Fermentation-induced variation in heat and oxidative stress phenotypes of Lactococcus lactis MG1363 reveals transcriptome signatures for robustness

Dijkstra, Annereinou; Alkema, Wynand; Starrenburg, Marjo; Hugenholtz, Jeroen; Hijum, Sacha A. F. T. van; Bron, Peter A.

doi:10.1186/s12934-014-0148-6

Cited by 27 publications

(46 citation statements)

References 38 publications

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“…lactis MG1363, correlated with increased oxidative stress tolerance. Subsequent experiments using cysteine-free medium, known to enhance metC-cysK expression (635,636), confirmed a role for these genes and the corresponding metabolic pathway in oxidative stress robustness (593). Similarly, TPM of the Lb.…”

Section: Gene Function Discovery By Phenotype Diversity Mining Stratementioning

confidence: 80%

“…For example, aerobic fermentation of Lc. lactis MG1363 stimulated enhanced heat stress tolerance, whereas fermentation at elevated temperatures enhanced the survival of the cultures under oxidative stress conditions (593). Increased NaCl concentrations during fermentation induced a reduction of intestinal stress tolerance in Lb.…”

Section: Gene Function Discovery By Phenotype Diversity Mining Stratementioning

confidence: 99%

“…Such multiple "stressomics" approaches are highly valuable for comprehensive stress response analyses, as they not only identify the genes directly involved in robustness and/or stress survival but also can reveal the regulatory networks involved. Moreover, the availability of several transcriptome profiles for a strain grown under different (stress-related) conditions (592,593) allows identification of genes that are coregulated. This information can then be used to reconstruct gene regulatory (stress) networks and regulons (351).…”

Section: Functional Genomic Approaches To Unravel the Lab Stressomementioning

confidence: 99%

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Stress Physiology of Lactic Acid Bacteria

Papadimitriou

Alegría

Bron

et al. 2016

Microbiol Mol Biol Rev

541

404

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SUMMARYLactic acid bacteria (LAB) are important starter, commensal, or pathogenic microorganisms. The stress physiology of LAB has been studied in depth for over 2 decades, fueled mostly by the technological implications of LAB robustness in the food industry. Survival of probiotic LAB in the host and the potential relatedness of LAB virulence to their stress resilience have intensified interest in the field. Thus, a wealth of information concerning stress responses exists today for strains as diverse as starter (e.g.,Lactococcus lactis), probiotic (e.g., severalLactobacillusspp.), and pathogenic (e.g.,EnterococcusandStreptococcusspp.) LAB. Here we present the state of the art for LAB stress behavior. We describe the multitude of stresses that LAB are confronted with, and we present the experimental context used to study the stress responses of LAB, focusing on adaptation, habituation, and cross-protection as well as on self-induced multistress resistance in stationary phase, biofilms, and dormancy. We also consider stress responses at the population and single-cell levels. Subsequently, we concentrate on the stress defense mechanisms that have been reported to date, grouping them according to their direct participation in preserving cell energy, defending macromolecules, and protecting the cell envelope. Stress-induced responses of probiotic LAB and commensal/pathogenic LAB are highlighted separately due to the complexity of the peculiar multistress conditions to which these bacteria are subjected in their hosts. Induction of prophages under environmental stresses is then discussed. Finally, we present systems-based strategies to characterize the “stressome” of LAB and to engineer new food-related and probiotic LAB with improved stress tolerance.

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Section: Gene Function Discovery By Phenotype Diversity Mining Stratementioning

confidence: 80%

Section: Gene Function Discovery By Phenotype Diversity Mining Stratementioning

confidence: 99%

Section: Functional Genomic Approaches To Unravel the Lab Stressomementioning

confidence: 99%

See 1 more Smart Citation

Stress Physiology of Lactic Acid Bacteria

Papadimitriou

Alegría

Bron

et al. 2016

Microbiol Mol Biol Rev

541

404

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“…They can be used as probiotics that confer health benefits to host through oral administration (Masood, Qadir, Shirazi, & Khan, 2011), as fermentation starter culture for cheese and yogurt production (Dijkstra et al, 2014), and as bio-preservatives for adding in animal feeds to inhibit the growth of pathogens (Schnürer & Magnusson, 2005). The dry powder form compared to liquid culture facilitates the subsequent processing procedures such as pelletization or tabletting, as well as transportation Peighambardoust, Golshan Tafti, & Hesari, 2011).…”

Section: Introductionmentioning

confidence: 99%

The mechanisms of the protective effects of reconstituted skim milk during convective droplet drying of lactic acid bacteria

Zheng

Duan

et al. 2015

Food Research International

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“…lactis subsp. cremoris strain MG1363 [10], oxygen level and fermentation temperature strongly affect subsequent survival during heat and oxidative stress assays, respectively [11]. Furthermore, by applying a transcriptome-phenotype matching approach, we revealed transcriptome signatures associated with robustness towards heat and oxidative stress, which could function as indicators for robustness.…”

Section: Introductionmentioning

confidence: 99%

Strain-Dependent Transcriptome Signatures for Robustness in Lactococcus lactis

et al. 2016

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Recently, we demonstrated that fermentation conditions have a strong impact on subsequent survival of Lactococcus lactis strain MG1363 during heat and oxidative stress, two important parameters during spray drying. Moreover, employment of a transcriptome-phenotype matching approach revealed groups of genes associated with robustness towards heat and/or oxidative stress. To investigate if other strains have similar or distinct transcriptome signatures for robustness, we applied an identical transcriptome-robustness phenotype matching approach on the L. lactis strains IL1403, KF147 and SK11, which have previously been demonstrated to display highly diverse robustness phenotypes. These strains were subjected to an identical fermentation regime as was performed earlier for strain MG1363 and consisted of twelve conditions, varying in the level of salt and/or oxygen, as well as fermentation temperature and pH. In the exponential phase of growth, cells were harvested for transcriptome analysis and assessment of heat and oxidative stress survival phenotypes. The variation in fermentation conditions resulted in differences in heat and oxidative stress survival of up to five 10-log units. Effects of the fermentation conditions on stress survival of the L. lactis strains were typically strain-dependent, although the fermentation conditions had mainly similar effects on the growth characteristics of the different strains. By association of the transcriptomes and robustness phenotypes highly strain-specific transcriptome signatures for robustness towards heat and oxidative stress were identified, indicating that multiple mechanisms exist to increase robustness and, as a consequence, robustness of each strain requires individual optimization. However, a relatively small overlap in the transcriptome responses of the strains was also identified and this generic transcriptome signature included genes previously associated with stress (ctsR and lplL) and novel genes, including nanE and genes encoding transport proteins. The transcript levels of these genes can function as indicators of robustness and could aid in selection of fermentation parameters, potentially resulting in more optimal robustness during spray drying.

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Fermentation-induced variation in heat and oxidative stress phenotypes of Lactococcus lactis MG1363 reveals transcriptome signatures for robustness

Cited by 27 publications

References 38 publications

Stress Physiology of Lactic Acid Bacteria

Stress Physiology of Lactic Acid Bacteria

The mechanisms of the protective effects of reconstituted skim milk during convective droplet drying of lactic acid bacteria

Strain-Dependent Transcriptome Signatures for Robustness in Lactococcus lactis

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