FENILPROPANOIDES COM AÇÃO ANTI-Trypanosoma cruzi ISOLADOS DE Baccharis ligustrina C. DC. (ASTERACEAE)

Abstract

ANTI-Trypanosoma cruzi PHENYLPROPANOIDS ISOLATED FROM Baccharis ligustrina C. DC. (ASTERACEAE). In the present work, dried aerial parts of Baccharis ligustrina (Asteraceae) were subjected to microwave assisted extraction (MAE) using aqueous solution of 1-butyl-3-methylimidazolium bromide (BMImBr) and the obtained extract was successively partitioned using hexane and EtOAc. Using reduced amounts of extracts and efficient chromatographic steps, four acyl C6C3 derivatives (n-hexacosyl ferulate, n-hexacosyl, n-octacosyl, and n-triacontyl p-coumarates) were obtained from hexane phase whereas two C6C3 acids (ferulic and p-coumaric) were obtained from EtOAc phase. Isolated phenylpropanoids were evaluated against amastigote forms of parasite Trypanosoma cruzi. As result, it was observed that p-coumaric and ferulic acids were inactives whereas alkyl derivatives displayed EC50 values of 6.5 µmol L-1 (n-octacosyl p-coumarate), 9.3 µmol L-1 (n-triacontyl p-coumarate), 15.7 µmol L-1 (n-hexacosyl p-coumarate), and 32.2 µmol L-1 (n-hexacosyl ferulate). All tested compounds displayed reduced toxicity against NCTC cells (CC50 > 200 mmol L-1).

Evaluation of Anti-Trypanosoma cruzi Activity of Chemical Constituents from Baccharis sphenophylla Isolated Using High-Performance Countercurrent Chromatography

“…This could be a result of denaturation of the compound after penetration into the host cell or a lack of penetration in the macrophages. Compounds containing a hydroxycinnamic group analogue to that found in compound 2 are known to exhibit anti-T. cruzi activity, such as the phenylpropanoids isolated from B. sphenophylla [23] and B. ligustrina [36]. Compound 3 displayed moderate activity against trypomastigote forms, with an EC 50 value of 10.6 µM, but it was inactive against the intracellular amastigotes.…”

Highly selective alkylcoumarates and diterpenoids isolated from leaves of Baccharis quitensis : a search for new hit compounds against Trypanosoma cruzi

“…At the end of the assay, slides were fixed with MeOH and stained with Giemsa prior to counting under a light microscope (EVOS M5000, Thermo). EC50 values were calculated as previously described (Silva et al 2023).…”