Fermentable sugars production from lignocellulosic materials hydrolysis by thermophilic enzymes from Bacillus subtilis J12

Kuancha, Chutima; Sukklang, Sompong; Detvisitsakun, Chanitchote; Chanton, Sudathip; Apiraksakorn, Jirawan

doi:10.1016/j.egypro.2017.10.084

Cited by 6 publications

(2 citation statements)

References 15 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…One study concluded that signal peptide and propeptide optimization could enhance secretion to increase heterologous protein production in L. lactis [21]. Additionally, the CMC activity of HT1/pMG36e- usp 45- egl 3 was higher than that of Clostridium thermocellum (1118 vs 140 mU/mL) and Bacillus subtilis J12 (1118 vs 165 mU/mL) [40, 41]. HT2 had higher filter paper activity than the average activity of cellulolytic rumen Enterococcus strains (236 vs 212 mU/mL) and T. reesei (236 vs 190 mU/mL) [4, 42].…”

Section: Discussionmentioning

confidence: 99%

Enhancement of lignocellulosic degradation in high-moisture alfalfa via anaerobic bioprocess of engineered Lactococcus lactis with the function of secreting cellulase

Liu

Zhao

et al. 2019

Biotechnol Biofuels

View full text Add to dashboard Cite

Background Butyric fermentation and a substantial loss of dry matter (DM) often occur in alfalfa silage during the rainy season, which is not conducive to subsequent biofuel production. Currently, there have been negative effects on the combination of cellulases and lactic acid bacteria (LAB) on processing high-moisture alfalfa silage; however, transgenically engineered LAB strains that secrete cellulase have been proposed as an alternative approach to avoid the above problem. The objective of the present study was to construct engineered Lactococcus lactis strains with high-efficiency secretory-expressing cellulase genes from Trichoderma reesei and to investigate the effects of the combination of transgenically engineered L. lactis strains HT1 / pMG36e- usp 45- bgl 1, HT1 / pMG36e- usp 45- cbh 2, and HT1 / pMG36e- usp 45- egl 3 (HT2) on fermentation quality, structural carbohydrate degradability and nonstructural carbohydrate fermentation kinetics of high-moisture alfalfa silage treated without additive as a negative control (Control), or/and with cellulase (EN), wild-type L. lactis subsp. lactis MG1363 (HT1) and the combination of HT1 and EN (HT1 + EN) as positive additive controls. Results Engineered L. lactis strains were successfully constructed and efficiently secreted endoglucanase (1118 mU/mL), cellobiohydrolase (222 mU/mL), and β-glucosidase (131 mU/mL) and had high filter paper activity (236 mU/mL). Ensiling experiments verified that HT2 obtained the highest fermentation quality score (83.6) and most efficiently processed high-moisture alfalfa silage, demonstrated by a low pH (4.49) and ammonia-N content (106 g/kg nitrogen) and a high lactic acid content (67.1 g/kg DM) and without butyric acid. Change curves of structural carbohydrates revealed that HT2 degraded more lignocelluloses, demonstrated by the lowest contents of neutral detergent fibre, acid detergent fibre, cellulose and hemicellulose after ensiling for 60 days. Kinetic analysis showed that the most residual water-soluble carbohydrates, glucose, fructose and xylose generated by lignocellulose degradation were produced by HT2, followed by HT1 + EN. The HT2-treated silages had the highest DM recovery, had the fewest Clostridia spores, emitted a fragrance and were not sticky. Conclusion HT2 improved the conversion of lignocellulose to sugars and processed high-moisture alfalfa silage efficiently. This is a novel strategy that can be used to enhance lignocellulosic degradation in high-moisture alfalfa via a bioprocess with transgenically engineered L. lactis ...

show abstract

Section: Discussionmentioning

confidence: 99%

Enhancement of lignocellulosic degradation in high-moisture alfalfa via anaerobic bioprocess of engineered Lactococcus lactis with the function of secreting cellulase

Liu

Zhao

et al. 2019

Biotechnol Biofuels

View full text Add to dashboard Cite

show abstract

“…Artigo de revisão sobre a importância da aplicação de técnicas moleculares para a produção de bioetanol (Kuancha et al, 2017) Fermentable sugars production from lignocellulosic materials hydrolysis by thermophilic enzymes from Bacillus subtilis J12 Artigo de pesquisa que apresenta alta eficiência na aplicação de Bacillus subtilis para a produção de açúcares (Agrawal et al, 2017) Investigating the enzyme-lignin binding with surfactants for improved saccharification of pilot scale pretreated wheat straw Artigo de pesquisa que investigou uma enzima específica para a prétratar palha de trigo (A. M. H. Prospecção tecnológica do capim elefante e sua relevância como matéria-prima para a produção energética Artigo de revisão que apresenta a importância do CE para produção de energias renováveis (Niemi et al, 2017)…”

Section: Application Of Pretreatment Fermentation and Molecular Techniques For Enhancing Bioethanol Production From Grass Biomass -A Reviunclassified

Lógica fuzzy aplicada ao pré-tratamento do capim-elefante: uma revisão bibliográfica sistemática

Godinho

Caneppele

2021

RSD

View full text Add to dashboard Cite

A economia global é diretamente dependente dos combustíveis fósseis, consumindo um total de aproximadamente 70% destas fontes de energia. Contudo, as energias renováveis, principalmente os biocombustíveis produzidos pela biomassa estão ganhando espaço, com destaque para o capim-elefante, que vem se destacando na produção de bioetanol no Brasil. No entanto, antes de ocorrer a fermentação para a produção do bioetanol, a biomassa deverá passar por um processo chamado de pré-tratamento. Por isso, quando se analisa os processos químicos, a otimização nas variáveis para o pré-tratamento é fundamental, assim surge a necessidade de aplicar um sistema de modelagem matemática como a lógica nebulosa ou fuzzy. Diante desse contexto, o objetivo desse artigo é identificar como está configurado o estado do conhecimento sobre a aplicação da lógica fuzzy no pré-tratamento alcalino no capim-elefante. Com a realização da pesquisa utilizando a Revisão Bibliográfica Sistêmica (RBS), foi possível identificar diversas oportunidades da aplicação da lógica fuzzy nos pré-tratamentos alcalinos no capim-elefante. Dos 86 documentos analisados nesta pesquisa, 100% foram de artigos de pesquisa e de revisão bibliográfica, o que demonstra uma relevância na academia ao uso do capim-elefante na produção bioetanol e, da mesma forma, que as pesquisas sobre a aplicação de pré-tratamento nas biomassas vem ganhando importância nos últimos anos, sendo cada vez mais estudada e desenvolvida por pesquisadores da área. Apesar de não apresentar nenhum artigo com referência específica da utilização da lógica fuzzy no pré-tratamento alcalino do capim-elefante, isso abre um grande leque para novas pesquisas utilizando a lógica nebulosa.

show abstract

Lignocellulolytic Enzymes from Thermophiles

Sharma

Vasanth

2018

Sustainable Biotechnology- Enzymatic Resources of Renewable Energy

View full text Add to dashboard Cite

Fermentable sugars production from lignocellulosic materials hydrolysis by thermophilic enzymes from Bacillus subtilis J12

Cited by 6 publications

References 15 publications

Enhancement of lignocellulosic degradation in high-moisture alfalfa via anaerobic bioprocess of engineered Lactococcus lactis with the function of secreting cellulase

Enhancement of lignocellulosic degradation in high-moisture alfalfa via anaerobic bioprocess of engineered Lactococcus lactis with the function of secreting cellulase

Lógica fuzzy aplicada ao pré-tratamento do capim-elefante: uma revisão bibliográfica sistemática

Lignocellulolytic Enzymes from Thermophiles

Contact Info

Product

Resources

About