Fenhexamid Resistance in the Botrytis Species Complex, Responsible for Grey Mould Disease

Billard, Alexis; Fillinger, Sabine; Leroux, Pierre; Bach, Jocelyne; Lanen, Catherine; Lachaise, H.; Beffa, Roland; Debieu, Danièle

doi:10.5772/27512

Cited by 12 publications

(21 citation statements)

References 45 publications

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“…In 12 representative strains of this kind, the EC 50 values for germ-tube extension were within the same range (i.e. < 0.2 ppm) as those previously determined for Hyd R1 and Hyd R2 (Weber 2010a) and for non-metabolizing strains with baseline sensitivity (Suty et al 1999, Markoglou & Ziogas 2002, Weber 2010a, Billard et al 2011, Weber & Hahn 2011. Since metabolism of fenhexamid by Hyd R1 and Hyd R2 strains is dependent upon available carbon sources, this kind of response is readily detected in laboratory tests but is considered not to be relevant in the field (Leroux et al 2002, Leroux 2007.…”

Section: Discussionsupporting

confidence: 52%

“…The more common phenotype showed a retarded and morphologically aberrant growth at 1 and even 50 ppm fenhexamid in MEA, whilst being subject to complete inhibition at 10 ppm fenhexamid in TWA. This, together with substantial mycelial growth at 10 ppm on MEA, was indicative of fenhexamid metabolism of the Hyd R1 or Hyd R2 type (Weber 2010a, Billard et al 2011. In 12 representative strains of this kind, the EC 50 values for germ-tube extension were within the same range (i.e.…”

Section: Discussionmentioning

confidence: 61%

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Fungicide resistance of Botrytis cinerea on lettuce in Northern Germany

Weber

Wichura

2013

J Plant Dis Prot

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In 2010-2012, a total of 309 isolates of Botrytis cinerea from 23 lettuce fields in Northern Germany was examined for sensitivity to seven fungicides with specific modes of action. Resistance against the frequently used compounds boscalid (45.0% of all isolates), iprodione (37.2%) and quinone-outside inhibitors (strobilurins, 56.3%) was the most common. Isolates combining resistance to two or all three of these fungicides were more frequent than those showing resistance against only one. Strains with cyprodinil (31.4%) or thiophanate-methyl (11.6%) resistance were also observed in the majority of fields. In contrast, resistance against fludioxonil (1 isolate) and fenhexamid (3 isolates) was rare. The abundance of B. cinerea strains with multiple resistance to several currently used fungicides has implications for future lettuce crop management which are discussed.Abbrevations: HR = high resistance, MBC = methyl benzimidazole carbamate, MEA = malt extract agar, MR = moderate resistance, PDA = potato dextrose agar, QoI = quinone outside inhibitor, s = reduced sensitivity, SA = sucrose agar, ss = high sensitivity, TWA = tap water agar, YEA = yeast extract agar

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Section: Discussionsupporting

confidence: 52%

Section: Discussionmentioning

confidence: 61%

Fungicide resistance of Botrytis cinerea on lettuce in Northern Germany

Weber

Wichura

2013

J Plant Dis Prot

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“…Similarly, Billard et al 45 obtained the transgenic Bcerg27 L195F and Bcerg27 V309M mutants by gene replacement, and found that the fenhexamid resistance of the generated mutants was similar to that from the HydR3 − field strains. However, Fillinger et al 16 reported that the transgenic TELerg L400S , TELerg N369D , TELerg S336C , TELerg S336C,N369D and TELerg L195F mutants obtained by the insertion of replicative plasmid exhibited similar sensitivity to fenhexamid compared with the wild-type parent strain.…”

Section: Discussionmentioning

confidence: 99%

“…The codons V365, E368, E375 and A378, as well as codon N369, are near to the C-terminal putative transmembrane domain of erg27 which was found to play a role in 3-ketoreductase activity. 44,45 The amino acid leucine at position 269 is also present in the erg27 protein in B. pseudocinerea, which carries 12 amino acid residues different from those of the sensitive B. cinerea strain. 15 Amino acids P57 and S9 are located in domains of still unknown function.…”

Section: Discussionmentioning

confidence: 99%

“…As for mutations related to high resistance, the transformants Bcerg27 F412S , Bcerg27 F412I and Bcerg27 F412V all presented high resistance levels, as the HydR3 + field strains did. 16,45 The widespread occurrence of multifungicide-resistant populations of Botrytis seriously threatens effective management of grey mould. Our previous study found that 52.3% of grey mould populations from strawberry in Zhejiang Province are simultaneously resistant to azoxystrobin, carbendazim, iprodione and pyrimethanil.…”

Section: Discussionmentioning

confidence: 99%

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The natural fenhexamid‐resistant grey mould populations from strawberry in Zhejiang Province are dominated by Botrytis cinerea group S

Yin

Liu

et al. 2015

Pest Management Science

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Fungicide resistance characterized across seven modes of action in Botrytis cinerea isolated from Australian vineyards

Harper

Paton

Hall

et al. 2021

Pest Management Science

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BACKGROUND: Botrytis bunch rot, caused by Botrytis cinerea, is an economically important disease of grapes in Australia and across grape-growing regions worldwide. Control of this disease relies on canopy management and the application of fungicides. Fungicide application can lead to the selection of resistant B. cinerea populations, which has an adverse effect on the management of the disease. Characterizing the distribution and severity of resistant B. cinerea populations is needed to inform resistance management strategies.RESULTS: In this study, 724 isolates were sampled from 76 Australian vineyards during 2013-2016 and were screened against seven fungicides with different modes of action (MOAs). The resistance frequencies for azoxystrobin, boscalid, fenhexamid, fludioxonil, iprodione, pyrimethanil and tebuconazole were 5%, 2.8%, 2.1%, 6.2%, 11.6%, 7.7% and 2.9%, respectively. Nearly half of the resistant isolates (43.8%) were resistant to more than one of the fungicides tested. The frequency of vineyards with at least one isolate simultaneously resistant to one, two, three, four or five fungicides was 19.7%, 7.9%, 6.6%, 10.5% and 2.6%. Resistance was associated with previously published genotypes in CytB (G143A), SdhB (H272R/Y), Erg27 (F412S), Mrr1 (D354Y), Bos1 (I365S, N373S + Q369P, I365S + D757N) and Pos5 (V273I, P319A, L412F/V). Novel genotypes were also described in Mrr1 (S611N, D616G), Pos5 (V273L) and Cyp51 (P347S). Expression analysis was used to characterize fludioxonil-resistant isolates exhibiting overexpression (6.3-9.6-fold) of the ABC transporter gene AtrB (MDR1 phenotype).CONCLUSION: Resistance frequencies were lower when compared to most previously published surveys of B. cinerea resistance in grape and other crops. Nevertheless, continued monitoring of critical MOAs used in Australian vineyards is recommended.

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Fenhexamid Resistance in the Botrytis Species Complex, Responsible for Grey Mould Disease

Cited by 12 publications

References 45 publications

Fungicide resistance of Botrytis cinerea on lettuce in Northern Germany

Fungicide resistance of Botrytis cinerea on lettuce in Northern Germany

The natural fenhexamid‐resistant grey mould populations from strawberry in Zhejiang Province are dominated by Botrytis cinerea group S

Fungicide resistance characterized across seven modes of action in Botrytis cinerea isolated from Australian vineyards

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