FENDRR Sponges miR-424-5p to Inhibit Cell Proliferation, Migration and Invasion in Colorectal Cancer

Abstract

Objective: LncRNAs are non-coding RNAs exerting vital roles in the occurrence and development of various cancer types. This study tended to describe the expression pattern of FENDRR in colorectal cancer (CRC), and further investigate the role of FENDRR in CRC cell biological behaviors. Methods: Gene expression profile of colon cancer was accessed from the TCGA database, and then processed for differential analysis for identification of differentially expressed lncRNAs and miRNAs. Some in vitro experiments like qRT-PCR, MTT, colony formation assay, wound healing assay and Transwell assay were performed to assess the effect of FENDRR on cell biological behaviors. Dual-luciferase reporter assay was conducted to further validate the targeting relationship between FENDRR and miR-424-5p, and rescue experiments were carried out for determining the mechanism of FENDRR/miR-424-5p underlying the proliferation, migration and invasion of CRC cells. Results: Bioinformatics analysis suggested that FENDRR was significantly down-regulated in CRC tissue, and low FENDRR was intimately correlated to poor prognosis. FENDRR overexpression could greatly inhibit cell proliferation, migration and invasion. Besides, there was a negative correlation between FENDRR and miR-424-5p. Dual-luciferase reporter assay indicated that miR-424-5p was a direct target of FENDRR. Rescue experiments discovered that FENDRR exerted its role in cell proliferation, migration and invasion in CRC via targeting miR-424-5p. Conclusion: FENDRR is poorly expressed in CRC tissue and cells, and low FENDRR is responsible for the inhibition of cell proliferation, migration and invasion of CRC by means of targeting miR-424-5p.

Mechanism of miR‐424‐5p promoter methylation in promoting epithelial–mesenchymal transition of hepatocellular carcinoma cells

“…miRNAs are well‐established as a class of endogenous, noncoding single‐chained small RNAs whose differential expressions are closely associated with HCC progression 17 . One such miRNA, namely miR‐424‐5p, has been previously implicated in the regulation of malignant behaviors in a variety of carcinomas 18 . Accordingly, we collected a total of 30 pairs of HCC tissues and normal paracancerous tissues.…”

“…17 One such miRNA, namely miR-424-5p, has been previously implicated in the regulation of malignant behaviors in a variety of carcinomas. 18 Accordingly, we collected a total of 30 pairs of HCC tissues and normal paracancerous tissues. Subsequently, miR-424-5p expression patterns in human HCC cell lines (Huh7 and HepG) and immortalized normal hepatocytes L02 were determined by qRT-PCR (Figure 1A), which revealed that miR-424-5p expression levels were lower in both HCC tissues or cell lines than those in normal paracancerous tissues or L02 cells (p < 0.001).…”

Construction of immune-related lncRNA signature to predict aggressiveness, immune landscape, and drug resistance of colon cancer

“…Indeed, amassing studies have narrated the immune-related functions of some of the lncRNAs included in our risk model constructs. For example, FENDRR facilitates colorectal cancer growth and metastasis through interaction with miR-424-5p [ 22 ]. AC124067.4 was reported to affect the immunotherapy and prognosis outcomes of colon cancer patients via impacting genome instability [ 23 ].…”

A Novel Signature Constructed by Immune-Related LncRNA Predicts the Immune Landscape of Colorectal Cancer

“…In colorectal cancer, Yin et al (2019) revealed that FENDRR could inhibit tumor aggressiveness by regulating the miR-18a-5p/ING4 axis. Data from Cheng et al (2020) indicated that FENDRR could inhibit cell proliferation, migration, and invasion in CRC by targeting miR-424-5p. Liu and Du (2019) proved that FENDRR might work as a tumor suppressor gene in colon cancer by inhibiting SOX4.…”