FENDRR suppresses cervical cancer proliferation and invasion by targeting miR-15a/b-5p and regulating TUBA1A expression

Zhu, Yunheng; Zhang, Xiaohua; Wang, Lifeng; Zhu, Xiuxiang; Xia, Ziyin; Xu, Ling; Xu, Jun

doi:10.1186/s12935-020-01223-w

Cited by 24 publications

(18 citation statements)

References 22 publications

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“…A similar story emerged when we compared lncRNA enriched with SFPQ in PM and melanoma cell backgrounds. The SFPQenriched lncRNA transcripts most specific to PM were EMX2OS and FENDRR, the latter reported to function as a tumour suppressor in most cancers, including melanoma [27,[46][47][48][49]. The function of EMX2OS in cancer differs depending on cell background [28,50].…”

Section: Discussionmentioning

confidence: 99%

SFPQ promotes an oncogenic transcriptomic state in melanoma

Anene

Nsengimana

et al. 2021

Oncogene

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The multifunctional protein, splicing factor, proline- and glutamine-rich (SFPQ) has been implicated in numerous cancers often due to interaction with coding and non-coding RNAs, however, its role in melanoma remains unclear. We report that knockdown of SFPQ expression in melanoma cells decelerates several cancer-associated cell phenotypes, including cell growth, migration, epithelial to mesenchymal transition, apoptosis, and glycolysis. RIP-seq analysis revealed that the SFPQ-RNA interactome is reprogrammed in melanoma cells and specifically enriched with key melanoma-associated coding and long non-coding transcripts, including SOX10, AMIGO2 and LINC00511 and in most cases SFPQ is required for the efficient expression of these genes. Functional analysis of two SFPQ-enriched lncRNA, LINC00511 and LINC01234, demonstrated that these genes independently contribute to the melanoma phenotype and a more detailed analysis of LINC00511 indicated that this occurs in part via modulation of the miR-625-5p/PKM2 axis. Importantly, analysis of a large clinical cohort revealed that elevated expression of SFPQ in primary melanoma tumours may have utility as a prognostic biomarker. Together, these data suggest that SFPQ is an important driver of melanoma, likely due to SFPQ–RNA interactions promoting the expression of numerous oncogenic transcripts.

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Section: Discussionmentioning

confidence: 99%

SFPQ promotes an oncogenic transcriptomic state in melanoma

Anene

Nsengimana

et al. 2021

Oncogene

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“…According to the competitive endogenous RNA (ceRNA) mechanism, endogenous lncRNAs with miRNA target sites have the potential to act as natural miRNA sponges, which suppress the expression of miRNA targets by competitively binding and inhibiting miRNAs ( 40 ). Notably, some lncRNAs (PVT1 and FENDRR) have been identified to regulate miR-15a-5p expression via the ceRNA network ( 41 , 42 ). The current data identified potential crosstalk between ANRIL and miR-15a-5p, suggesting that ANRIL knockdown inhibited the progression of AR by preventing the sponging of miR-15a-5p.…”

Section: Discussionmentioning

confidence: 99%

Knockdown of lncRNA ANRIL suppresses the production of inflammatory cytokines and mucin 5AC in nasal epithelial cells via the miR-15a-5p/JAK2 axis

Liu

et al. 2020

Mol Med Rep

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The incidence of allergic rhinitis (AR) is increasing worldwide. Human nasal epithelial cells (HNECs) are the key cells in the occurrence of AR. Antisense non-coding RNA in the INK4 locus (ANRIL) was discovered to be involved in the progression of AR. However, the mechanism by which ANRIL mediates the progression of AR remains to be determined. The present study aimed to further explore the mechanism by which ANRIL regulates AR. Thereby, HNECs were treated with IL-13 to mimic AR in vitro . The mRNA expression levels of ANRIL, microRNA (miR)-15a-5p, JAK2, mucin 5AC (MUC5AC), granulocyte-macrophage colony-stimulating factor (GM-CSF) and eotaxin-1, and protein expression levels of JAK2, STAT3 and phosphorylated-STAT3 in HNECs were analyzed using reverse transcription-quantitative PCR and western blotting, respectively. ELISAs were used to detect the secretory levels of inflammatory cytokines and mucin in cell supernatants. In addition, a dual luciferase reporter assay was used to confirm the downstream target of ANRIL and the target gene of miR-15a-5p. The results revealed that the secretory levels of eotaxin-1, GM-CSF and MUC5AC were significantly upregulated by IL-13 in the supernatant of HNECs. The expression levels of ANRIL and JAK2 were also upregulated in IL-13-induced HNECs, while the expression levels of miR-15a-5p were downregulated. In addition, ANRIL was identified to bind to miR-15a-5p. The IL-13-induced upregulation of eotaxin-1, GM-CSF and MUC5AC mRNA expression and secretory levels was significantly inhibited by the genetic knockdown of ANRIL, while the miR-15a-5p inhibitor effectively reversed this effect. JAK2 was also discovered to be directly targeted by miR-15a-5p. The overexpression of JAK2 significantly suppressed the therapeutic effect of miR-15a-5p mimics on IL-13-induced inflammation in vitro . In conclusion, the findings of the present study suggested that the genetic knockdown of ANRIL may suppress the production of inflammatory cytokines and mucin in IL-13-treated HNECs via regulation of the miR-15a-5p/JAK2 axis. Thus, ANRIL may serve as a novel target for AR treatment.

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“…Moreover, FENDRR takes effect in different cancers, such as hepatocellular carcinoma, cholangiocarcinoma, gastric cancer, cervical cancer, breast cancer, prostate cancer, endometrial cancer, and non-small cell lung cancer ( He et al, 2018 ; Li et al, 2018 ; Qin et al, 2019 ; Yu et al, 2019 ; Zhang G. et al, 2019 ; Zhang Y.Q. et al, 2019 ; Zhu et al, 2020 ). It can be seen that FENDRR is closely involved in the process of tumors related to the digestive system and reproductive system.…”

Section: Discussionmentioning

confidence: 99%

A Novel Signature Constructed by Immune-Related LncRNA Predicts the Immune Landscape of Colorectal Cancer

et al. 2021

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Colorectal cancer (CRC) has the characteristics of high morbidity and mortality. LncRNA not only participates in the progression of CRC through genes and transcription levels, but also regulates the tumor microenvironment and leads to the malignant phenotype of tumors. Therefore, we identified immune-related LncRNAs for the construction of clinical prognostic model. We searched The Cancer Genome Atlas (TCGA) database for original data. Then we identified differentially expressed irlncRNA (DEirlncRNA), which was paired and verified subsequently. Next, univariate analysis, Lasso and Cox regression analysis were performed on the DEirlncRNA pair. The ROC curve of the signature was drawn, and the optimal cut-off value was found. Then the cohort was divided into a high-risk and a low-risk group. Finally, we re-evaluated the signature from different perspectives. A total of 16 pairs of DEirlncRNA were included in the construction of the model. After regrouping according to the cut-off value of 1.275, the high-risk group showed adverse survival outcomes, progressive clinicopathological features, specific immune cell infiltration status, and high sensitivity to some chemotherapy drugs. In conclusion, we constructed a signature composed of immune-related LncRNA pair with no requirement of the specific expression level of genes, which shows promising clinical predictive value in CRC patients.

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FENDRR suppresses cervical cancer proliferation and invasion by targeting miR-15a/b-5p and regulating TUBA1A expression

Cited by 24 publications

References 22 publications

SFPQ promotes an oncogenic transcriptomic state in melanoma

SFPQ promotes an oncogenic transcriptomic state in melanoma

Knockdown of lncRNA ANRIL suppresses the production of inflammatory cytokines and mucin 5AC in nasal epithelial cells via the miR-15a-5p/JAK2 axis

A Novel Signature Constructed by Immune-Related LncRNA Predicts the Immune Landscape of Colorectal Cancer

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