2020
DOI: 10.4014/jmb.2008.08005
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Fermentation and Metabolic Pathway Optimization to De Novo Synthesize (2S)-Naringenin in Escherichia coli

Abstract: Flavonoids have diverse biological functions in human health. All flavonoids contain a common 2phenyl chromone structure (C6-C3-C6) as a scaffold. Hence, in using such a scaffold, plenty of highvalue-added flavonoids can be synthesized by chemical or biological catalyzation approaches. (2S)-Naringenin is one of the most commonly used flavonoid scaffolds. However, biosynthesizing (2S)naringenin has been restricted not only by low production but also by the expensive precursors and inducers that are used. Herein… Show more

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Cited by 39 publications
(38 citation statements)
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“…The pharmacological activities associated with naringenin have been attributed to its ability to suppress oxidative stress by scavenging free radicals generated during various basal metabolic conditions [ 9 ]. Presently, attempts are made for the de novo synthesis of pharmacologically numerous active flavonoids, including naringenin from Escherichia coli , in order to ensure their cost-effective high production [ 10 ]. In this article, we attempt to review the available scientific literature on various biological and pharmacological properties of naringenin.…”
Section: Introductionmentioning
confidence: 99%
“…The pharmacological activities associated with naringenin have been attributed to its ability to suppress oxidative stress by scavenging free radicals generated during various basal metabolic conditions [ 9 ]. Presently, attempts are made for the de novo synthesis of pharmacologically numerous active flavonoids, including naringenin from Escherichia coli , in order to ensure their cost-effective high production [ 10 ]. In this article, we attempt to review the available scientific literature on various biological and pharmacological properties of naringenin.…”
Section: Introductionmentioning
confidence: 99%
“…In recent years, (2 S )-naringenin has attracted increasing attention because of the antioxidation and anti-inflammatory properties of its derivatives . The biosynthesis of (2 S )-naringenin in microorganisms such as Saccharomyces cerevisiae with metabolic engineering and synthetic biology has become popular, and considerable research progress has been made. The advantages of microbial synthesis include the short generation time, ease of culture, amenability to large-scale fermentation, high production intensity, etc . Moreover, as well as its high tolerance of harsh industrial conditions and resistance to phage infection, S. cerevisiae can express plant-derived enzymes that perform the complex reactions involved in the synthesis of different flavonoids.…”
mentioning
confidence: 99%
“…7−9 The advantages of microbial synthesis include the short generation time, ease of culture, amenability to large-scale fermentation, high production intensity, etc. 10 Moreover, as well as its high tolerance of harsh industrial conditions and resistance to phage infection, S. cerevisiae can express plant-derived enzymes that perform the complex reactions involved in the synthesis of different flavonoids. (2S)-Naringenin has been glycosylated, prenylated, and hydroxylated in S. cerevisiae to produce vitexin, 11 8prenylnaringenin, 12 and dihydromyricetin, 13 respectively, demonstrating that S. cerevisiae is a promising host for the production of different flavonoids.…”
mentioning
confidence: 99%
“…In order to efficiently produce flavonoids, it is important to balance the biosynthesis of flavonoids and cell growth. Recently, an induction-free system to de novo biosynthesize naringenin from glucose by E. coli was established, and this system could automatically balance the production of naringenin, the accumulation of p-coumaric acid, and cell growth [61]. Because the direct precursor of malonyl-CoA is acetyl-CoA, the biosynthesis of acetyl-CoA is closely related to carbon source and acetic acid, so carbon source and acetic acid can be used to regulate the synthesis of acetyl-CoA and then the synthesis of malonyl-CoA.…”
Section: Microbial Mono-culture For Flavonoid Synthesismentioning
confidence: 99%
“…Because the direct precursor of malonyl-CoA is acetyl-CoA, the biosynthesis of acetyl-CoA is closely related to carbon source and acetic acid, so carbon source and acetic acid can be used to regulate the synthesis of acetyl-CoA and then the synthesis of malonyl-CoA. In order to increase the production of malonyl-CoA at the fermentation level, the concentration of glucose, glycerol, and potassium acetate (KAc) in the medium was optimized [61]. After optimization, the yield of naringenin could reach 588 mg/L, which was the highest titer reported in E. coli so far.…”
Section: Microbial Mono-culture For Flavonoid Synthesismentioning
confidence: 99%