Fengycin antibiotics isolated from B-FS01 culture inhibit the growth ofFusarium moniliformeSheldon ATCC 38932

Hu, Liangbin; Shi, Zhi Qi; Zhang, Ting; Yang, Zhi Min

doi:10.1111/j.1574-6968.2007.00743.x

Cited by 92 publications

(68 citation statements)

References 44 publications

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“…The hazy zones indicated an intermediate inhibition and induction of early sporulation due to activation of stress response in presence of APB. These variations in inhibition patterns may be attributed to interaction of lipopeptides with sterol molecules in cell membrane, which vary in different groups of fungi (Hu et al, 2007). This was further confirmed by light and scanning electron microscopy, as Fig.…”

Section: Discussionsupporting

confidence: 59%

Antifungal potential of Bacillus vallismortis R2 against different phytopathogenic fungi

Kaur

Saini

2015

Span. j. agric. res.

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The cash crops grown in an agro-climatic region are prone to infection by various fungal pathogens. The use of chemical fungicides over the years has resulted in emergence of resistant fungal strains, thereby necessitating the development of effective and environmental friendly alternatives. The natural antagonistic interactions among different microbial populations have been exploited as an eco-friendly approach for controlling fungal pathogens resistant to synthetic chemicals. Morphologically distinct bacterial cultures (150), isolated from rhizospheric soils of wheat, rice, onion and tomato plants were screened for their antifungal potential against seven phytopathogenic fungi prevalent in the State of Punjab (India). The bacterial isolate R2, identified as Bacillus vallismortis, supported more than 50% inhibition of different phytopathogenic fungi (Alternaria alternata, Rhizoctonia oryzae, Fusarium oxysporum, Fusarium moniliforme, Colletotrichum sp, Helminthosporium sp and Magnaporthe grisea) in dual culture plate assay. The thin layer chromatography based bio-autography of acid-precipitated biomolecules (APB) indicated the presence of more than one type of antifungal molecule, as evidenced from zones of inhibition against the respective fungal pathogen. The initial analytical studies indicated the presence of surfactin, iturin A and fengycin-like compounds in APB. The antifungal activity of whole cells and APB of isolate R2 was evaluated by light and scanning electron microscopy. The wheat grains treated with APB and exposed to spores of A. alternata showed resistance to the development of black point disease, thereby indicating the potential application of R2 and its biomolecules at field scale level.

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Section: Discussionsupporting

confidence: 59%

Antifungal potential of Bacillus vallismortis R2 against different phytopathogenic fungi

Kaur

Saini

2015

Span. j. agric. res.

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“…The fragmentation profile of the ions of m/z 1,463.8, 1,435.7, and 1,449.9 contained product ions of m/z 966 and 1,080. This pair of ions was described previously by Bie et al [4] and Hu et al [14] as those originating from fengycin A and can be explained by neutral losses of (fatty acid-Glu) and (fatty acid-Glu-Orn) from the N-terminal segment, respectively. Figure 4 shows the pair of ions of m/z 966 and 1,080 from the fragmentation of the ion of m/z 1,463.8.…”

Section: Mass Spectrometrymentioning

confidence: 75%

“…Fengycins represent the third family of Bacillus lipopeptide surfactants and, including the related plipastatin. These surfactants are decapeptides containing ornithine attached to a b-hydroxy fatty acid, and in addition to surfactant activity also show powerful antimicrobial activity, particularly against filamentous fungi, making them of interest to agriculture industry for use in the control of plant pathogens [14,33].…”

Section: Introductionmentioning

confidence: 99%

Purification and structural characterization of fengycin homologues produced by Bacillus subtilis LSFM-05 grown on raw glycerol

Faria

Stéfani

Vaz

et al. 2011

J Ind Microbiol Biotechnol

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Raw glycerol is a byproduct of biodiesel production that currently has low to negative value for biodiesel producers. One option for increasing the value of raw glycerol is to use it as a feedstock for microbial production. Bacillus subtilis LSFM 05 was used for the production of fengycin in a mineral medium containing raw glycerol as the sole carbon source. Fengycin was isolated by acid precipitation at pH 2 and purified by silica gel column chromatography and characterized using electrospray ionization (ESI) Fourier transform ion cyclotron resonance mass spectrometry (ESI FT-ICR MS) with collision-induced dissociation (CID). The mass spectrum revealed the presence of the ions of m/z 1,435.7, 1,449.9, 1,463.8, 1,477.8, 1,491.8 and 1,505.8, which were further fragmented by ESI-MS/MS. The CID profile showed the presence of a series of ions (m/z 1,080 and 966) and (m/z 1,108 and 994) that represented the different fengycin homologues A and B, respectively. Fengycin homologues A and B are variants that differ at position 6 of the peptide moiety, having either Ala or Val residues, respectively. Mass spectrometry analyses identified four fengycin A and three fengycin B variants with fatty acid components containing 14-17 carbons. These results demonstrate that raw glycerol can be used as feedstock to produce fengycin, and additional work should focus on the optimization of process conditions to increase productivity.

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“…MALDI-TOF-MS analyses were reported by Williams et al [23] and Kim et al [24] to characterise fengycins obtained from Bacillus globigii and from Bacillusthuringiensis. Electrospray ionisation (ESI) tandem mass spectrometry was reported to be able to distinguish between different fengycin homologues produced by Bacillus subtilis strains, [17,25] or to sequence the peptidic moiety of natural and hydrolysed fengycins. [19] Recently, Chen et al [26] reported the use of this technique to characterise the lipopeptides produced by B. subtilis JA.…”

Section: Introductionmentioning

confidence: 99%

LC/ESI‐MS/MS characterisation of lipopeptide biosurfactants produced by the Bacillus licheniformis V9T14 strain

et al. 2010

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Lipopeptide biosurfactants produced by the Bacillus licheniformis V9T14 strain showed an interesting anti-adhesion activity against biofilm formation of human pathogenic bacterial strains. The chemical characterisation of the crude extract of V9T14 strain was first developed through electrospray ionisation mass spectrometry (ESI-MS) and ESI-MS/MS direct infusions: two sets of molecular ion species belonging to the fengycin and surfactin families were revealed and their structures defined, interpreting their product ion spectra. The LC/ESI-MS analysis of the crude extract allowed to separate in different chromatogram ranges the homologues and the isoforms of the two lipopeptide families. The extract was then fractionated by silica gel chromatography in two main fractions, I and II. The purified biosurfactants were analysed through a new, rapid and suitable LC/ESI-MS/MS method, which allowed characterising the composition and the structures of the produced lipopeptides. LC/ESI-MS/MS analysis of fraction I showed the presence of C(13), C(14) and C(15) surfactin homologues, whose structures were confirmed by the product ion spectra of the sodiated molecules [M + Na](+) at m/z 1030, 1044 and 1058. LC/ESI-MS/MS analysis of fraction II confirmed the presence of two main fengycin isoforms, with the protonated molecules [M + H](+) at m/z 1478 and 1506 corresponding to C(17) fengycin A and C(17) fengycin B, respectively. Other homologues (C(14) to C(16)) were revealed and confirmed as belonging to fengycin A or B according to the retention times and the product ions generated, although with the same nominal mass. Finally, a relative percentage content of each homologue for both lipopeptides families in the whole extract was proposed.

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Fengycin antibiotics isolated from B-FS01 culture inhibit the growth ofFusarium moniliformeSheldon ATCC 38932

Cited by 92 publications

References 44 publications

Antifungal potential of Bacillus vallismortis R2 against different phytopathogenic fungi

Antifungal potential of Bacillus vallismortis R2 against different phytopathogenic fungi

Purification and structural characterization of fengycin homologues produced by Bacillus subtilis LSFM-05 grown on raw glycerol

LC/ESI‐MS/MS characterisation of lipopeptide biosurfactants produced by the Bacillus licheniformis V9T14 strain

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