Fenugreek (Trigonellafoenum-graecum L.) Seeds Dietary Supplementation Regulates Liver Antioxidant Defense Systems in Aging Mice

Tewari, Devesh; Jóźwik, Artur; Łysek-Gładysińska, Małgorzata; Grzybek, Weronika; Adamus-Białek, Wioletta; Bicki, Jacek; Strzałkowska, N.; Kamińska, Agnieszka; Horbańczuk, Olaf K.; Atanasov, A

doi:10.20944/preprints202007.0146.v1

Cited by 6 publications

(1 citation statement)

References 20 publications

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“…The antidiabetic property of 4-HIL is attributed to its ability to induce insulin secretion in a glucose-dependent manner 12,13 . There are several other important classes of metabolites such as volatiles (eugenol, linalool), avonoids (quercetin, rutin, vitexin and isovitexin) which render fenugreek multiple medicinal properties 7,14 .…”

Section: Introductionmentioning

confidence: 99%

Exploring the Medicinally Important Scondary Metabolites Landscape Through the Lens of Transcriptome Data in Fenugreek (Trigonella Foenum Graecum L.)

Naika

Sathyanarayanan

Sajeevan

et al. 2022

Preprint

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Fenugreek (Trigonella foenum-graecum L.) is a self-pollinated leguminous crop belonging to the Fabaceae family. It is a multipurpose crop used as herb, spice, vegetable and forage. It is a traditional medicinal plant in India attributed with several nutritional and medicinal properties including antidiabetic and anticancer. We have performed a combined transcriptome assembly from RNA sequencing data derived from leaf, stem and root tissues. Around 2,09,831 transcripts were deciphered from the assembly of 92% completeness and an N50 of 1382 bases. Whilst secondary metabolites of medicinal value, such as trigonelline, diosgenin, 4-hydroxyisoleucine and quercetin, are distributed in several tissues, we report transcripts that bear sequence signatures of enzymes involved in the biosynthesis of such metabolites and are highly expressed in leaves, stem and roots. One of the antidiabetic alkaloid, trigonelline and its biosynthesising enzyme, is highly abundant in leaves. These findings are of value to nutritional and the pharmaceutical industry.

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Section: Introductionmentioning

confidence: 99%

Exploring the Medicinally Important Scondary Metabolites Landscape Through the Lens of Transcriptome Data in Fenugreek (Trigonella Foenum Graecum L.)

Naika

Sathyanarayanan

Sajeevan

et al. 2022

Preprint

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Aging-Related Changes in the Ultrastructure of Hepatocytes and Cardiomyocytes of Elderly Mice Are Enhanced in ApoE-Deficient Animals

Łysek-Gładysińska

Wieczorek

Jóźwik

et al. 2021

Cells

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Biological aging is associated with various morphological and functional changes, yet the mechanisms of these phenomena remain unclear in many tissues and organs. Hyperlipidemia is among the factors putatively involved in the aging of the liver and heart. Here, we analyzed morphological, ultrastructural, and biochemical features in adult (7-month-old) and elderly (17-month-old) mice, and then compared age-related features between wild type (C57Bl/6 strain) and ApoE-deficient (transgenic ApoE−/−) animals. Increased numbers of damaged mitochondria, lysosomes, and lipid depositions were observed in the hepatocytes of elderly animals. Importantly, these aging-related changes were significantly stronger in hepatocytes from ApoE-deficient animals. An increased number of damaged mitochondria was observed in the cardiomyocytes of elderly animals. However, the difference between wild type and ApoE-deficient mice was expressed in the larger size of mitochondria detected in the transgenic animals. Moreover, a few aging-related differences were noted between wild type and ApoE-deficient mice at the level of plasma biochemical markers. Levels of cholesterol and HDL increased in the plasma of elderly ApoE−/− mice and were markedly higher than in the plasma of elderly wild type animals. On the other hand, the activity of alanine transaminase (ALT) decreased in the plasma of elderly ApoE−/− mice and was markedly lower than in the plasma of elderly wild type animals.

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Effect of Ethanol Solvents on Total Phenolic Content and Antioxidant Properties of Seed Extracts of Fenugreek (Trigonella foenum-graecum L.) Varieties and Determination of Phenolic Composition by HPLC-ESI-MS

2021

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Fenugreek (Trigonella foenum-graecum L.) is one of the oldest cultivated plants grown for its leaves and seeds that are used for both culinary and medicinal purpose. This study aims to evaluate the effect of ethanol concentration (30, 50, 70 and 96% (v/v) of ethanol in water) as a solvent for the extraction of total phenolic content (TPC) and antioxidant properties (antiradical activity (ARA), transition metal reducing power (TMRP), iron chelating ability (ICA)) of seed extracts of spring variety Ovari 4 (FSV) and winter variety PSZ.G.SZ (FWV) fenugreek, and separate and identify the major phenolics of the extracts by HPLC-ESI-MS. The results indicated that 70% ethanol solution resulted in the maximum amount of TPC for both FSV and FWV seeds. The TPC decreased in the treatments in the following order: 70% ethanol > 96% ethanol > 50% ethanol > 30% ethanol, whereas extraction yield changed in a different manner: 30% ethanol > 50% ethanol > 70% ethanol > 96% ethanol. The extracts from seeds of both fenugreek varieties obtained with 70% and 96% ethanol showed equal high RSA while superior TMRP and ICA were observed in 70% ethanol extracts. The TMRP and ICA were strongly correlated with TPC for both varieties. The correlation between RSA and TPC was high, but not significant. Thus, the obtained data indicate the 70% ethanol solvent suitability for efficient extraction of phenolic compounds from seeds of the FWV and FSV. According to an HPLC-ESI-MS analysis, the polyphenolic profiles of fenugreek are presumably formed by flavone C-glycosides with apigenin or luteolin as aglycone linked with different glycones. High antioxidant activity of FWV seeds can be an adaptation to cold stress of the winter variety aimed at strengthening the antioxidant defense of the germinating seeds.

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Fenugreek (Trigonellafoenum-graecum L.) Seeds Dietary Supplementation Regulates Liver Antioxidant Defense Systems in Aging Mice

Cited by 6 publications

References 20 publications

Exploring the Medicinally Important Scondary Metabolites Landscape Through the Lens of Transcriptome Data in Fenugreek (Trigonella Foenum Graecum L.)

Exploring the Medicinally Important Scondary Metabolites Landscape Through the Lens of Transcriptome Data in Fenugreek (Trigonella Foenum Graecum L.)

Aging-Related Changes in the Ultrastructure of Hepatocytes and Cardiomyocytes of Elderly Mice Are Enhanced in ApoE-Deficient Animals

Effect of Ethanol Solvents on Total Phenolic Content and Antioxidant Properties of Seed Extracts of Fenugreek (Trigonella foenum-graecum L.) Varieties and Determination of Phenolic Composition by HPLC-ESI-MS

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