiazole (STZ) for the following reasons: (i) STZ is considered as a model drug for studying polymorphism; (ii) some STZ polymorphs present a two-dimensional packing type, while others have one-dimensional packing; (iii) STZ solubility curves, crystal habits, and crystallization characterization are well documented. First, we have determined the metastable zone limit of STZ in water/ethanol (v/v 1:1) for three temperatures (15, 25, and 40 °C). STZ metastable solutions have been irradiated with a nonfocused nanosecond laser (532 nm) at 25 °C. Nucleation induction time and crystal habits have been compared with those obtained by spontaneous nucleation. Nucleation site is the air/solution interface. We have noted Ind 50 (β) the laser intensity required to induce nucleation with 50% efficiency for a given supersaturation coefficient β. The use of this index has allowed us to compare NPLIN experiments for different compounds in different solutions. A dependency of STZ crystal size and crystal number on the irradiation duration, i.e., pulse number, has been established. Moreover, the obtained crystals have been characterized by Raman spectroscopy and X-ray single crystal diffraction. The role of laser polarization (linear or circular) has been established in comparison with spontaneous nucleation. In order to gain a deeper understanding of this behavior, we have calculated ab initio interaction energies for all dimers existing in the different STZ polymorphs. Theoretical results are coherent with observations.

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“…15,18,32, 33 The results of MD simulations of laser ablation of molecular targets performed in the regime of stress confinement, 17,29,30 however, suggest that the contribution of thermoelastic stresses can also significantly affect the ablation yield and parameters of the ejected plume at laser fluences above the threshold for the onset of explosive boiling. More numerous clusters with higher ejection velocities are produced by phase explosion in the regime of stress confinement as compared to simulations performed at the same laser fluences but with longer pulses, away from the stress confinement conditions.…”

Section: Introductionmentioning

confidence: 99%

“…The ejection of large liquid droplets or solid particulates caused by the relaxation of the laser-induced stresses is often called photomechanical ablation or spallation. [15][16][17]32,33 The maximum values of the laserinduced stresses and contribution of photomechanical effects to the material ejection or damage are defined by the relationship between the characteristic time of the laser heating and the time required for the mechanical relaxation (expansion) of the heated volume, τ s . When the time of the laser heating is shorter than τ s , the heating and melting take place under a nearly constant volume condition, causing a build up of compressive stresses.…”

Section: Introductionmentioning

confidence: 99%

Atomistic Modeling of Short Pulse Laser Ablation of Metals: Connections between Melting, Spallation, and Phase Explosion

2009

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The mechanisms of short pulse laser interactions with a metal target are investigated in simulations performed with a model combining the molecular dynamics method with a continuum description of laser excitation, electron-phonon equilibration, and electron heat conduction. Three regimes of material response to laser irradiation are identified in simulations performed with a 1 ps laser pulse, which corresponds to the condition of stress confinement: melting and resolidification of a surface region of the target, photomechanical spallation of a single or multiple layers or droplets, and an explosive disintegration of an overheated surface layer (phase explosion). The processes of laser melting, spallation, and phase explosion are taking place on the same time scale and are closely intertwined with each other. The transition to the spallation regime results in a reduction of the melting zone and a sharp drop in the duration of the melting and resolidification cycle. The transition from spallation to phase explosion is signified by an abrupt change in the composition of the ejected plume (from liquid layers and/or large droplets to a mixture of vapor-phase atoms, small clusters and droplets), and results in a substantial increase in the duration of the melting process. In simulations performed with longer, 50 ps, laser pulses, when the condition for stress confinement is not satisfied, the spallation regime is absent and phase explosion results in smaller values of the ablation yield and larger fractions of the vapor phase in the ejected plume as compared to the results obtained with a 1 ps pulse. The more vigorous material ejection and higher ablation yields, observed in the simulations performed with the shorter laser pulse, are explained by the synergistic contribution of the laser-induced stresses and the explosive release of vapor in phase explosion occurring under the condition of stress confinement.

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Femtosecond laser-induced cleaving of protein crystal in water solution

Cited by 9 publications

References 12 publications

Selective induction of targeted cell death and elimination by near-infrared femtosecond laser ablation

Selective induction of targeted cell death and elimination by near-infrared femtosecond laser ablation

Non-Photochemical Laser-Induced Nucleation of Sulfathiazole in a Water/Ethanol Mixture

Atomistic Modeling of Short Pulse Laser Ablation of Metals: Connections between Melting, Spallation, and Phase Explosion

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