Fengycins From Bacillus amyloliquefaciens MEP218 Exhibit Antibacterial Activity by Producing Alterations on the Cell Surface of the Pathogens Xanthomonas axonopodis pv. vesicatoria and Pseudomonas aeruginosa PA01

Medeot, Daniela B.; Fernandez, Maricruz; Morales, Gustavo M.; Jofré, Edgardo

doi:10.3389/fmicb.2019.03107

Cited by 60 publications

(31 citation statements)

References 63 publications

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“…Basit et al [ 56 ] isolated 3 strains of Bacillus cereus from garden soil whose lipopeptide biosurfactants exhibited significant antibacterial and antifungal activity against S. aureus , Escherichia coli , P. aeruginosa , K. pneumoniae , Aspergillus niger and Candida albicans , with MIC values ranging from 0.52 to 7.6 mg/mL. More recently, Medeot et al [ 57 ] demonstrated that fengycin form Bacillus amyloliquefaciens MEP218 was able to induce dramatic alterations in the surface topography of the opportunistic human pathogen P. aeruginosa PA01, leading to a decrease in cell height and loss in intracellular content. The surfactin and rhamnolipids mixtures produced by B. amyloliquefaciens ST34 and P. aeruginosa ST5, respectively, showed a pronounced antimicrobial activity against a broad spectrum of opportunistic and pathogenic microorganisms, including antibiotic-resistant bacterial strains, such as S. aureus and E. coli and the yeast C. albicans [ 58 ].…”

Section: Antimicrobial Activity Of Bssmentioning

confidence: 99%

Recent Advances in Biomedical, Therapeutic and Pharmaceutical Applications of Microbial Surfactants

et al. 2021

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The spread of antimicrobial-resistant pathogens typically existing in biofilm formation and the recent COVID-19 pandemic, although unrelated phenomena, have demonstrated the urgent need for methods to combat such increasing threats. New avenues of research for natural molecules with desirable properties to alleviate this situation have, therefore, been expanding. Biosurfactants comprise a group of unique and varied amphiphilic molecules of microbial origin capable of interacting with lipidic membranes/components of microorganisms and altering their physicochemical properties. These features have encouraged closer investigations of these microbial metabolites as new pharmaceutics with potential applications in clinical, hygiene and therapeutic fields. Mounting evidence has indicated that biosurfactants have antimicrobial, antibiofilm, antiviral, immunomodulatory and antiproliferative activities that are exploitable in new anticancer treatments and wound healing applications. Some biosurfactants have already been approved for use in clinical, food and environmental fields, while others are currently under investigation and development as antimicrobials or adjuvants to antibiotics for microbial suppression and biofilm eradication strategies. Moreover, due to the COVID-19 pandemic, biosurfactants are now being explored as an alternative to current products or procedures for effective cleaning and handwash formulations, antiviral plastic and fabric surface coating agents for shields and masks. In addition, biosurfactants have shown promise as drug delivery systems and in the medicinal relief of symptoms associated with SARS-CoV-2 acute respiratory distress syndrome.

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Section: Antimicrobial Activity Of Bssmentioning

confidence: 99%

Recent Advances in Biomedical, Therapeutic and Pharmaceutical Applications of Microbial Surfactants

et al. 2021

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“…For instance, the bactericidal effect of fengycins from B. amyloliquefaciens MEP218 on X. axonopodis pv. vesicatoria and P. aeruginosa PA01 was documented by Medeot et al [ 27 ]. Our analyses showed that the 13 ionic forms belong to the lipopeptide fengycin ( Supplementary Table S1 ).…”

Section: Resultsmentioning

confidence: 99%

“…The other two lipopeptides, fengycin and bacillomycin, were produced in the dual culture at respective maximal concentrations of 2.0 ± 0.4 µg/mL and 1.6 ± 0.3 µg/mL. Purified bacillomycin and fengycin lipopeptides were shown to exhibit the antibiotic activity in the range of concentrations of 2–32 µg/mL and 6.25 µg/mL, respectively [ 27 , 52 ]. The reason for low concentration of metabolites could be a competition for nutrients between the two microorganisms during growth.…”

Section: Resultsmentioning

confidence: 99%

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Killing Effect of Bacillus velezensis FZB42 on a Xanthomonas campestris pv. Campestris (Xcc) Strain Newly Isolated from Cabbage Brassica oleracea Convar. Capitata (L.): A Metabolomic Study

et al. 2021

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The potential use of Bacillus velezensis FZB42 for biological control of various phytopathogens has been documented over the past few years, but its antagonistic interactions with xanthomonads has not been studied in detail. Novel aspects in this study consist of close observation of the death of Xanthomonas campestris pv. campestris cells in a co-culture with B. velezensis FZB42, and quantification of lipopeptides and a siderophore, bacillibactin, involved in the killing process. A new robust Xcc-SU isolate tolerating high concentrations of ferric ions was used. In a co-culture with the antagonist, the population of Xcc-SU was entirely destroyed within 24–48 h, depending on the number of antagonist cells used for inoculation. No inhibitory effect of Xcc-SU on B. velezensis was observed. Bacillibactin and lipopeptides (surfactin, fengycin, and bacillomycin) were present in the co-culture and the monoculture of B. velezensis. Except for bacillibactin, the maximum contents of lipopeptides were higher in the antagonist monoculture compared with the co-culture. Scanning electron microscopy showed that the death of Xcc-SU bacteria in co-culture was caused by cell lysis, leading to an enhanced occurrence of distorted cells and cell ghosts. Analysis by mass spectrometry showed four significant compounds, bacillibactin, surfactin, fengycin, and bacillomycin D amongst a total of 24 different forms detected in the co-culture supernatant: Different forms of surfactin and fengycin with variations in their side-chain length were also detected. These results demonstrate the ability of B. velezensis FZB42 to act as a potent antagonistic strain against Xcc.

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“…Recent study has confirmed antibacterial effect of fengycins against Xanthomonas axonopodis pv. vesicatoria by causing alterations on the cell surface and loss of intracellular content [ 51 ]. On the other hand, lipopeptide putatively identified as locillomycin was detected at m / z value of 1146.8, representing a relatively novel family of lipopeptides which exhibit antibacterial and antiviral activity, but also limited antifungal activity [ 45 , 52 ].…”

Section: Discussionmentioning

confidence: 99%

Pepper Bacterial Spot Control by Bacillus velezensis: Bioprocess Solution

et al. 2020

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Pepper bacterial spot is one of the most severe plant diseases in terms of infection persistence and economic losses when it comes to fresh pepper fruits used in nutrition and industrial processing. In this study, Bacillus velezensis IP22 isolated from fresh cheese was used as a biocontrol agent of pepper bacterial spot, whose main causal agent is the cosmopolitan pathogen Xanthomonas euvesicatoria. After optimization of the cultivation medium composition aimed at maximizing of the antimicrobial activity against X. euvesicatoria and validation of the optimized medium at the scale of a laboratory bioreactor, in planta tests were performed. The results have showed significant suppression of bacterial spot symptoms in pepper plants by the produced biocontrol agent, as well as reduction of disease spreading on the healthy (uninoculated) pepper leaves. Furthermore, HPLC-MS (high pressure liquid chromatography–mass spectrometry) analysis was employed to examine antimicrobial metabolites produced by B. velezensis IP22, where lipopeptides were found with similar m/z values compared to lipopeptides from fengycin and locillomycin families. The bioprocess solution developed at the laboratory scale investigated in this study represents a promising strategy for production of pepper bacterial spot biocontrol agent based on B. velezensis IP22, a food isolate with a great perspective for application in plant protection.

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Fengycins From Bacillus amyloliquefaciens MEP218 Exhibit Antibacterial Activity by Producing Alterations on the Cell Surface of the Pathogens Xanthomonas axonopodis pv. vesicatoria and Pseudomonas aeruginosa PA01

Cited by 60 publications

References 63 publications

Recent Advances in Biomedical, Therapeutic and Pharmaceutical Applications of Microbial Surfactants

Recent Advances in Biomedical, Therapeutic and Pharmaceutical Applications of Microbial Surfactants

Killing Effect of Bacillus velezensis FZB42 on a Xanthomonas campestris pv. Campestris (Xcc) Strain Newly Isolated from Cabbage Brassica oleracea Convar. Capitata (L.): A Metabolomic Study

Pepper Bacterial Spot Control by Bacillus velezensis: Bioprocess Solution

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