Femtosecond laser nanofabrication of hydrogel biomaterial

Zhang, Wande; Chen, Shaochen

doi:10.1557/mrs.2011.275

Cited by 39 publications

(29 citation statements)

References 31 publications

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“…Recent developments in additive manufacturing technologies have enabled the construction of complex, precision-engineered 3D biomimetic scaffolds. 22 These methods include two-photon laser direct writing, 23 inkjet 3D printing, 24 extrusion based 3D printing, 25 and digital light projection (DLP) based 3D printing. 26,27 Two-photon laser direct writing can achieve 100 nm resolution, yet this process would take days to fabricate a millimeter-size structure due to its point-by-point writing process.…”

mentioning

confidence: 99%

^{A 3D Tissue-Printing Approach for Validation of Diffusion Tensor Imaging in Skeletal Muscle}

Berry

You

Warner

et al. 2017

Tissue Engineering Part A

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The ability to noninvasively assess skeletal muscle microstructure, which predicts function and disease, would be of significant clinical value. One method that holds this promise is diffusion tensor magnetic resonance imaging (DT-MRI), which is sensitive to the microscopic diffusion of water within tissues and has become ubiquitous in neuroimaging as a way of assessing neuronal structure and damage. However, its application to the assessment of changes in muscle microstructure associated with injury, pathology, or age remains poorly defined, because it is difficult to precisely control muscle microstructural features in vivo. However, recent advances in additive manufacturing technologies allow precision-engineered diffusion phantoms with histology informed skeletal muscle geometry to be manufactured. Therefore, the goal of this study was to develop skeletal muscle phantoms at relevant size scales to relate microstructural features to MRI-based diffusion measurements. A digital light projection based rapid 3D printing method was used to fabricate polyethylene glycol diacrylate based diffusion phantoms with (1) idealized muscle geometry (no geometry; fiber sizes of 30, 50, or 70 mm or fiber size of 50 mm with 40% of walls randomly deleted) or (2) histology-based geometry (normal and after 30-days of denervation) containing 20% or 50% phosphate-buffered saline (PBS). Mean absolute percent error (8%) of the printed phantoms indicated high conformity to templates when ''fibers'' were >50 mm. A multiple spin-echo echo planar imaging diffusion sequence, capable of acquiring diffusion weighted data at several echo times, was used in an attempt to combine relaxometry and diffusion techniques with the goal of separating intracellular and extracellular diffusion signals. When fiber size increased (30-70 mm) in the 20% PBS phantom, fractional anisotropy (FA) decreased (0.32-0.26) and mean diffusivity (MD) increased (0.44 · 10 -3 mm 2 /s-0.70 · 10 -3 mm 2 /s). Similarly, when fiber size increased from 30 to 70 mm in the 50% PBS diffusion phantoms, a small change in FA was observed (0.18-0.22), but MD increased from 0.86 · 10 -3 mm 2 /s to 1.79 · 10 -3 mm 2 /s. This study demonstrates a novel application of tissue engineering to understand complex diffusion signals in skeletal muscle. Through this work, we have also demonstrated the feasibility of 3D printing for skeletal muscle with relevant matrix geometries and physiologically relevant tissue characteristics.

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confidence: 99%

^{A 3D Tissue-Printing Approach for Validation of Diffusion Tensor Imaging in Skeletal Muscle}

Berry

You

Warner

et al. 2017

Tissue Engineering Part A

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“…Methacrylic acidizing and photo-cross-linking are used to form hydrogels and scaffold materials that are then used for tubulose construction printing of HA-gelatin hydrogels [82]. New semi-interpenetrating polymer networks (semi-IPNs) of dextran are derived from HA and ethoxyl metacrylic acid ester [113]. Furthermore, 24 nm gold nanoparticles (AuNPs) are dynamically cross-linked to HA modified with thiol and gelatin to form sECM hydrogels [114].…”

Section: Other Modifications Of Ha Hydrogelsmentioning

confidence: 99%

The Application of Hyaluronic Acid-Based Hydrogels in Bone and Cartilage Tissue Engineering

Zheng

et al. 2019

Advances in Materials Science and Engineering

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At present, the healing of osteopathy, especially the healing of cartilage, has been proven to be difficult. Commonly used treatment methods are autogenous bone grafts and allogeneic bone grafts, but grafts cannot fully meet the clinical treatment requirements due to problems related to the source, price, immunity, and other concerns. us, the combination of biomaterials and tissue engineering technology has become a new direction in research. Among studies on tissue engineering bone and cartilage materials, hydrogels that show biological activity, absorbability after degradability, plasticity, and easy preparation have become the focus. Hydrogels are used as extracellular matrix mimics. Although various materials are able to form hydrogels, hyaluronic acid and its derivatives are prominently used. Hyaluronic acid hydrogels have many advantages, such as promoting cell adhesion and proliferation and wound healing. ey also demonstrate sufficient biological activity for stimulating a microenvironment for cell survival. However, their disadvantages require further modification and include a poor degradation rate and insufficient mechanical performance. In this paper, hyaluronic acid-based hydrogels, their modifications, applications, and mechanisms, as well as new techniques for processing hyaluronic acid hydrogels in bone and cartilage tissue engineering, are briefly reviewed, and their future prospects and directions for future work are discussed.

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“…42 However, multi-photon polymerization requires the laser beam to pass through the entire photo-curable material without attenuation, which limits its application to only transparent materials, such as SU-8, 41 ORMOCERÒ, 42,43 ORMOCOMPÒ, 44 ORMOSILÒ, 45,46 zirconium based organic-inorganic hybrid material (SZ2080), [47][48][49] IP-L780 50 and biocompatible hydrogels. 51 With customsynthesized hybrid materials, 3D scaffolds can be fabricated with tunable geometries (e.g., porosity, pore size etc.) 48 and mechanical properties (e.g., stiffness).…”

Section: D Structure Fabrication Using Multi-photon Polymerizationmentioning

confidence: 99%

Laser-assisted biofabrication in tissue engineering and regenerative medicine

et al. 2016

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Controlling the spatial arrangement of biomaterials and living cells provides the foundation for fabricating complex biological systems. Such level of spatial resolution (less than 10 lm) is difficult to be obtained through conventional cell processing techniques, which lack the precision, reproducibility, automation, and speed required for the rapid fabrication of engineered tissue constructs. Recently, laserassisted biofabrication techniques are being intensively developed with the use of computer-aided processes for patterning and assembling both living and nonliving materials with prescribed 2D or 3D organization. In this review, we discuss laser-assisted fabrication methods, including laser tweezers, multi-photon polymerization, laser-induced forward transfer (LIFT), matrix assisted pulsed laser evaporation (MAPLE), and laser ablation as well as their applications in biological science and biomedical engineering. These advanced technologies enable the precise manipulation of in vitro cellular microenvironments and the ability to engineer functional tissue constructs with high complexity and heterogeneity, which serve in regenerative medicine, pharmacology, and basic cell biology studies.

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Femtosecond laser nanofabrication of hydrogel biomaterial

Abstract: Abstract

Cited by 39 publications

References 31 publications

^{A 3D Tissue-Printing Approach for Validation of Diffusion Tensor Imaging in Skeletal Muscle}

^{A 3D Tissue-Printing Approach for Validation of Diffusion Tensor Imaging in Skeletal Muscle}

The Application of Hyaluronic Acid-Based Hydrogels in Bone and Cartilage Tissue Engineering

Laser-assisted biofabrication in tissue engineering and regenerative medicine

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