Fermentation medium for collagenase production by Penicillium aurantiogriseum URM4622

Abstract

Medium composition and culture conditions for maximal collagenase production by Penicillium aurantiogriseum URM4622 were optimized using a response surface approach. A full two-level design on three factors (initial medium pH, soybean flour concentration, and temperature) was employed to identify the most significant fermentation parameters for collagenase production, and a subsequent central composite design (CCD) was used to find the optimal levels of the two most significant factors (initial medium pH and soybean flour concentration). The design results indicated that the initial medium pH and the temperature had significant negative main effects, whereas the substrate concentration had a positive effect on the collagenase production. The maximum collagenolytic activity predicted by the fitted response surface was expected to occur at pH 7.21, 1.645% soybean flour concentration and 24°C. Three replicate experiments were run at these conditions and yielded an activity response of 283.36 ± 1.33 U, which not only is the highest obtained in this study but also represents a 5-fold increase over the lowest response observed in the initial design. Since all experiments were carried out with an inexpensive substrate, the final results point out to a cost-effective medium for collagenase production with potential industrial-scale applications.

Production and Characterization of Collagenase by Penicillium sp. UCP 1286 Isolated From Caatinga Soil

“…It is known that the costs of culture media and substrates are key factors on the production of industrial enzymes being thus necessary to develop processes that make use of inexpensive and easy to prepare substrates [8]. The use, as in the present work, of a low cost and readily available substrate, as is the case of gelatin and trace elements, may be an important step in the advancement of these processes.…”

“…The production effectiveness was evaluated by volumetric collagenolytic activity due to the industrial relevance of this parameter [8]. In addition, the enzyme concentration remained constant (a slight increase was observed) for the first 84 hours, most likely caused by the fungus need to get adapted to the poor culture medium, as compared to the other media listed in Table 4.…”

“…native and denatured collagens [5,6]. These enzymes can degrade native triple helix of collagen to small fragments and play an important role in connective tissue metabolism [7,8]. Collagenolytic enzymes are a kind of proteases that are related to various physiological and pathological processes and have several applications in industry, medicine and biotechnology [6,[9][10][11].…”

“…Studies have reported the biosynthesis of collagenases by fungi belonging to the different genera, such as Aspergillus, Cladosporium, Alternaria, Penicillium [8,[20][21][22], Candida [23], Microsporum [24] and Rhizoctonia [14]. Species of Penicillium genus have a higher biotechnological potential compared to other genera cited, both for production of proteases and other enzymes, as they have the capacity of growth in different culture conditions, using a wide variety of substrates as nutrients [25].…”

Antimicrobial and radical scavenging properties of bovine collagen hydrolysates produced by Penicillium aurantiogriseum URM 4622 collagenase

“…The soybean flour medium described by Lima et al (2011b), composed of 1.6 % (w/v) filtered soybean flour (SF), 0.1 % (w/v) NH 4 Cl, 0.06 % (w/v) MgSO 4 ·7H 2 O, 0.435 % (w/v) K 2 HPO 4 , 0.01 % (w/v) glucose, and 1.0 % (v/v) mineral solution, was used for collagenase production.…”